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Citations for Qiagen :
1051 - 1074 of 1074 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Cys protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9-Cys wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... Functional pathway and network analysis of differentially expressed proteins were performed using Ingenuity Pathway Analysis (IPA) (Qiagen, Redwood City, CA, USA). The pathway and network score were based on the hypergeometric distribution and calculated with the right-tailed Fisher’s exact test.
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... After incubation microstructures were washed with PBS and RNA was isolated with Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen # 80004). cDNA was synthesized using the SuperScript VILO cDNA synthesis kit (ThermoFisher #11755250) ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested 4 days (96hr) after treatment and RNA extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, USA). Concentrations of RNA samples were analysed using Qubit fluorometric assays (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from biopsies of normal colonic tissues with the Allprep DNA/RNA/Protein Mini kit (Qiagen, Germantown, MD) and reverse-transcribed into cDNA with the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was again incubated with equilibrated Ni-NTA resin for 1 hour at 4°C to bind the His-tagged TEV and any uncut fraction of the protein (100µL Ni-NTA slurry per gram of frozen cell pellet, Qiagen cat#4561). The flowthrough containing the cut protein was then buffer exchanged into ion exchange A buffer (IEX-A ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein were purified from the homogenized tissues using the Allprep Mini kit according to the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA concentration was measured by Qubit using the RNA HS assay (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled protein in the soluble fraction was purified using Immobilized Metal Affinity Chromatography (IMAC) using standard methods (QIagen Ni-NTA resin). The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells treated for 48 h with 1 mM aspirin and 4 μM regorafenib using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen Cat# 80004) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Immunology 2023Quote: Total DNA and mRNA were isolated from the intestine samples collected during fish necropsy with the AllPrep DNA/RNA/Protein Kit (Qiagen, Hilden, Germany), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: TaqMan RT-qPCR targeting the envelope protein gene (E gene) was performed using the Quantitect Probe RT-PCR kit (Qiagen, Courtaboeuf, France). The model of primers/probe used was as follows ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma membrane proteins were purified from cultured astrocytes using a commercially available kit (Qproteome Cell Compartment Kit; Qiagen, Inc, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The left hippocampus was homogenized on ice in the lysis buffer from the AllPrep® DNA/RNA/Protein Mini Kit (QIAGEN, Hilden, Germany). A sample from the lysate was used to quantify total DNA and RNA content using the appropriate Qubit® Fluorometer kit (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were lysed and proteins simultaneously denatured in the appropriate lysis buffer according to the commercial kit DNeasy Blood & Tissue Kit (Qiagen, cat. no. 69504). Proteinase K then was added and after incubation in 55 °C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... This construct was co-transfected with the pVSV-G retroviral coat protein expression vector into GP2-293 packaging cells using Effectene (Qiagen Sciences, Inc.; Germantown, MD). At 24 h and again at 48 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were mechanically dissociated in lysis buffer and total RNA was extracted using the AllPrep DNA/RNA/protein mini Kit (Qiagen, USA, Cat. No. / ID: 80004) according to the manufacturer’s protocol ...