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Citations for Qiagen :
651 - 700 of 1074 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then purified with Ni-NTA agarose beads (QIAGEN; 30210) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... we differentiated human stem cells for 3 days in mTeSR + 0.5 µM A8301 and extracted RNA with RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were used for preamplification and qPCR arrays (Rat Antibacterial Response, PARN-148ZE, and Human Antibacterial Response, PAHS-148ZC; Qiagen) were used for the expression analysis of 84 genes involved in innate antibacterial responses in human and rat respectively ...
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bioRxiv - Cell Biology 2021Quote: ... and transfected with 1 μg of a pcDNA3.1 vector containing human αSyn cDNA (provided by T. Baron, Anses, Lyon, France) using Effecten transfection reagent (Qiagen). Positive clones were selected using geneticin and cultured in Dulbecco’s modified Eagle’s medium (DMEM/F-12 ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA expression of 84 apoptotic genes was analyzed using the RT2 Profilμer PCR Human Apoptosis Array (Qiagen, PAHS-012Z). Arrays were prepared according to the manufacturer’s protocols applied to the prepared cDNA samples ...
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bioRxiv - Bioengineering 2020Quote: ... each SeraCare panel member was diluted 1:10 in human plasma to a final volume of 100μL and processed by QIAGEN QIAamp viral mini kit ...
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: The human VCP cDNA was reverse transcribed from total RNA by using Omniscript RT kit (Qiagen Japan, Tokyo, Japan) extracted from A549 cells (RIKEN Cell Bank ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA targets were only included if biochemically confirmed using human tissue or non-species specific methods (sourced from QIAGEN’s curated Ingenuity Knowledge Base[48] ...
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bioRxiv - Immunology 2021Quote: ... followed by amplification and quantification using RT2 Profiler Human Innate and Adaptive Immune Response 96-well Array (Qiagen, 330231) with RT2 SYBR Green ROX qPCR Mastermix (Qiagen ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA was extracted from human and mouse feces-derived bacterial supernatant using the DNeasy powersoil kit (Qiagen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or postmortem human cerebellar vermis samples using either the RNeasy Kit or RNeasy Fibrous Tissue Kit following the manufacturer’s protocols (QIAGEN). For tissue RNA extraction ...
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bioRxiv - Microbiology 2023Quote: ... Human DNA from whole cell extracts of A549 cells was purified using the DNeasy Blood and Tissue kit (Qiagen). RNAseA (Thermo ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA was extracted from human tissue and cyst fluid using the QIAamp Mini kit (Qiagen, Hilden, Germany). Extractions were performed per manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: RNA was extracted from human tissue derived organoids and neutrophils using the RNeasy® Plus Mini Kit (Qiagen, #74104) with cDNA prepared using a SuperScript™ VILO™ cDNA synthesis kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The sequenced reads were mapped with the hg38 human genome and sequence analysis was done by the CLC genomics workbench (12.0.3, Qiagen Bioinformatics). Additionally ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from both NHP and human stool samples using the PowerFecal DNA Isolation Kit (Qiagen, Valencia, CA). Sequencing of the 16s small subunit ribosomal ribonucleic acid (SSU rRNA ...
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bioRxiv - Genetics 2023Quote: ... Raw FASTQ reads were aligned to the GRCh38 human genome using CLC Genomics Workbench version 22.0.2 software (Qiagen, USA). Differential gene expression was determined using the built-in tool in CLC Genomics workbench ...
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bioRxiv - Genetics 2024Quote: Total RNA from post-mortem adult human neural retina was extracted following manufacturer’s guidelines (RNeasy Mini kit®, Qiagen) followed by DNase treatment (ArcticZymes ...
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bioRxiv - Molecular Biology 2024Quote: ... as per the manufacturer’s instructions and profiled the most abundant 1092 miRNAs using human mirnome miscript miRNA PCR array (Qiagen). The data was analysed by ΔΔCT method of relative quantification using an analysis tool available at geneglobe.qiagen.com.
