Labshake search
Citations for Covaris :
1 - 50 of 1249 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Sheared to 500 bp length using the E220 Focused-ultrasonicator (Covaris, Woburn, MA, USA) and prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
-
bioRxiv - Cancer Biology 2024Quote: ... Chromatin was sheared to ∼250 bp fragments using a M220 Focused-ultrasonicator (Covaris). For immunoprecipitation ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... and 1500 ng of total DNA was then sheared using Covaris (Covaris, Inc.), or Qsonica (Qsonica LLC ...
-
bioRxiv - Cell Biology 2024Quote: ... sonicated to obtain DNA fragments below 1000 bp in length (Covaris S220 sonicator ...
-
bioRxiv - Microbiology 2024Quote: ... The DNA was fragmented with S220 Focused-ultrasonicators (Covaris, USA) and purified with Agencourt AMPure XP magnetic beads (Beckman Coulter Co. ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1 μg of gDNA was sonicated on an E220 sonicator (Covaris; settings: 17Q, 75s), and libraries were prepared by standard procedure (end repair ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was diluted to 3 µg/130 µL in microTUBEs (Covaris #520045) and sheared in triplicate to 300 bp fragments using the following settings on a Covaris LE220 Focused-Ultrasonicator ...
-
bioRxiv - Neuroscience 2024Quote: ... was taken to the Duke University Center for Genomic and Computational Biology (GCB) and sheared to achieve 175 bp-long fragments using Covaris S220 (Covaris, Woburn, MA). DNA quality was assessed using Agilent 4200 TapeStation ...
-
bioRxiv - Molecular Biology 2024Quote: ... The DNA was then sheared to 200-300 bp with a Covaris M220 focused ultrasonicator (130 uL tube [Covaris part number 520045] ...
-
bioRxiv - Molecular Biology 2024Quote: ... The extracted DNA was sheared to a length of ∼175 bp by sonication (ME220, Covaris) and biotinylated DNA fragments were purified using streptavidin beads (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... the gDNA was sheared into ∼ 200-bp fragments using an S220 Focused-ultrasonicator (Covaris) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: ... DNA was sonicated to a 100–800 bp size range using a Covaris E220 sonicator (Covaris, Woburn, MA, USA), end-repaired and 3′-adenylated ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Extracted DNA was then sheared and enriched into 350 bp fragments using ultrasonication (Covaris S220, Woburn, MA), which were used to construct a genomic library using the TruSeq DNA PCR-free library kit (Illumina ...
-
bioRxiv - Cell Biology 2024Quote: ... 75 W PIP (peak incident factor) and 200 cycles/burst using the M220 focused-ultrasonicator (#500295, Covaris, US). The chromatin was collected by centrifugation at 16000 g for 15 min at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... Supplemental Table S2) were sheared in g-tubes (Covaris) to an average of 50 kb length ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... extracts were sonicated with a M220 Focused-ultrasonicator™ with microTUBEs AFA Fiber Pre-Slit Snap-Cap (Covaris, Woburn, Massachusetts, USA) following the manufacturer’s protocol to generate ∼350 bp insert sizes.
-
bioRxiv - Evolutionary Biology 2024Quote: ... HMW gDNA was sheared into 20kb fragments using g-TUBE (Covaris Inc).
-
bioRxiv - Evolutionary Biology 2024Quote: ... nuclei were extracted and chromatin was sheared using a water-bath sonicator (Covaris E220 evolution ultra-sonicator). Immunoprecipitation was done using the following anti-H3K27ac (Abcam ...
-
bioRxiv - Genomics 2024Quote: 120 μl of DNA sample of 10 μg DNA was transferred to a g-tube (Covaris Part No. 520079). The tube was then allowed to proceed 6 passes of centrifugation with 2,000 x g of 2 minutes each ...
-
bioRxiv - Genetics 2024Quote: ... Genomic DNA was fragmented to 300-400bp using ultrasonication (Covaris S220). Fragmented DNA was used to produce barcoded DNA libraries using NEXTflex™ DNA Barcodes (Bioo Scientific ...
-
bioRxiv - Genetics 2024Quote: ... of purified DNA were fragmented to ∼350bp using the Covaris S2 system (10% duty cycle, intensity 5 for 2x 45 sec in Covaris AFA tubes) followed by a concentration step with Zymo columns (DNA Clean & Concentrator) ...
-
bioRxiv - Genomics 2024Quote: ... or an E220 Focused-Ultrasonicator (Covaris) to an average size of 200-600 bp and the cleared chromatin was used for immunoprecipitation overnight at 4°C with Protein A Dynabeads that were pre-coupled in 0.5% BSA with IgG ...
-
bioRxiv - Developmental Biology 2024Quote: ... SDS was added to the final concentration of 1% and released chromatin solution was then transferred to microtubes with AFA fiber (Covaris, 520045) and sonicated for 14 cycles of 130 second each with peak incident power 70 and duty factor 20 in a Covaris ME220 focused ultrasonicator ...
-
bioRxiv - Genetics 2024Quote: ... The purified DNA samples were fragmented to ∼300 bp with M220 Focused-ultrasonicator (Covaris) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2024Quote: Approximately 4.4 μg of HMW DNA was processed with DNA shearing through 6 passes of centrifugation in a g-tube (Covaris Part No. 520079) at 2,000 x g for 2 min ...
