Labshake search
Citations for Qiagen :
851 - 900 of 1074 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: Total RNA was isolated from 50 mg of frozen human myocardial LV tissue samples using the miRNeasy Mini Kit (Qiagen: Hilden, DE). To remove all DNA ...
-
bioRxiv - Neuroscience 2024Quote: The expression of 84 PPAR target genes was assessed using the Human PPAR Targets RT2 Profiler PCR Array (Qiagen; 384-well format) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: RNA was extracted as total RNA from 5mg of postmortem human brain tissue using the miRNeasy Tissue/Cells Advanced Micro Kit (Qiagen, Hilden, Germany). RIN values were determined as previously described in the miRNA extraction procedure with RIN>5.5 as the RNA quality cut-off resulting in N=40 samples for which library preparation was performed ...
-
bioRxiv - Cancer Biology 2024Quote: Genome-wide methylation analysis was performed on 500 ng of DNA isolated from #14 human PM cell lines of different histotype using QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany) by the Infinium Human Methylation EPIC 850k (EPIC ...
-
bioRxiv - Cancer Biology 2020Quote: ... PCR array for human cell cycle (Cat#PAHS-020Z) and oncogenes and tumor suppressor genes (Cat#PAHS-502Z) was purchased from Qiagen (Valencia, CA, USA), and was used according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... To profile the expression of genes related to p53-mediated signal transduction based on the RT² Profiler™ PCR Array Human p53 Signaling Pathway (#330231, PAHS-027ZC, Qiagen, Hilden, Germany), reverse transcription and qPCR were performed using the RT2 First Strand Kit (#330401 ...
-
bioRxiv - Neuroscience 2024Quote: ... DNA was extracted from fresh frozen human brain tissue from the anterior cingulate or OCx using the DNeasy DNA extraction kit (Qiagen, Hilden, Germany, #69506), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... The clarified supernatant containing the target protein was incubated with pre-equilibrated Ni-NTA resin (Qiagen) for 30 min at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
-
bioRxiv - Molecular Biology 2021Quote: Proteins were purified from Sf21 insect cells by affinity purification with Ni-NTA agarose (Qiagen, 30230) and ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Ni+2-NTA agarose affinity chromatography according to standard protocol (Qiagen).
-
bioRxiv - Genomics 2021Quote: ... Total RNA was isolated from tumor samples using the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to standard protocol on the QiaCube (Qiagen ...
-
bioRxiv - Systems Biology 2021Quote: ... PBMCs were thawed on ice and then lysed to protein fraction using Allprep Spin Columns (Qiagen) according to the manufacturer’s instructions with the QIAshredder lysis option ...
-
STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Denaturing purification of the protein with urea was carried out using the Ni-NTA Agarose (Qiagen). Immunisation of rabbits was carried out at Eurogentec ...
-
bioRxiv - Microbiology 2021Quote: ... The LuxT-6xHis protein was purified from the clarified supernatant by Ni-NTA Superflow resin (Qiagen). Following washes with lysis buffer containing 20 mM Imidazole ...
-
bioRxiv - Cancer Biology 2021Quote: ... washed again and proteins were visualized by SuperSignal™ West Dura Extended Duration Substrate (Qiagen #34076)
-
bioRxiv - Biochemistry 2022Quote: ... Protein extract was subjected to IMAC by incubation with 2 ml Ni-NTA resin (Qiagen, Germany) per 50 ml of extract ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein extract (1 mg) was incubated with His6-RabD2c (1 μg) and Ni-NTA Agarose (QIAGEN) for 1 h at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... The protein sequences were aligned by MUSCLE [76] and visualized by CLC Genomic Workbench 8.5.1 (Qiagen). Subsequently ...
-
bioRxiv - Immunology 2022Quote: ... Protein expression was performed using standard protocols and purification was performed using Ni-NTA Agarose (Qiagen). Recombinant RelMtb protein (87 kDa ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins identified from proteomics were mapped to existing biological networks by Ingenuity Pathway Analysis (QIAGEN, CA)
-
bioRxiv - Neuroscience 2022Quote: Extraction of total DNA and RNA was performed using the Allprep DNA/RNA/Protein kit (Qiagen) according to the manufacturers protocol ...
-
bioRxiv - Systems Biology 2022Quote: ... RNA was isolated according to the manufacturer’s instructions (AllPrep DNA/RNA/Protein Mini, Qiagen, Austin, Texas). RNA quality was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... The fusion protein in the lysate was affinity-purified by Ni-NTA agarose beads (Qiagen, 30210) capturing HIS tag or by glutathione sepharose 4B beads (Amersham ...
