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Citations for Qiagen :
601 - 650 of 1074 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Primary human Fibroblasts were washed with PBS before total RNA extraction using the RNeasy Plus Mini Kit (Qiagen).
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bioRxiv - Molecular Biology 2024Quote: RNA from human cell cultures was extracted from 6 well plates using RNeasy Plus micro kit (Qiagen, 74034). The plates were washed once with PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Reads from *.fastq files were mapped to the human reference genome (GRCh37) using CLC Genomics Workbench 12.0 (Qiagen).
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bioRxiv - Biophysics 2020Quote: ... All proteins were purified by affinity chromatography on Ni-NTA agarose (Qiagen), followed by incubation with TEV protease at 4 °C coupled with dialysis into buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... Functional analysis of differentially abundant proteins was performed with IPA (QIAGEN Inc.)[14] ...
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bioRxiv - Microbiology 2021Quote: ... 100 µL of washed magnetic beads (BioMag Protein A, Qiagen, Hilden, Germany) resuspended in 100 µL TN-buffer were added to each sample (total volume of 750 µL ...
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bioRxiv - Immunology 2020Quote: ... Protein purification was undertaken using nickel-affinity chromatography (Qiagen, Valencia, CA, USA) and endotoxin was removed by two-phase extraction with Triton X-114 22 ...
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bioRxiv - Molecular Biology 2020Quote: Total protein was extracted using the Qiagen All prep mini kit (Qiagen) after elution through the RNA spin column ...
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bioRxiv - Biophysics 2022Quote: ... Protein was purified through affinity chromatography (Ni-NTA His-Bind resin, Qiagen), followed by gel filtration chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Biochemistry 2021Quote: ... Eluted protein was incubated with pre-equilibrated Strep-Tactin Superflow Plus (Qiagen) and gentle rotation at 4°C for 1 h in binding buffer containing 50 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved protein was further purified by reloading into Ni-NTA resin (QIAGEN). The flow through was collected ...
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bioRxiv - Biophysics 2020Quote: ... and the fusion protein was purified using a Ni-NTA resin (Qiagen). MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen) ...
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bioRxiv - Biophysics 2022Quote: ... and proteins were purified by two successive nickel affinity chromatography (Qiagen®) steps ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified by Ni-affinity chromatography (Ni-NTA agarose, Qiagen) followed by StrepTactin affinity chromatography (StrepTactin Sepharose high performance ...
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bioRxiv - Biophysics 2022Quote: ... The solubilized protein was incubated with Ni-NTA agarose (QIAGEN, Hilden, Germany) for several hours ...
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bioRxiv - Microbiology 2022Quote: ... 6XHis-tag fusion proteins were purified using Ni-NTA affinity chromatography (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Proteins were purified by gravity flow using Ni-NTA agarose (Qiagen, 30210) in 2 mL chromatography columns (Bio Rad ...
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bioRxiv - Genomics 2022Quote: ... 300 μl of Protein Kinase Digestion (PKD) buffer (Qiagen, Cat. No. 169021771) was added to sample tubes ...
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bioRxiv - Synthetic Biology 2021Quote: ... the supernatants containing denatured proteins were mixed with Ni-NTA resin (Qiagen) and incubated for 60 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Proteases were precipitated using 200 ul of Protein Precipitation Solution (Qiagen, 158910) and centrifuged for 10 min at 12 000 g at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was purified from cell supernatants using Ni-NTA resin (Qiagen, Germany), the eluted fractions containing S protein were pooled ...
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bioRxiv - Cell Biology 2021Quote: ... 500-800μg of protein were incubated with Ni-NTA agarose beads (Qiagen) at 4° C overnight ...
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bioRxiv - Immunology 2021Quote: ... DNA extracts were prepared using Allprep Bacterial DNA/RNA/Protein Kit (Qiagen) following the manufacturer’s instructions and further submitted to Genewiz for bacterial 16S-EZ sequencing (V3 and V4 hypervariable regions ...
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bioRxiv - Cell Biology 2020Quote: ... HIS-TRIM39 proteins were purified on Ni-NTA agarose beads (Qiagen, #1018244) and then eluted in a buffer containing 0.5 M imidazole before dialysis in PBS.
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bioRxiv - Biochemistry 2021Quote: ... Protein was first purified by FPLC using a Ni-NTA column (Qiagen) followed by a heparin column (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Immunology 2022Quote: ... The cleaved proteins were passed over a 1mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Biochemistry 2023Quote: ... All proteins were purified by affinity chromatography on Ni-NTA agarose (Qiagen), followed by incubation with TEV protease at 4 °C overnight coupled with dialysis into buffer A.
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bioRxiv - Cancer Biology 2023Quote: ... Proteins used for inhibition assays were purified using Ni-NTA resin (Qiagen) followed by size exclusion column chromatography (ÄKTA pure ...
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bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted with AllPrep DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA Superflow, Qiagen). Eluted protein was concentrated and passed over a Superdex 200 Increase 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... Next RNA and protein were digested and DNA was recovered by Qiagen PCR purification kit ...
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bioRxiv - Cell Biology 2023Quote: ... His10 proteins were purified by using Ni-NTA agarose (QIAGEN, Catalog # 30230), followed by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then purified with Ni-NTA agarose beads (QIAGEN; 30210) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA agarose, Qiagen) and then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen) supplemented with 0.4X NuPAGE™ LDS Sample Buffer (Thermofisher ...
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bioRxiv - Cancer Biology 2023Quote: ... before RNA isolation using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: SacSm-containing protein sample was loaded on a Ni-NTA Agarose (Qiagen) column equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified from the supernatant via nickel affinity chromatography (Qiagen). The protein was further purified by a Hiload 16/60 Superdex 200 column (Cytiva ...
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bioRxiv - Plant Biology 2022Quote: ... bound His-mSTIC2 protein was detected by anti His HRP conjugate (Qiagen) and ECL reaction (Pierce).
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bioRxiv - Plant Biology 2022Quote: ... His-tag proteins were purified using a Ni-NTA purification system (Qiagen) by following the specification of the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were purified using gravity flow Ni-NTA (Qiagen, Monheim, Germany) affinity chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... protein purification was carried out via IMAC using Ni-NTA agarose (Qiagen) equilibrated with TBSEq (50 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... coli was modified from the protein purification protocol of The QIAexpressionist (QIAGEN) as follows ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by affinity chromatography using Ni-NTA agarose (Qiagen) resin followed by his tag cleavage by TEV protease and gel filtration using Superdex 200 Increase 10/300 GL column (G.E ...
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bioRxiv - Biochemistry 2024Quote: ... Protein-digested DNA was purified using a PCR Clean-up kit (Qiagen). Purified DNA was treated with 100 nM TDP2 in 30 µL of 1X rCutsmart buffer (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... The fusion protein was purified using Ni-NTA Resin Spin Columns (QIAGEN). Eurogentec (Belgium ...
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bioRxiv - Biochemistry 2024Quote: ... His-NusA-tail fusion proteins were purified using Ni-NTA resin (Qiagen) using standard approaches and His-TEV protease was added to cleave the His-NusA tag from the tail on column at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were purified by glutathione-Sepharose (Cytiva) or Ni-NTA agarose (Qiagen) affinity chromatography ...