Labshake search
Citations for Covaris :
351 - 400 of 1249 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 1 µg of genomic DNA samples were fragmented using g-tubes (Covaris). Sheared DNA was purified with AMPure PB beads and was used to construct a SMRTbell library according to the PacBio library construction guidelines59 ...
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bioRxiv - Genetics 2023Quote: ... Input gDNA was fragmented by Covaris S220 or LE220 Focused-ultrasonicator to an average insert size of 350bp ...
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bioRxiv - Genomics 2023Quote: ... approximately 200 ng of DNA was fragmented by Covaris M220 to generate a mean fragment distribution of 350bp ...
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bioRxiv - Genomics 2023Quote: ... Using a gTUBE (Covaris) we sheared 10 µg of HMW DNA to an average fragment size of of 24 kb and prepared two independent non-barcoded HiFi SMRTbell libraries using SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Genomics 2023Quote: ... genomic DNA was disrupted randomly with 6 kb-20 kb fragments by g-TUBE (Covaris, Woburn, MA, USA) and sequenced by the PacBio Sequel platform ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were prepared using truChIP Chromatin Shearing Reagent Kit (Covaris).
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bioRxiv - Molecular Biology 2023Quote: ... 3 times 30 seconds ON/OFF sonication was carried out at high intensity (Covaris S2 Focus Ultrasonicator) to disrupt the chromatin and release nuclear proteins ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysis was performed with a Tissue Homogenizer (Covaris). Subsequent tryptic digestion ...
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bioRxiv - Plant Biology 2023Quote: ... Young leaves from a single 3-week-old plant were collected for high-molecular-weight DNA extraction followed by shearing to fragments with an average size of 20 kb using g-TUBE (Covaris). The sheared DNA was then used to construct a PacBio SMRT library following the standard SMRT bell construction protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 1.0 μg of genomic DNA from proband’s blood is fragmented to an average length of 140 bp using focused acoustic energy (Covaris E210). DNA fragments were subjected to exome capturing using the IDF xGen capture probe panel with an additional “spike-in” of ∼2,500 region which are total 620 kb of RefGene coding regions ...
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bioRxiv - Cancer Biology 2023Quote: ... and chromatin was isolated and fragmented by sonication (Covaris). Fragmented chromatin was loaded onto REST antibody (Millipore ...
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bioRxiv - Genomics 2023Quote: ... We cryo-pulverised the tissues into a rough powder using a cryoPREP (Covaris) automated dry pulverizer (3 impacts to an extra thick TT1 TissueTube on intensity setting 6 with immersion in liquid N2 for 10 seconds between impacts) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was fragmented by sonic disruption using a Covaris S220 apparatus (Covaris). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... fibrils were thawed and sonicated in a sonicator water bath to generate PFFs (Covaris S220 ...
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bioRxiv - Developmental Biology 2023Quote: ... Suspensions were aliquoted into S2 microfibre tubes (Covaris). Suspensions were sonicated to disperse nanoparticle electrostatic aggregates and facilitate intravenous injection through thin-bore glass capillaries ...
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bioRxiv - Developmental Biology 2023Quote: ... using an S2 water-bath sonicator (Covaris), under the following parameters ...
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bioRxiv - Genomics 2023Quote: ... 400 ng of gDNA was brought up to 50 μL volume with 1X low-EDTA TE buffer and sheared with a Covaris E220 sonicator (Covaris, Inc., Woburn, MA) to an average 200 base pare size using the following settings ...
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bioRxiv - Immunology 2023Quote: ... purified ChIP DNA was fragmented using Covaris Focused-ultrasonicator S220 (Covaris) before library preparation ...
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bioRxiv - Genetics 2023Quote: ... 1μg genomic DNA was randomly fragmented by Covaris, and then the fragments were selected by Magnetic beads to an average size of 200-400bp ...
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bioRxiv - Microbiology 2023Quote: ... tissue was first cryo-pulverized using the CP02 CryoPrep automated dry pulverizer (Covaris). Trizol (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... We fragmented input gDNA by Covaris S220 Focused-ultrasonicator to an average insert size of 350 bp ...
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bioRxiv - Genomics 2023Quote: ... DNA was fragmented by a hydrodynamic shearing system (Covaris, Massachusetts, USA) to generate 180-280 bp fragments ...
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bioRxiv - Genomics 2023Quote: ... we transferred approximately 30 mg of liver tissue from each flash-frozen specimen stock to an extra thick TT05 TissueTube and cryo-pulverised the tissues into a rough powder using a cryoPREP (Covaris) automated dry pulverizer (2 impacts on intensity setting 6 with immersion in liquid N2 for 10 seconds between impacts) ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was fragmented with a Covaris S 220 instrument (Covaris). Libraries for RNA-Seq were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... (4) DNA was sheared to a size of 300-500bp using Covaris LE220 machine (Covaris) at the conditions ...