-
bioRxiv - Genomics 2024Quote: ... S3 and S4 (from both 10kh- and 4f-SAMMY-seq) fractions were transferred to screw cap microTUBEs (Covaris, 004078) and sonicated in a Covaris M220 focused-ultrasonicator to obtain a smear of DNA fragments peaking at 200bp (settings ...
-
bioRxiv - Genomics 2024Quote: DNA samples were sheared to an average size of ∼ 15 kb using the G-tube protocol (Covaris, MA). DNA libraries were prepared using a SMRTbell express template prep kit 2.0 (100-938-900 ...
-
bioRxiv - Genomics 2024Quote: ... nuclei were resuspended in 1 mL LB3 and transferred to a 1 mL milliTUBE (Covaris) for shearing ...
-
bioRxiv - Microbiology 2024Quote: The DNA samples were fragmented using the S220 Focused-ultrasonicator (Covaris) to an mean size of approximately 300 bp (Duty Factor ...
-
bioRxiv - Genomics 2024Quote: ... setosum DNA in 120 μl elution buffer was transferred to a g-tube (Covaris Part No. 520079). The sample was then proceeded to 6 passes of centrifugation with 2,000 xg for 2 min ...
-
bioRxiv - Genomics 2024Quote: DNA shearing was first performed from 5 µg HMW DNA in 120 µL elution buffer using a g-tube (Covaris Part No. 520079) with 6 passes of centrifugation at 1,990 x g for 2 min ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was fragmented into 2–8 kb pieces using focused ultrasonication (Covaris E220 with miniTUBE red). DNA Fragments were subsequently cloned into a PCR-linearized pZE21 expression vector (primers 1 and 2 ...
-
bioRxiv - Microbiology 2024Quote: ... Ultrasonic random shearing (Covaris M220) was performed to generate fragments with lengths ≤800 bp ...
-
bioRxiv - Microbiology 2024Quote: ... The total DNA for each sample was fragmented using the ultrasonicator Covaris S220 (Covaris Inc.), and the fragments were separated by size in a Pippin Prep electrophoretic system (Sage Science ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified DNA was further fragmented to the size of ∼150 bp by the Covaris focused-ultrasonicator (Covaris M220) and subjected to library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Genetics 2024Quote: ... 100 ng of genomic DNA from each sample was sheared using a Covaris M220 (300bp settings, Covaris). Libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (Catalogue Number ...
-
bioRxiv - Genomics 2024Quote: ... a dilution of 5 µg HMW DNA in 120 µL elution buffer was transferred to a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation with 1,990 x g for 2 min ...
-
bioRxiv - Genomics 2024Quote: 120 μl of DNA sample with around 8-10 μg DNA was transferred to a g-tube (Covaris Part No. 520079). The sample was then proceeded to 6 passes of centrifugation with 2,000 xg of 2 min ...
-
bioRxiv - Plant Biology 2024Quote: The qualified DNA was sheared with a g-tube (Covaris Part No. 520079) for 6 passes of centrifugation at 1,990 x g for 2 min and was subsequently purified with SMRTbell® cleanup beads (PacBio Ref ...
-
bioRxiv - Cancer Biology 2024Quote: ... The 300ng genomic DNA concentrations were sheared with Covaris LE220 Sonicator (Covaris) to target of 150-200bp average size ...
-
bioRxiv - Cancer Biology 2024Quote: Macrodissected DNA samples were sheared on a Covaris S2 Focus Ultrasonicator (Covaris, USA) to a target length of 250-350 bases ...
-
bioRxiv - Plant Biology 2024Quote: ... the HMW DNA was fragmented with g-tubes (Covaris) to get 20 kbp fragments and then a library was prepared according to the recommendations of the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences) ...
-
bioRxiv - Systems Biology 2024Quote: ... DNA was sheared using an ultrasonicator (Covaris) prior to tagmentation ...
-
bioRxiv - Microbiology 2024Quote: ... 100 ng of genomic DNA was fragmented to 300 bp by ultrasonication (S220 Covaris, Duty Factor ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 200 bp using a LE220 sonicator (Covaris, Bankstown, Australia). After sonication ...
-
bioRxiv - Immunology 2024Quote: ... 0.5% SDS) with 1/200 PIC and transferred into Covaris tubes (Covaris, 520045). Samples were sonicated for 180 seconds using the settings aiming for 200 bp fragments ...
-
bioRxiv - Genomics 2024Quote: ... using Covaris E220 Focused-ultrasonicators (Covaris) for 14 cycles ...
-
bioRxiv - Cell Biology 2024Quote: ... in 130 μL 10mM Tris-HCl pH 8.0 was fragmented using a Covaris E220 focused ultrasonicator in a microTUBE AFA Fiber Snap-Cap (Covaris, 520045) with the Duty Factor set to 10 and Peak Incident Power set to 175W at 200 cycles per burst for 180 seconds per sample ...
-
The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... nuclei were resuspended in 1mL of “Shearing Buffer” and were sonicated with a S220 Focused-ultrasonicator (Covaris) (Power 140W ...
-
bioRxiv - Synthetic Biology 2024Quote: ... qualified DNA samples were randomly broken into 350 – 500 bp fragments by Covaris. After library construction ...