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Citations for Qiagen :
951 - 1000 of 1074 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and and concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... NusA–Strep tag–gene 60–6 × HIS proteins were purified by sequential chromatography on Ni-NTA agarose (Qiagen) and S-protein agarose (Novagen) ...
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bioRxiv - Neuroscience 2024Quote: Total DNA was extracted from snap-frozen hippocampi by using AllPrep DNA/RNA/Protein mini Kit (Qiagen, 80004), according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the lysates were cooled on ice and mixed with 200 µL of Protein Precipitation Solution (Qiagen ref. 158910) for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Cln1-His6 and its associated proteins were co-purified following the Ni-NTA Spin Kit procedure (Qiagen, Valencia, CA). Cell lysate from the CLN1-His6 strain was used as a negative control ...
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Neuroscience 2020Quote: The genes encoding protein groups significantly altered in epilepsy and control were analyzed using Ingenuity Pathway Analysis (IPA, Qiagen). We analyzed all differentially expressed proteins in the hippocampus (n=777) ...
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bioRxiv - Microbiology 2021Quote: ... and H2A.Z (fused with a maltose-binding protein -MBP) purification were performed by affinity chromatography using Ni-NTA agarose (Qiagen) and amylose resin (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was clarified by centrifugation at 13,000 rpm and protein was purified using Ni- NTA superflow resin (Qiagen), according to the manufacturer’s recommendations for a centrifugation-based protocol ...
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bioRxiv - Microbiology 2022Quote: ... Headless HA stalk proteins were expressed in 293F cells and purified using nickel-nitrilotriacetic acid agarose (no. 1018244, Qiagen) in 5-ml polypropylene columns (no ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Microbiology 2022Quote: ... Tissue lysate was further used for DNA extraction with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany). Total DNA extraction of seawater filters was done using the entire filter ...
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bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Synthetic Biology 2021Quote: ... After spinning down the insolubilized protein fraction the supernatant was loaded onto an equilibrated mini NiNTA spin column (Qiagen) and the flow-through was reloaded twice to maximize protein binding ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Genomics 2022Quote: ... DNA and RNA was extracted from fresh-frozen tissue samples using the AllPrep DNA/RNA/Protein minikit (Qiagen, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant SlERF.D7-6XHIS protein was purified by Ni2+gravity flow chromatography according to the manufactureer’s protocol (Ni-NTA Agarose, Qiagen) as described ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant RIN-6XHIS protein was purified by Ni2+ gravity flow chromatography according to the manufacturer’s protocol (Ni-NTA Agarose, Qiagen). For the EMSA assay ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from FACS-purified astrocytes from a single mouse brain using the QIAshredder and the All-prep DNA/RNA/protein Mini Kit according to the manufacturer’s protocol (Qiagen). Total RNA concentration was measured using a NanoVue PlusTM spectrophotometer (Biochrom) ...
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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bioRxiv - Genetics 2023Quote: ... Precipitated proteins were resuspended in 6M guanidine hydrochloride and incubated with 25 µL of Ni-NTA agarose beads (Qiagen) overnight ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed by NTA and then processed with AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen).
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from these samples according to the manufacturer’s instruction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). RNA was quantified using a Nanodrop-1000 spectrophotometer (Thermo-Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from hippocampus and pre-frontal cortex was purified with AllPrep DNA/RNA/Protein Mini Kit (QIAGEN, 80004) and DNAseI digested (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... the soluble fraction containing the His-VitR protein was purified using NTA-resin affinity chromatography in phosphate buffer according to manufacturer’s recommendations (Qiagen). After concentration (Vivaspin 6 Concentrator ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was isolated using the SampleIN Direct PCR Kit (highQu) or the AllPrep DNA/RNA/Protein Mini Kit (Qiagen), and amplified with ALLin HS Red Taq Mastermix (highQu ...
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bioRxiv - Biophysics 2024Quote: ... cleaved protein was filtered using a 0.45 μm syringe filter and purified by a second Ni–NTA purification step (Qiagen). As a final purification step ...