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bioRxiv - Microbiology 2024Quote: The RNAseq data were mapped to the human reference genome (GRCh38.109) using CLC Genomics Workbench 21.0 (QIAGEN, Aarhus, Denmark). The Voroni diagram was created using Python 3.11 and matplotkib 3.7.2 ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was extracted from FFPE MCF7 and human tumor tissue specimens using the QIAamp DNA FFPE Tissue kit (Qiagen). DNA was quantified using Qubit (Qiagen ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified using a Ni-NTA Superflow column (Qiagen, Netherlands, 30761) on a Äkta Express fast protein liquid chromatography system (FPLC ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transiently transfected with mammalian protein expressing plasmids using Effectene (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: DNA was extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and the soluble protein fractions were purified by Ni-NTA resin (Qiagen, # 30230). The purified fraction was concentrated ...
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bioRxiv - Biochemistry 2020Quote: ... The target proteins were purified with nickel chelate affinity resins (Ni-NTA, Qiagen). The protein samples were collected and purified by a Superdex 200 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen), amylose (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Whole DNA and proteins were extracted by the ALLprep Kit (Qiagen, Hilden, Germany), following manufacturer instructions.
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bioRxiv - Microbiology 2022Quote: ... and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen) following the instructions of the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was analyzed by immunoblotting using monoclonal anti-RGSH4 antibodies (Qiagen) to detect ABCG5 and polyclonal anti-hABCG8 antibodies (Novus Biologicals ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of protein were incubated with Ni-NTA agarose resin (Qiagen, 30210) for four hours ...
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bioRxiv - Immunology 2021Quote: ... The proteins from filtered culture supernatants were purified by Ni-NTA column (QIAGEN) and size exclusion chromatography (HiLoad Superdex 75 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified by metal affinity chromatography on a Ni2+-NTA column (Qiagen). Cell free extract was loaded onto column followed by two wash steps with wash buffer (Tris 50 mM ...
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bioRxiv - Biochemistry 2021Quote: ... Briefly proteins were purified by glutathione-Sepharose (Cytiva) or Ni-NTA agarose (Qiagen) affinity chromatography ...
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bioRxiv - Microbiology 2021Quote: ... and the protein was purified using a Ni–NTA column (Qiagen, Hilden, Germany) as reported previously 49 ...
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bioRxiv - Microbiology 2020Quote: ... The RBD protein was purified by affinity chromatography using Ni-NTA resin (QIAGEN), followed by size exclusion chromatography on a HiLoad Superdex 200 pg column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The protein interaction network analysis was performed using QIAGEN’S Ingenuity Pathways Analysis (QIAGEN’S Ingenuity pathway analysis ...
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under non-denaturing conditions using Ni-NTA resin (Qiagen) at 4 °C according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was isolated from the lysate using Ni-NTA Agarose resin (Qiagen) and was purified to homogeneity and >95% purity by size exclusion chromatography using a HiLoad Superdex 75 pg 16/600 column (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were purified through metal-affinity chromatography using Ni2+–NTA agarose beads (Qiagen). Purified protein aliquots were stored at -30°C in 50 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2023Quote: ... all proteins were purified using Ni-NTA (nickel-nitrilotriacetic acid) resin from QIAGEN. After the incubation of Expi293 media supernatant with the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were first purified using nickel-nitrilotriacetic acid (NTA) agarose resin (Qiagen), and the His8-SUMO tag was then removed by TEV protease (10:1 w/w ...
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bioRxiv - Physiology 2023Quote: ... Tissues were then lysed in protein isolation buffer using a tissue-lyser (Qiagen). Protein concentration was measured by Bradford assay ...
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bioRxiv - Microbiology 2023Quote: ... Digested protein was mixed with 3 ml of NTA super-flow resin (Qiagen) that had been pre-equilibrated in wash buffer ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity purified using Ni-NTA agarose (Qiagen; Table S4), eluted with 50 mM Tris-base ...
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bioRxiv - Microbiology 2023Quote: ... All nucleotide and protein sequences were analyzed using CLC Main Workbench 8.1.2 (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: SAINT Express protein hits were analyzed using Ingenuity Pathway Analysis (IPA) software (Qiagen). Significant hits were analyzed and enrichment z-score was calculated for different pathways ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified by immobilized metal affinity chromatography using Ni–NTA agarose (Qiagen) pre-equilibrated with lysis buffer in individual Econo-Pac gravity-flow columns (Bio-Rad) ...