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Citations for Qiagen :
801 - 850 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was isolated using the miRNeasy Micro Kit from Qiagen and the RNase-free DNase set from Qiagen according to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2024Quote: ... and purified using RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... following DNA digestion (RNase-free DNase set, Qiagen) and by using a Qiacube instrument ...
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bioRxiv - Immunology 2023Quote: ... with the RNase-Free DNase Set (Qiagen #79254) to remove any residual DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... and the RNase-Free DNase Set (Qiagen, 79254) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... including the DNase (RNase-Free DNase set, Qiagen) treatment to remove residual DNA ...
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bioRxiv - Genetics 2020Quote: ... 1μl of bisulfite converted DNA was used as template to amplify target genomic regions using specific primers (one biotinylated) with the PyroMark PCR kit (Qiagen, #978703), following the recommended conditions (annealing temperature 56°C) ...
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bioRxiv - Microbiology 2019Quote: ... the 16S rRNA gene V4 variable region PCR primers 515/806 were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... assay targeting the paramyxovirus L protein gene was adapted using primers developed by Tong et al (36) and the Qiagen AllTaq PCR Core kit (QIAGEN, Australia). Amplicons corresponding to the expected size (584 bp ...
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bioRxiv - Biophysics 2021Quote: ... A small volume of this reaction was then used to seed a PCR reaction using primers prAH869 and prAH870 in Q5 High-Fidelity 2X Master Mix (Qiagen M0492). Products were purified by RNAClean XP magnetic beads (Beckman-Coulter A63987 ...
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bioRxiv - Microbiology 2020Quote: ... PCR replicates were pooled and remaining primers were enzymatically digested as indicated above and purified using QIAquick PCR Purification Kit (Qiagen, Germany). PCR-products were quantified (NanoDrop 2000c ...
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bioRxiv - Cancer Biology 2022Quote: ... Synthesis of cDNA and qRT-PCR were performed as previously described (50) using the following primers (all from Qiagen, Limberg, Netherlands): ATF4 (QT00074466) ...
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bioRxiv - Neuroscience 2022Quote: ... Five μl of reverse transcribed cDNA samples were pre-amplified using RT2 preamp PCR master mix with PBR-152Z RT2 preamp pathway primer mix (Qiagen, Inc.) on a thermal cycler under the following conditions ...
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bioRxiv - Neuroscience 2023Quote: ... A single amplicon of 209 bp (chr6:32063788-32063996) was PCR-amplified using primers designed with the PyroMark Assay Design software 2.0 (Qiagen, Hilden, Germany) (Supplementary Table 10) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ten µl PCRs containing 1 µl of template DNA (10–50 ng of DNA from each fire coral sample) and 0.25 µM of each primer were run in triplicate per sample using the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Thermal cycler conditions for ITS2 PCR amplification were 95°C for 15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cy3-labeled miR-430 was separated from unconjugated dyes using a QIAquick Nucleotide Removal Kit (QIAGEN).
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bioRxiv - Genomics 2022Quote: ... DE cells were treated with 500 nM LNA miR-182 inhibitor (Qiagen, Hilden, Germany; #4101243-101) or scrambled control (Power Negative Control A ...
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bioRxiv - Physiology 2022Quote: ... we spiked in miR-39 as an exogenous housekeeping miRNA control prior to extraction (Qiagen #219610). TaqMan primers were obtained from Life Technologies for miRNAs 106b 5p (#000442) ...
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bioRxiv - Neuroscience 2024Quote: ... mHypoE-N46 cells were transfected with a miR-505-5p mimic (miRCURY LNA miRNA mimic, Qiagen) or negative control ...
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bioRxiv - Neuroscience 2023Quote: ... Retro transcription (RT) was performed using the miRCURY LNA RT kit (Qiagen, Germany).
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bioRxiv - Systems Biology 2019Quote: ... Primers were purchased from Qiagen (il1β ...
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bioRxiv - Immunology 2020Quote: ... Primers were from QuantiTect (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... and using primers from Qiagen targeting Per1 (QT00113337) ...
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bioRxiv - Neuroscience 2019Quote: ... Primers were purchased from Qiagen or Eurofins Genomics and designed according to GenBank sequence information at the National Center for Biotechnology Information (NCBI ...
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bioRxiv - Neuroscience 2021Quote: ... QuantiTect primer assays (Qiagen, Germany) for selected genes were used ...
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bioRxiv - Cell Biology 2020Quote: ... Human RPLP0 (primers from QIAGEN) was used as reference gene for cDNA from Caco-2 samples ...
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bioRxiv - Microbiology 2019Quote: ... Primers for respective miRNA (Qiagen) were used in qRT-PCR reactions which are as follows:
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bioRxiv - Pathology 2020Quote: ... and miScript primer assay (Qiagen) were used for semi-quantitative determination of the expression of mouse miR-19b-1 (MS00005915) ...