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bioRxiv - Biophysics 2021Quote: The nsp10:16 protein complex was supplemented by 1 mM sinefungin Crystals of the nsp10:nsp16 protein complex grew in three days in commercial JCSG I-IV screens (Qiagen). They were cryo-protected in mother liquor supplemented with 20% glycerol and flash frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the two assay plasmids by temperature-cycle ligation.
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bioRxiv - Cell Biology 2022Quote: ... were performed on significantly altered proteins in CAVIN1-null cells using the “core analysis” function included in the Ingenuity Pathway Analysis (IPA) software (QIAGEN Bioinformatics ...
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bioRxiv - Molecular Biology 2020Quote: RNA and protein were extracted from cell lines using the Qiagen AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA, USA). Total RNA was also DNase treated using the TURBO DNA-free Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: RNA was extracted simultaneously from the same tissue pieces used for DNA extraction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Biochemistry 2021Quote: ... The his-tagged 3C protease was removed by flowing and washing off cleaved transporter protein over two 100 µl beds of Ni-NTA Superflow resin in tandem (Qiagen). Eluent was concentrated using 100 kDa cut-off Amicon Ultra filters (Millipore-Sigma).
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bioRxiv - Microbiology 2021Quote: ... Proteins were purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). Polyclonal rabbit antisera were raised by Covance Research Products Inc ...
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bioRxiv - Biochemistry 2020Quote: Initial crystals of Nup133NTD-VHH-SAN5 were obtained at 18°C in 1 day as part of the Protein Complex suite (Qiagen) in a 96-well sitting drop tray with a reservoir containing 15% PEG MME 2,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Cell debris and protein aggregates were removed by centrifugation and the supernatant was loaded on a Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with lysis buffer and the protein eluted with lysis buffer containing 200 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: Genomic DNA from 1×106 FACS sorted p7 MPs from one animal was isolated with the All Prep DNA / RNA / Protein Mini Kit (Qiagen) and used as one sample ...
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bioRxiv - Biochemistry 2020Quote: ... Cell debris and protein aggregates were removed by centrifugation and the supernatant was loaded on a Ni-NTA Superflow affinity resin (Qiagen). The resin was washed with lysis buffer and the protein eluted with lysis buffer containing 200 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... Genomic DNA of clones showing depletion of spartin protein by immunoblot analysis with the spartin antibody were extracted (DNeasy Blood and Tissue Kit, Qiagen), and the genomic DNA sequence surrounding the target exon of spartin was amplified by PCR (sense ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction containing the protein of interest was obtained by centrifugation at 12000xg for 20 min and was mixed with Ni2+-NTA beads (Qiagen) for 1.5 h by rotation at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed 3 times with sterile 1x PBS and were then lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen). Experiments were performed four times.
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bioRxiv - Immunology 2022Quote: ... Plasmids encoding the recombinant full-length Spike protein and the RBD were provided by F. Krammer (Mt. Sinai) and purified by nickel-nitrilotriacetic acid resin (Qiagen). ELISA plates (Immulon 4 HBX ...
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bioRxiv - Microbiology 2022Quote: ... the His-ChuP protein was purified from the soluble extract by affinity chromatography in a Ni-NTA Superflow column (Qiagen). The elution fractions were evaluated using 18% SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were centrifuged 15,000 x g at 4°C for 30 min and the resulting supernatant containing the target protein was filtered and purified using a pre-equilibrated Ni-NTA beads column (Qiagen). The recombinant protein was eluted using 4 mL of elution buffer (8 mM Na2HPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole (20 mM final) was added to the eluted protein which was subsequently incubated with 5 mL NiNTA resin (Qiagen) at 4 °C overnight with constant inversion ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from AAV1-transduced primary hippocampal neurons (DIV12-14) using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Total RNA was polyA selected and sequenced (Hudson Alpha GSL ...