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Citations for Qiagen :
601 - 650 of 6247 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Human primers were pre-validated Quantitect primers (Qiagen, Manchester, UK). Comparative quantification normalised target gene mRNA to β-actin (ACTB ...
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bioRxiv - Cell Biology 2021Quote: ... Validated qPCR primers and primer assays (Qiagen, see Table 1) were used together with QuantiTect SYBR Green PCR kit (QIAGEN ...
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bioRxiv - Plant Biology 2020Quote: ... An RNase-Free DNase Set (Qiagen) was used to remove genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... with RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... with RNase-Free DNase Set (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Individual set of two siRNAs (Qiagen) against HNF1B or ERG were tested in knockdown efficiency and compared with the siRNA negative-control (Qiagen ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and RNase- Free DNase Set (Qiagen). RNA integrity numbers (RIN ...
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bioRxiv - Immunology 2022Quote: ... and RNase-Free DNase Set (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNase-free DNase Set (Qiagen) was used for on-column DNase digestion ...
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bioRxiv - Genetics 2019Quote: ... and RNase-Free DNase Set (QIAGEN). Adaptors as well as low quality base pairs were trimmed ...
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bioRxiv - Microbiology 2021Quote: ... The RNase-Free DNase Set (Qiagen) treatment was applied to all samples according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNase-free DNase Set (Qiagen). Infection was confirmed by RT-qPCR using specific primers to detect HDV transcripts.
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bioRxiv - Systems Biology 2023Quote: ... and RNase-Free DNase Set (QIAGEN) according to the product instructions ...
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bioRxiv - Immunology 2023Quote: ... with Rnase-Free DNase set (Qiagen). The RNA was eluted with RNAse-free H2O in final volume of 20μl and stored at -80°C.
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bioRxiv - Cell Biology 2023Quote: ... and RNase-Free DNAse Set (Qiagen). Total RNA (150 ng ...
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bioRxiv - Immunology 2023Quote: ... including RNase-Free DNase Set (Qiagen) treatment ...
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bioRxiv - Immunology 2023Quote: ... and RNase-Free DNase Set (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... including RNase-Free DNase Set (Qiagen) treatment ...
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bioRxiv - Microbiology 2023Quote: ... and RNase-free DNase Set (Qiagen) on-column DNA digestion ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and RNase-Free DNase Set (QIAGEN) according to product instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNase-free DNase Set (Qiagen) on-column DNA digestion ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1.0–3.0 μl (5.0–15 pmol) of primers and 7.5–15 μl of 2x Multiplex PCR Plus Master mix (QIAGEN). The PCR protocol consisted of an initial DNA polymerase (HotStar Taq ...
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bioRxiv - Cancer Biology 2020Quote: ... 1μl of forward/reverse primers (5pmol) and 3μl of nuclease-free water were mixed in a 0.1mL real-time PCR (Qiagen) to conduct PCR in a 10μl of reaction volume ...
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bioRxiv - Genomics 2022Quote: ... and then PCR-amplified with biotinylated primers and pyrosequenced for allele quantification on a Pyromark Q24 system (Qiagen). The same PCR was done on no-reverse transcription control samples to confirm absence of genomic DNA contamination ...
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bioRxiv - Immunology 2020Quote: ... Primers for PCR amplification and sequencing were designed with PyroMark® Assay Design 2.0 software (QIAGEN, Hilden, Germany). PCRs were performed with the IMMOLASE™ DNA polymerase PCR kit (Bioline) ...
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bioRxiv - Cancer Biology 2022Quote: PCR primers for pyrosequencing methylation analysis were designed using the Pyromark Assay Design SW 1.0 software (Qiagen, Germany) (Table 1) ...
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bioRxiv - Microbiology 2022Quote: ... and sgRNA-specific and universal primers (Table 1) and subsequently purified with the QIAquick PCR Purification Kit (Qiagen). sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre) ...