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bioRxiv - Cell Biology 2021Quote: ... Hprt primers were from QIAGEN, the other primer sequences are indicated in Supplementary Table IV.
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bioRxiv - Neuroscience 2021Quote: ... Gapdh (QuantiTect Primer Assays; Qiagen). All samples were run in triplicate ...
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bioRxiv - Cancer Biology 2021Quote: ... and QuantiTect qPCR primers (Qiagen). Each reaction contained 10 ng of cDNA and 250 nM forward and reverse primers ...
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bioRxiv - Cancer Biology 2023Quote: ... with Quantitect human primers (Qiagen) for genes ID1 ...
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bioRxiv - Immunology 2022Quote: ... Only validated primers from Qiagen were used.
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bioRxiv - Physiology 2023Quote: ... QuantiTecht Primer Assays (Qiagen, Germany) were utilized for Pdgfb (QT00266910 ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR, and immunoblotting. For knock-down experiments, non-specific (siNS, No. 1027310) or AHR (siAHR, SI02780148) siRNA (QIAGEN, Inc.) was transfected with LipofectamineTM RNAiMAX (Thermo Fisher Scientific Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed in an Applied Biosystems QuantStudio 3 thermocycler using the QuantiNova Probe SYBR Green PCR Kit (Qiagen, Germany); mix proportions and cycling parameters were used as described in manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the variable region genes from each single cell were amplified in a reverse transcriptase polymerase chain reaction (RT-PCR: QIAGEN, Germany) using a cocktail of sense primers specific for the leader region and antisense primers to the Cγ constant region for heavy chain and Cκ and Cγ for light chain ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng RNA were reverse transcribed using the QIAGEN OneStep RT-PCR Kit according to the manufacturer’s information (Qiagen, Hilden, Germany). The sequences of primers were as follows ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reaction was performed on yield cDNA synthetized from each sample using QuantiTec® Rotor-GeneTM SYBR Green RT-PCR kit (Qiagen) using the Qiagility instrument software (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... RT-PCR products were gel purified in 3% agarose gel and cleaned up using QIAGen gel extraction kit (QiaGen, Hilden, Germany).
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bioRxiv - Cell Biology 2023Quote: ... One μg of RNA with its genomic DNA ablated was converted into cDNA as instructed by the QIAGEN OneStep RT-PCR Kit (Qiagen, UK). Twenty ng cDNA (relative to RNA amount ...
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bioRxiv - Cancer Biology 2023Quote: RT PCR: Total RNA was eluted from knockdown and control cells using the RNeasy® Plus mini kit (Qiagen, Ref # 74134). 2 µg of total RNA was reverse transcribed using High Capacity cDNA Reverse Transcription Kit (AppliedBiosystems ...
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bioRxiv - Immunology 2023Quote: ... RT-PCR for PI3K mRNA was performed using the QuantiTect® Probe RT PCR Kit and the double-stranded DNA stain SYBR green according to the manufacturer’s instructions (Qiagen, ThermoFisher). B-actin was used as the control ...
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bioRxiv - Microbiology 2023Quote: ... RT PCR was performed on a Roche LightCycler 96 using the The RT² Profiler™ PCR Array Human Cell Death PathwayFinder (PAHS-212Z, Qiagen)w ...
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bioRxiv - Immunology 2022Quote: ... qPCR was performed using the Mx3000/Mx3005P Real-Time PCR System. All primers were used in this study were ordered from Qiagen BCAM (PPH12956A), SCGB1A1 (PPH01032F) ...
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bioRxiv - Neuroscience 2021Quote: ... The gene primer for il-1α was a QuantiTect Primer Assay (Cat. No. QT00113505; Qiagen). The sequences for the remaining gene primers can be found in Table 1 and were ordered through Integrated DNA Technologies and diluted to 0.13 μM to be used for PCR ...
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bioRxiv - Cancer Biology 2020Quote: ... Expression of PAINT was determined using PAINT-specific primer pairs (RT2 qPCR Primer Assays - Qiagen) and internal control EIF3D and RPL13A specific primers (RT2 qPCR Primer Assays -Qiagen ...
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bioRxiv - Immunology 2020Quote: ... Gene specific primers for IL-6 (Hs_IL6_1_SG QuantiTect Primer Assay) was sourced from QuantiTect (Qiagen) primers ...
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bioRxiv - Neuroscience 2020Quote: ... and miR-9/9*-124 + shPTBP2 using TRIzol Reagent in combination with RNeasy mini kit (Qiagen, 74104). RNA quality (RIN > 9.6 ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 nM of a miR-96-5p inhibitor or 100nM of a scrambled inhibitor (control) (Qiagen, UK).