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Citations for Qiagen :
1101 - 1150 of 5919 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and genomic DNA was digested with RNase-free DNase set (Qiagen, 79254). miRNA reverse transcription was performed using miRCURY LNA reverse transcription kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA was digested with DNase I (RNase-Free DNase Set, Qiagen). Concentration and purity of RNA were both evaluated with NanoDrop spectrophotometer ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 µg RNA was DNase treated using RNase-Free DNase Set (Qiagen). 1 µg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... with on-column DNase digestion with the RNase-free DNase Set (Qiagen). RNA concentration and purity were evaluated using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNeasy Mini Kit and RNase-free DNase set were from Qiagen. High-Capacity cDNA Reverse Transcription Kit was from Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... Purified RNA was inbcubated with DNase I (RNase-Free DNase Set, Qiagen) over a mini kit column (RNAeasy ...
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bioRxiv - Cell Biology 2021Quote: ... 5 μg RNA was DNase treated using RNase-Free DNase Set (Qiagen) and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2021Quote: ... with on-column DNase digestion with the RNase-Free DNase Set (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA samples were then treated with the RNase-free DNase set (Qiagen) to remove gDNA contaminants and QC checked using Qubit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... The extracted RNA was treated with RNase-free DNase set (Qiagen, USA) to remove residual genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... On-column DNase I treatment using the RNase-free DNase set (Qiagen) was included in the RNA extraction to remove any DNA contaminants ...
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bioRxiv - Molecular Biology 2022Quote: ... The resultant RNA was then treated with RNase-free DNase Set (QIAGEN) according to the protocol provided by the manufacturer with some minor modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... On-column DNAse treatment was performed using Qiagen DNAse Set (Qiagen #79254) at room temperature for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... with an on-column DNase digestion using RNAse-free DNase Set (Qiagen) for 30 minutes to eliminate genomic DNA contamination ...
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bioRxiv - Neuroscience 2022Quote: ... and genomic DNA was removed using the RNase-Free DNase Set (QIAGEN). Retrotranscribed cDNA was obtained from 1 μg of total RNA using the SuperScript VILO Retrotranscription kit from Life Technologies according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254). cDNA was synthesized with random hexamers from the Superscript III First-Strand Synthesis System (Invitrogen 18080051) ...
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bioRxiv - Microbiology 2023Quote: ... The siRNAs were selected from the FlexiTube GeneSolution 4 siRNA sets (Qiagen) and transfected as a mix at 24 nM in Calu-3 and 10 nM in Caco-2 following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... DNase treatment was performed on-column using RNase-Free DNase Set (QIAGEN). cDNA was generated from 200ng-1ug RNA by using SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme Kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was removed from samples using the RNase-free DNase Set (QIAGEN). RNA quantity and quality was assessed using the Agilent 2100 Bioanalyser in conjuction with the RNA 6000 PicoLabChip Kit following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and on-column DNase digestion with the RNAse-Free DNase Set (QIAGEN). Purity and concentration of the extracted RNA were measured using Nano-Drop lite Spectrometer (Thermo-Fisher ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and on-column DNA digestion with the RNase-free DNase set (Qiagen). In order to remove any remaining DNA contamination ...
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bioRxiv - Plant Biology 2023Quote: ... followed by DNase digestion with RNase free DNase Set (Qiagen, Hilden, Germany). RNA samples were quantified on Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... with on-column DNA digestion using RNase-Free DNase Set (Qiagen, Germany). Purified RNA was quantified using QubitTM RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... including digestion of contaminating DNA using an RNase-free DNase set (QIAGEN). RNA was diluted from the columns in ultrapure distilled water ...
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bioRxiv - Genetics 2023Quote: ... DNA contamination was further removed by the RNase-Free DNase Set (QIAGEN). RNA libraries were prepared with Illumina TruSeq Stranded mRNA (poly-A selection ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were treated with RNase Free DNase Set (Qiagen. The Netherlands) to remove genomic DNA contamination ...
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bioRxiv - Molecular Biology 2024Quote: ... The extracted RNA samples were treated with RNase-free DNase set (Qiagen). For RNA-seq ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was digested on column using RNase-Free DNase Set (Qiagen 79254). RNA was reverse transcribed using High-Capacity cDNA Reverse Transcription kit with RNase Inhibitor (Applied Biosystems 4374966 ...
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bioRxiv - Cancer Biology 2024Quote: Differentially expressed gene sets were analyzed using Ingenuity Pathway Analysis Software (Qiagen). Gene sets were uploaded into IPA for Core Expression Analysis of expression data ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The human U6 primer used in this experiment was included in the miScript Primer assay kit (Qiagen Inc., Germantown, MD, #218300).
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bioRxiv - Immunology 2021Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S1) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
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bioRxiv - Immunology 2023Quote: ... 3.5 μl of cDNA was used as template and amplified in a total volume of 40 μl with a mix of forward L-VH primers (Table S3) and reverse Cγ primer and using the HotStar® Taq DNA polymerase (Qiagen) and 50 cycles of PCR (94°C 30 s ...
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bioRxiv - Molecular Biology 2019Quote: Reverse transcription (RT) of total RNA to cDNA was performed in 50 μL reaction volumes using the miRCURY RT kit (Qiagen) and 10 μL total RNA as input ...
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bioRxiv - Cancer Biology 2023Quote: ... was reverse transcribed into cDNA by miScript RT II Kit using miScript HiSpec Buffer or miRCURY LNA RT Kit (Qiagen). Expression levels of miR-142-5p were analyzed using miScript PCR System or miRCURY LNA SYBR Green PCR Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... Two μL of isolated RNA were used for reverse transcription (RT) and cDNA synthesis using the miRCURY LNA RT Kit (Qiagen) according to the manufacturer’s instructions adding the RNA spike-in UniSp6 as quality control for RT ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with Omniscript RT Kit (Qiagen). The relative mRNA expression level of each target gene was quantified by qPCR using KAPA SYBR fast qPCR master mix (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Microbiology 2020Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Microbiology 2021Quote: ... in one-step probe RT-qPCR kits (Qiagen) with the following cycle conditions ...
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bioRxiv - Cell Biology 2021Quote: ... or miRscript RT kit II (Qiagen, Ref. 218161), respectively ...
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bioRxiv - Immunology 2021Quote: ... reverse transcribed into cDNA (QuantiTect RT kit, Qiagen) and analyzed by RT-qPCR.
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bioRxiv - Immunology 2023Quote: ... and Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.45 μL of OneStep RT Mix (Qiagen, Germany), 8.4 μL of HDPCR Mix ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using Sensiscript RT Kit (QIAGEN) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... and the miRCURY LNA RT Kit (Qiagen, UK) was used to conduct qPCR in the Roche ® LightCycler® 480 according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Following seeding MSCs were transfected with 20nM of either pBAE:miR-29b (miR-29b) complexes or pBAE:siRNA (Scramble) complexes (Qiagen, AllStars Negative Control siRNA) or cultured in standard control medium (control ...
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bioRxiv - Cancer Biology 2023Quote: 1×104 SiHa or 1×103 Hela cells/6-well were seeded and transfected with pSG5-miR-142-5p expression vector or pSG5 empty vector as a control using PolyFect Transfection Reagent (Qiagen, Hilden, Germany). After 48 h ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was removed using an RNase-free DNase set (Cat# 79254, Qiagen). The cDNA was synthesized via treatment with reverse transcriptase and oligo(dT ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting gene sets were analysed using Ingenuity Pathway Analysis (IPA) software (Qiagen) (Kramer et al. ...