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bioRxiv - Genetics 2024Quote: ... 10μl of PCR-product and 2µl of sequencing primer (4μM) were then pyrosequenced on a Pyromark Q48 Autoprep (Qiagen), as described before 22 ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR primers for the Prkcz locus were designed using the Pyromark® Assay Design SW 2.0 software (Qiagen). In brief ...
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bioRxiv - Immunology 2024Quote: ... Libraries were rendered using the barcoded primers Ad1_noMX as forward and Ad2.1-6 as reverse and purified using a PCR cleanup kit (Qiagen), yielding a final concentration of about 30 nM in 20 μL ...
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bioRxiv - Cancer Biology 2023Quote: CXCL5 primer (Qiagen Mm_Cxcl5_2_SG QuantiTect Primer Assay QT01658146 ...
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bioRxiv - Cancer Biology 2023Quote: ... GAPDH primer (Qiagen Mm_Gapdh_3_SG QuantiTect Primer Assay QT01658692)
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bioRxiv - Immunology 2023Quote: ... universal primer (Qiagen) and SYBR Green (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... FS RNAs and cellular GAPDH pre-mRNAs were done with the QuantiTect SYBR Green RT-PCR Kit (Qiagen), and performed essentially as described previously (56 ...
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bioRxiv - Microbiology 2021Quote: ... Assays were run in a 384-well format using the QuantiFast Pathogen RT-PCR + IC Kit (QIAGEN; 211454). USA/WA-1/2020 SARS-CoV-2 RNA (20,000 genome copies per reaction ...
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bioRxiv - Bioengineering 2020Quote: ... RT-PCR amplifications were carried out using a Power SYBR Green RNA-to-CT 1 step kit (Qiagen) on a ViiATM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantification of gene expression level was conducted with Rotor-Gene SYBR Green RT-PCR kit (Qiagen, cat# 204174) on the Rotor-Gene-Q PCR cycler (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: The quantitative real-time TaqMan based assay was carried out using a One-step RT-PCR kit (Qiagen) in the Light Cycler 2.0 system (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR amplifications were carried out using Power SYBR Green RNA-to-CT 1 step kit (Qiagen, UK) on a 384 well ViiA Real-Time PCR System (Applied Bio-systems ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed in an Applied Biosystems QuantStudio 3 using the QuantiTect® Multiplex PCR Kit (QIAGEN). The primers and probes used are listed in Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments ranging in size between 150 and 250 base pairs were gel purified using MinElute PCR Purification Kit (QIAGEN). Concentration of the final DNA samples was measured using Qubit™ dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... All primers were purchased from miScript Primer Assays (Qiagen, Valencia, CA) and GAPDH mRNA expression of each sample was used as normalization.
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bioRxiv - Systems Biology 2019Quote: ... set to collection mode range 125-160bp) to extract the library molecules followed by a concentration step (Qiagen MinElute PCR Purification,. 28004) to produce libraries ready for sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... 3.5ul of miRNeasy Serum/Plasma Spike-In Control (miR-39, 1.6 × 108 copies; Qiagen) was added to each CSF sample ...
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bioRxiv - Genomics 2021Quote: ... The anti-sense miRCURY LNA miRNA detection probe dre-miR-146b-5p (YD00613622, QIAGEN) was used ...
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bioRxiv - Immunology 2023Quote: ... Primers were designed using the NCBI primer-designing tool (see Table 1) or ordered as validated primers from Qiagen. The mRNA expression levels were plotted as relative gene expression to β-actin (2-ΔCt) ...
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bioRxiv - Cancer Biology 2021Quote: ... For RT (Qiagen) for miRNA isolation quality control ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 µL RLT buffer (Qiagen) with 1:10 β-mercaptoethanol (55mM ...
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bioRxiv - Genetics 2021Quote: ... The 10 μL reaction was carried out with 40 ng genomic DNA and 0.2 μM of each primer and 5 mM Multiplex PCR Kit (Qiagen). Cycling conditions were identical for both directions with 95°C 15:30 min ...
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bioRxiv - Microbiology 2020Quote: ... which is performed using LRT forward and reverse primers and probe using the Rotor Gene Probe PCR kit (Qiagen) as described above ...