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Citations for Qiagen :
1001 - 1050 of 1556 citations for High mobility group protein B1 HMGB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... high-quality genomic DNA was isolated from whole epithelia using the DNeasy Blood and Tissue Kit per manufacturer protocol (Qiagen). PCR products were generated using REDTaq® polymerase and LSL excision primers ...
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bioRxiv - Microbiology 2024Quote: De novo reconstruction of plasmid sequences was performed by first isolating high molecular weight DNA with the Qiagen Genomic-tip 20/G protocol (Qiagen). Libraries were constructed using the Rapid Barcoding kit (Oxford Nanopore Technologies (ONT) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All PCRs were performed using master mixes of high-fidelity polymerases (Phusion or Q5) and purified with the QIAquick PCR purification kit (Qiagen). pUISacB was extracted from overnight cultures using the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Biophysics 2020Quote: ... All proteins were purified by affinity chromatography on Ni-NTA agarose (Qiagen), followed by incubation with TEV protease at 4 °C coupled with dialysis into buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of these tagged proteins was performed using Ni-NTA beads (Qiagen) or Glutathione Sepharose™ 4B beads (GE healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... Functional analysis of differentially abundant proteins was performed with IPA (QIAGEN Inc.)[14] ...
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bioRxiv - Microbiology 2021Quote: ... 100 µL of washed magnetic beads (BioMag Protein A, Qiagen, Hilden, Germany) resuspended in 100 µL TN-buffer were added to each sample (total volume of 750 µL ...
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bioRxiv - Immunology 2020Quote: ... Protein purification was undertaken using nickel-affinity chromatography (Qiagen, Valencia, CA, USA) and endotoxin was removed by two-phase extraction with Triton X-114 22 ...
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bioRxiv - Molecular Biology 2020Quote: Total protein was extracted using the Qiagen All prep mini kit (Qiagen) after elution through the RNA spin column ...
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bioRxiv - Cell Biology 2019Quote: ... Both proteins were affinity purified using Ni-NTA agarose beads (Qiagen, 30250) followed by gel filtration with a Superdex 200 HiLoad 16/600 column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... Fluorescent protein was bound to Ni-NTA Superflow columns (Qiagen, Hilden, Germany) equilibrated with 50 mM NaH2PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... The eluted protein complexes were further purified by Ni2+–NTA (Qiagen, Germany) affinity and/or anti-Flag M2 affinity chromatography (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted protein was incubated with pre-equilibrated Strep-Tactin Superflow Plus (Qiagen) and gentle rotation at 4°C for 1 h in binding buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved protein was further purified by reloading into Ni-NTA resin (QIAGEN). The flow through was collected ...
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bioRxiv - Biophysics 2020Quote: ... and the fusion protein was purified using a Ni-NTA resin (Qiagen). MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen) ...
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bioRxiv - Biophysics 2022Quote: ... and proteins were purified by two successive nickel affinity chromatography (Qiagen®) steps ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified by Ni-affinity chromatography (Ni-NTA agarose, Qiagen) followed by StrepTactin affinity chromatography (StrepTactin Sepharose high performance ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant protein was purified by Ni-NTA affinity chromatography column (QIAGEN).
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bioRxiv - Biophysics 2022Quote: ... The solubilized protein was incubated with Ni-NTA agarose (QIAGEN, Hilden, Germany) for several hours ...
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bioRxiv - Microbiology 2022Quote: ... 6XHis-tag fusion proteins were purified using Ni-NTA affinity chromatography (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The 6xHis tagged fusion protein was purified with Ni-NTA Agarose (Qiagen), as described in [13] ...
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bioRxiv - Microbiology 2021Quote: Proteins were purified by gravity flow using Ni-NTA agarose (Qiagen, 30210) in 2 mL chromatography columns (Bio Rad ...
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bioRxiv - Genomics 2022Quote: ... 300 μl of Protein Kinase Digestion (PKD) buffer (Qiagen, Cat. No. 169021771) was added to sample tubes ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant protein in the supernatant was purified via Ni2+-NTA (Qiagen, UK) column [20].
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bioRxiv - Synthetic Biology 2021Quote: ... the supernatants containing denatured proteins were mixed with Ni-NTA resin (Qiagen) and incubated for 60 min at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... the enriched proteins were incubated with 0.5 U/μl RNase A (Qiagen) and 20 U/μl RNase T1 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Proteases were precipitated using 200 ul of Protein Precipitation Solution (Qiagen, 158910) and centrifuged for 10 min at 12 000 g at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Cell Biology 2021Quote: ... 500-800μg of protein were incubated with Ni-NTA agarose beads (Qiagen) at 4° C overnight ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were purified from lysates on Ni-NTA columns (Qiagen).
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bioRxiv - Immunology 2021Quote: ... DNA extracts were prepared using Allprep Bacterial DNA/RNA/Protein Kit (Qiagen) following the manufacturer’s instructions and further submitted to Genewiz for bacterial 16S-EZ sequencing (V3 and V4 hypervariable regions ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was first purified by FPLC using a Ni-NTA column (Qiagen) followed by a heparin column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified from the supernatant via nickel affinity chromatography (Qiagen). The protein was further purified by a Hiload 16/60 Superdex 200 column (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: SacSm-containing protein sample was loaded on a Ni-NTA Agarose (Qiagen) column equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... for GST-tagged proteins or nickel– nitriloacetic acid (Ni-NTA) agarose (Qiagen) for His-tagged proteins (2 hr ...
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bioRxiv - Biochemistry 2024Quote: ... After the separation of nanodiscs and soluble proteins on Ni-NTA (Qiagen), the same proportions of unbound material and imidazole-eluted material were analyzed (Fig.S5) ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). Eluted protein was concentrated and passed over a Superdex 200 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Immunology 2022Quote: ... The cleaved proteins were passed over a 1mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted with AllPrep DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins used for inhibition assays were purified using Ni-NTA resin (Qiagen) followed by size exclusion column chromatography (ÄKTA pure ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were purified using gravity flow Ni-NTA (Qiagen, Monheim, Germany) affinity chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... protein purification was carried out via IMAC using Ni-NTA agarose (Qiagen) equilibrated with TBSEq (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... coli was modified from the protein purification protocol of The QIAexpressionist (QIAGEN) as follows ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant proteins were purified over nickel-nitrilotriacetic acid (Ni-NTA) Agarose (Qiagen). Therefore ...
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bioRxiv - Biochemistry 2023Quote: ... All proteins were purified by affinity chromatography on Ni-NTA agarose (Qiagen), followed by incubation with TEV protease at 4 °C overnight coupled with dialysis into buffer A.
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bioRxiv - Cancer Biology 2023Quote: ... before RNA isolation using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Next RNA and protein were digested and DNA was recovered by Qiagen PCR purification kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen) supplemented with 0.4X NuPAGE™ LDS Sample Buffer (Thermofisher ...