-
bioRxiv - Immunology 2024Quote: ANKRD55 interactome proteins were analyzed with the use of Ingenuity Pathway Analysis (IPA) software from QIAGEN (https://digitalinsights.qiagen.com/IPA) ...
-
bioRxiv - Neuroscience 2023Quote: ... The protein was then purified on Ni-NTA affinity chromatography with Ni-NTA agarose resin (Qiagen) following the protocol described in Qiagen ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant containing each target protein was loaded onto a Ni-NTA (nitrilotriacetic acid) column (Qiagen) which was pre-equilibrated with the corresponding Lysis Buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... 500 mM KCl and the fusion proteins purified from clarified lysate through consecutive Ni-NTA (Qiagen) and HiTrap Q HP (GE Healthcare ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteins modified with 6×-His-Ub was purified using Ni-NTA Sepharose (142350243, Qiagen, Alameda, CA) on a rotating platform for 2 hours at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... and the supernatant was used for bacterial protein purification using Ni-NTA affinity resin (Qiagen: 30210).
-
bioRxiv - Plant Biology 2022Quote: Protein sequence alignments and phylogenetic analyses were obtained with the CLC Main Workbench v6 software (Qiagen). Phylogenetic tree was used from matrix pairwise data and using UPGMA algorithm ...
-
bioRxiv - Cancer Biology 2023Quote: DNA was isolated from all the cell lines using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). The samples were treated with sodium bisulfite to convert unmethylated cytosine residues to uracil via deamination using the EpiTect Bisulfite kit (Qiagen) ...
-
bioRxiv - Biochemistry 2023Quote: DNA was extracted from the insoluble protein pellet with the QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer protocol for DNA extraction from blood ...
-
bioRxiv - Immunology 2024Quote: ... Proteins and complexes were purified from the cell supernatants using Nickel-NTA affinity resin (Qiagen: 30210), followed by Superose 6 (Cytiva ...
-
bioRxiv - Immunology 2024Quote: ... The eluted compounds were subjected to RNA and protein isolation using QIAzol (Qiagen, Misusage, ON, Canada) following the manufacturer’s instruction ...
-
bioRxiv - Immunology 2024Quote: ... The supernatant from the sonicated proteins was purified by using Ni-NTA agarose (Qiagen, Düsseldorf, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... WT and mutant proteins were initially purified using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAgen). Subsequently ...
-
bioRxiv - Microbiology 2024Quote: ... 1% v/v sodium dodecyl-sulfate pH 8.0) for protein extraction or in RLT buffer (Qiagen) for RNA extraction ...
-
bioRxiv - Biochemistry 2024Quote: ... His-NusA-FASP or His-NusA-Degron fusion proteins were purified using Ni-NTA resin (Qiagen) using standard approaches and His-TEV protease was added to cleave the His-NusA tag from the PER2 peptides on column at 4°C overnight ...
-
bioRxiv - Synthetic Biology 2024Quote: The MED21 protein sequence was aligned to homologs using CLC Sequence Viewer 7 (QIAGEN, Aarhus, Denmark), a tree was constructed using a neighbor-joining method ...
-
bioRxiv - Cancer Biology 2024Quote: ... total RNA was extracted from MGM tissue specimens using the AllPrep DNA/RNA/Protein Kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... the UAS vector encoding the protein and the ubiquitin-Gal4 construct were transfected with Effectene (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... the ViiA 7™ Real-Time PCR system was used to run the Human Stem Cell RT² Profiler™ PCR Array (Qiagen, Hilden, Germany). For the analysis of cardiac differentiation-associated genes ...
-
bioRxiv - Evolutionary Biology 2021Quote: Protein sequence alignments and tree production were made using the CLC main work bench (ver. 5.5.1, Qiagen) software package ...
-
bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
-
bioRxiv - Molecular Biology 2020Quote: Nme2Cas9 protein was expressed in Rosetta (DE3) cells and purified using a Ni2+-NTA agarose column (QIAGEN) as previously described24 ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.2 mM TCEP) the expressed protein was extracted from the supernatant using NiNTA resin (QIAGEN, Germantown, MD). The resulting eluate from the NiNTA resin using lysis buffer supplemented with 250 mM Imidazole pH 7.4 ...
-
bioRxiv - Plant Biology 2021Quote: Alignments of histone DNA and protein sequences was performed using CLC Main Workbench software version 8.1 (QIAGEN). Predictions of intrinsic disorder were undertaken by PONDR (58) ...