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Citations for Qiagen :
851 - 900 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The following oligos at the indicated concentration were used: 5nM of miR-455-5p mimic (MSY0003150, Qiagen) or recommended All Stars negative control siRNA (cat ...
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bioRxiv - Developmental Biology 2021Quote: ... combined with treatment with RNase-Free DNase Set (Qiagen). cDNA was generated from purified total RNA using PrimeScript RT Master Mix (Takara Bio) ...
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bioRxiv - Genomics 2020Quote: ... and GeneRead Adapter I set B (Qiagen, cat # 180986) according to Qiagen protocol with one exception ...
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bioRxiv - Biochemistry 2019Quote: ... including digestion with the RNase-Free DNase Set (Qiagen). cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... with two sets of 96-well adapter plates (QIAGEN) containing 2 ml microcentrifuge tubes (QIAGEN ...
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bioRxiv - Cancer Biology 2021Quote: ... we used the same set of two siRNAs (Qiagen) against HNF1B compared with negative and positive control siRNA (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... and DNase I treated (RNase-Free DNase Set, Qiagen). Sample quality was checked using a TapeStation RNA ScreenTape assay (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was eliminated with RNase-Free DNase Set (Qiagen) in solution ...
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bioRxiv - Microbiology 2021Quote: ... or the on-column RNase-free DNase set (QIAGEN). RNA was reverse transcribed using M-MLV reverse transcriptase and random primers (Invitrogen) ...
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bioRxiv - Systems Biology 2022Quote: ... An additional DNaseI treatment (Qiagen RNase-Free DNase Set) was performed on the purified sample in order to remove residual gDNA and re-purified using the RNeasy kit ...
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bioRxiv - Developmental Biology 2022Quote: ... The RNase-Free DNase Set (79254; Qiagen, Hilden, Germany) was used to ensure that there was no DNA contamination ...
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bioRxiv - Microbiology 2021Quote: ... and its associated RNase- Free DNase digestion set (QIAGEN), following the manufacturer’s protocol for mammalian cells ...
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bioRxiv - Genetics 2021Quote: ... or RNeasy kit and RNase-free DNase set (QIAGEN) and Superscript First Strand Synthesis kit (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... After treatment with DNase (RNase-Free DNase Set, Qiagen), RNAs were purified with an RNeasy Mini Elute cleanup kit (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... on-membrane DNase I (RNase-Free DNase Set, Qiagen) digestion was performed according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... and was treated with RNase-Free DNase Set (Qiagen) as described by (Brunner et al ...
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bioRxiv - Plant Biology 2022Quote: ... in combination with the RNase-Free DNase set (QIAGEN). Total RNA was used for first strand cDNA synthesis by the ReverTra Ace qPCR RT Master Mix (Toyobo) ...
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bioRxiv - Neuroscience 2022Quote: ... and RNase- Free DNase set (Qiagen, Cat. no. 79254). Library preparation was conducted by Novogene (https://en.novogene.com/ ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was treated with RNase-free DNase Set (Qiagen) and cDNA was synthesized from 300 ng of total RNA using the M-MLV Reverse Transcriptase (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... in conjunction with the RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... in combination with the RNase-Free DNase Set (QIAGEN). Integrity of RNA samples was evaluated using the RNA 6000 Nano Kit (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... in combination with the RNase-Free DNase Set (QIAGEN). The isolated RNA was loaded on a 15% TBE Urea size selection gel and after staining with SYBR Gold (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... and treated with the RNase-Free DNase Set (Qiagen). 1 µg total RNA was reverse transcribed with oligo dT using Sensifast cDNA Synthesis kit (Meridian Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... in combination with the RNase-Free DNase Set (QIAGEN) according to the manufactureŕs instructions ...
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bioRxiv - Plant Biology 2023Quote: ... including the treatment with RNase-Free DNase Set (QIAGEN).
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bioRxiv - Molecular Biology 2024Quote: ... Following DNAse treatment with RNase-Free DNase Set (Qiagen), RNA concentration and purity were assessed by Nanodrop 2000 spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Neuroscience 2023Quote: ... with DNase digest using RNase-free DNase set (QIAGEN).