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bioRxiv - Genomics 2023Quote: ... 12 μl of DNA was first sheared using gTUBEs (Covaris) to target 15-18 kbp fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... or fragmented to a size of 15-25kb with a gTUBE (Covaris, 520079) centrifuged at 1,500rcf ...
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bioRxiv - Genetics 2023Quote: ... and fragmented using a Covaris LE220 ultrasonicator (Covaris, Woodburn, MA) targeting 400 bp ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of gDNA was fragmented by nebulization in g-tubes (Covaris, Woburn, MA, USA) to approximately 600 bp and subjected to end repair ...
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bioRxiv - Genomics 2023Quote: ... Then the sample was transferred into a miliTUBE with AFA fiber (Covaris Part# 520135) and topped up with lysis buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Half of the DNA was then sheared into 20 kb fragments with a COVARIS g-TUBE (Covaris, Inc., Woburn, MA, USA), centrifuging at 4,200 rpm for 1 minute to increase DNA throughput ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: ... Up to 2000 ng of double-stranded genomic DNA was fragmented by Covaris shearing to obtain fragment sizes of 160-180 bp ...
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bioRxiv - Genomics 2023Quote: 500 ng of ligated DNA was sheared into approximately 500 bp by Covaris M220 instrument in a 130 µl microtube ...
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bioRxiv - Genetics 2023Quote: ... The sheared DNA fragments (gTUBEs, Covaris, USA) underwent treatment with the SMRTbell Enzyme Cleanup Kit (Pacific Biosciences ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was sonicated to an average size of 500 bp using S2 Focused-ultrasonicator (Covaris) and end-repaired with NEBNext Ultra End Repair/dA Tailing Module ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 µg of genomic DNA was sheared to a target size of 300-400 bp with a Covaris E220 system (Covaris Inc., Woburn, MA, USA), cleaned on a small volume Mosquito liquid handler (TTP labtech ...
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bioRxiv - Genetics 2023Quote: ... DNA was sheared to a target size of ∼450-bp fragments using a sonicator (Covaris, Woburn, MA), using 4 x 14s cycles ...
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bioRxiv - Cell Biology 2023Quote: ... a product optimized for archived biosamples.29 The cDNA was sheared by Covaris, and the library was prepared and barcoded following the Ion Plus Fragment Library Kit protocol (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... Approximately 400 ng of DNA was fragmented by sonication using a Covaris S220 Focused-ultrasonicator (Covaris) and size-selected to enrich 400-600 bp fragments using SPRIselect beads (Beckman Courter) ...
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bioRxiv - Genetics 2023Quote: ... The sonication was performed with Covaris S220 Focused-ultrasonicator (Covaris). Immunoprecipitation was conducted with 1pg of a-FLAG antibody (F3165 ...
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bioRxiv - Genetics 2023Quote: ... This DNA was fragmented to 20 kb using a g-TUBE (Covaris) and molecules below approximately 10 kb in size were then depleted using the SRE XS kit (Pacific Biosciences of California) ...
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bioRxiv - Immunology 2023Quote: ... and fragmented by sonication (Covaris S220 ...
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bioRxiv - Genetics 2023Quote: ... DNA was sheared to a N50 of 10 kb using a g-tube (Covaris), 2 µg of DNA was sheared by centrifugation at 5500 rpm in an Eppendorf 5424r centrifuge two times for one minute each ...
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bioRxiv - Genetics 2023Quote: ... DNA was sheared to approximately 20 kb using g-tube (Covaris). Ultra- high molecular weight (UHMW ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 200 ng of DNA was fragmented by sonication using a Covaris S220 device (Covaris) and WGS DNA libraries were generated using a 0.7–0.8× ratio of AMPureXP beads for final size selection ...
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bioRxiv - Genomics 2023Quote: ... the genomic DNA was fragmented into fragments ranging from 400 to 550 base pairs (bp) through the utilization of the M220 focused-ultrasonicator (Covaris Ltd. Brighton, UK). The sequencing library was prepared with the TruSeq DNA Library LT kit (Illumina ...
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bioRxiv - Genomics 2023Quote: Purified gDNA was sheared to an average size of 1500 bp using ultrasonication (Covaris M220). Library input was examined on an Agilent bioanalyzer 2100 using the high sensitivity DNA Kit (cat ...
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bioRxiv - Genomics 2023Quote: ... 2-3x107 cells were collected and immediately processed for fixation using reagents provided in the truChIP Chromatin Shearing Kit with Formaldehyde (Covaris). After one wash with PBS ...
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bioRxiv - Genomics 2023Quote: ProteinA magnetic Dynabeads (40 μl per sample) were blocked in Chromatin IP buffer (Shearing Buffer D3 diluted with 2X Covaris truChIP IP Buffer and supplemented with 1X Protease Inhibitor Cocktail ...
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bioRxiv - Genomics 2023Quote: ... We quantified the purified DNA with a Qubit instrument (Thermo Fischer Scientific) to shear 330 ng of DNA into fragments of ∼ 300 bp with a Covaris sonicator (Covaris Inc.) and ...