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bioRxiv - Molecular Biology 2021Quote: ... 1× QuantiNova RT Mix (Qiagen), 1× QuantiNova SYBR Green RT-PCR Master mix ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized by Qiagen Omniscript RT Kit (#205111, Qiagen). RT-qPCR was performed using QuantiTect SYBR® Green PCR Kit (#204145 ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized by Qiagen Omniscript RT Kit (#205111, Qiagen). RT-qPCR was performed by using QuantiTect SYBR® Green PCR Kit (#204145 ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized by Qiagen Omniscript RT Kit (205111, Qiagen). The relative mRNA level of indicated genes was normalized to that of the internal control Hsp90 and calculated by the equation 2^(Ct(cycle threshold ...
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bioRxiv - Bioengineering 2019Quote: ... Sensiscript RT Kit (QIAGEN, Germany) was used for cDNA synthesis with 50 ng per reaction according to the manufacturers’ instructions ...
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bioRxiv - Genetics 2020Quote: ... with RT² SYBR Green (Qiagen), POWER SYBR (Thermo Fisher) ...
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bioRxiv - Genetics 2022Quote: ... miScript II RT Kit (Qiagen) was used to synthesize cDNA ...
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bioRxiv - Microbiology 2024Quote: ... the Omniscript RT kit (Qiagen) using random hexamers (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... the detection of N-gene of SARS-CoV-2 was performed by using the 2019-nCoV-2 RUO kit (Integrated DNA Technologies, Inc., Coralville, Iowa, USA) and One-Step RT-PCR Kit (QIAGEN® GmbH) on a Rotor-Gene Q real-time PCR cycler (QIAGEN® GmbH) ...
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bioRxiv - Microbiology 2022Quote: ... Correct introduction of the NS1 mutations was verified via cDNA synthesis of viral RNA using the One-Step RT-PCR kit (Qiagen, Hilden, Germany), amplification and sequencing using NS-specific primers ...
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bioRxiv - Immunology 2020Quote: RNA from B1a B cells was reverse transcribed and amplified using a Qiagen OneStep RT-PCR kit (Qiagen Inc., Valencia, CA, USA) and iRepertoire® mouse BCR heavy chain (MBHI ...
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bioRxiv - Microbiology 2020Quote: ... an aliquot of the extracted RNA solution was added to the reaction mixture for the QuantiTect probe RT-PCR kit (Qiagen, Hilden, Germany), which contained 2x QuantiTect probe RT-PCR master mix ...
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bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instruction and the resulting cDNA was used for reverse transcription-polymerase chain reaction (RT-PCR) using HotStarTaq Plus Master Mix Kit (Qiagen, Germantown, MD). The PCR conditions used were as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... RT2 qPCR Primer assays for GAPDH and primers for target genes were respectively purchased from Qiagen and IDT ...
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bioRxiv - Developmental Biology 2019Quote: ... Primers sequences are shown in Extended data table 2 and predesigned quantitech primer assays (Qiagen, 249900) were used for other genes including Gal (QT00109970) ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of 20 mM of each primer (KAN-2 FP1 or KAN-2 RP1 complementary to the Tn5 sequence with the bubble primer 224) and 10 μL of the 10X Qiagen Multiplex PCR Master Mix Kit (Qiagen, Valencia, CA, USA) in a final volume of 100 μL ...
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bioRxiv - Plant Biology 2022Quote: ... All qPCR reactions were performed with primer concentrations at a final concentration of 250 nM in a Rotor-Gene Q real-time PCR cycler (Qiagen, Q-Rex v1.0), using the Rotor-Gene SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1) and miRCURY® LNA® miRNA SYBR® Green PCR (Qiagen). Values were normalized to miRNA quantity and expressed as relative expression to control WS using formulae 2−ΔCT.
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bioRxiv - Genetics 2023Quote: ... 4 μL PCR mastermix (made up of 0.75 μl at a concentration of 0.2 μM of each primer, 80 μl ultrapure water, and 250 μl QIAGEN Multiplex PCR mix; QIAGEN Inc. Cat. No. 20614). PCR conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR reactions were run using a QuantiTect SYBR Green RT-qPCR kit (Qiagen, 204243) on a Qiagen Rotorgene Q ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR reactions were run using the QuantiTect SYBR Green RT-qPCR kit (Qiagen, 204243) on a Qiagen Rotor-Gene Q ...
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bioRxiv - Cancer Biology 2021Quote: ... using predesigned Quanititect Primer assays (Qiagen) to the following murine genes ...