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Citations for Mirus Bio :
801 - 850 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... co-transfection in 293GP cells with TransIT-LT1 Transfection Reagent (Mirus, 2300). Two days later ...
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bioRxiv - Immunology 2022Quote: ... The plasmid cocktail was then gently mixed with 15 μL of TransIT LT1 transfection reagent (Mirus, GA, USA). The growth media was replaced with Opti-MEM during transfection ...
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bioRxiv - Genetics 2022Quote: ... These vectors were used to produce retrovirus by transfection of HEK293T (ATCC) using Trans-IT (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: siRNA was delivered to differentiated adipocytes (6-7 days post-differentiation) using the TransIT-X2® dynamic delivery system (MIR 6004; Mirus Bio). Opti-MEM I (31985062 ...
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bioRxiv - Cancer Biology 2022Quote: ... The transient plasmid DNA transfection was performed using TransIT®-LT1 Transfection Reagent (Mirus, Madisom, WI). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Aag2 and U4.4 cells were transfected with 100 ng dsRNA and 250 ng RepDV2Rluc RNA per well using the TransIT-mRNA transfection kit (Mirus). As a positive control ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed using 16 μL of TransIT-LT1 (Mirus Bio) and 1 μg each of the eight plasmids encoding the corresponding influenza virus segments (a total amount of 8 μg of DNA) ...
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bioRxiv - Molecular Biology 2022Quote: HDRT and xHDRT DNA were Cy5-labeled using the Label IT® Nucleic Acid Labeling Reagents (Mirus) and used in a standard nucleofection protocol (see Cas9 ...
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bioRxiv - Molecular Biology 2022Quote: ... TamS cells were transduced using a lentivirus (Mirus Trans-Lenti Transfection Reagent ...
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bioRxiv - Biophysics 2022Quote: ... Transfection was achieved either via electroporation with a Lonza 4D Nucleofector unit or through chemical means using Mirus TransIT-Transfection Reagent (Mirus) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: HEK293T cells were transfected using TransIT®-LT1 (Mirus, Madison, WI) reagent with indicated expression vectors ...
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bioRxiv - Microbiology 2022Quote: ... 3μL of TransIT-2020 (Mirus Bio, Madison, WI) was added and the reactions were incubated for 30 minutes prior to addition to the cells in the BSL-3 ...
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bioRxiv - Neuroscience 2022Quote: ... was transfected into BHK cells with TransIT-mRNA Transfection Kit (Mirus Bio, Cat. No. MIR 2225). Virus containing supernatant was concentrated ...
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bioRxiv - Molecular Biology 2022Quote: Transfections were performed using Trans-IT LT1 (TLT-1) (Mirus Bio) according to the manufacturer’s instruction.
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bioRxiv - Biophysics 2022Quote: CHO-GFP-CCR5 cell lines were generated by transfecting CHO-K1 with a pCDNA3.1 Hygro-GFP-CCR5 construct using the TransIT-X2 transfection reagent from Mirus (MIR 6003). CCR5 expression was maintained by culturing cells in the presence of 400 µg/ml hygromycin and GFP-CCR5 expressing cells were isolated for single-cell cloning through the process of serial dilution cloning ...
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bioRxiv - Biophysics 2022Quote: ... and 3 μL of TransIt LT1 (Mirus #MIR2305). Stable clones were selected by growing in DMEM containing 0.5 μg/μL puromycin (Sigma-Aldrich #P8833-25MG ...
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bioRxiv - Bioengineering 2022Quote: ... DNA plasmid was labelled with Label IT® (Mirus, USA). Reagents used for immunofluorescence are listed in Supp ...
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bioRxiv - Biophysics 2022Quote: ... VSV-G and pAdVAntage packaging plasmids into 293FT cells using TransIT-LTI (Mirus). At 48 h post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... into HEK293T cells using TransIT-Lenti transfection reagent (Mirus Bio) and allowed to grow for 72 hours ...
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bioRxiv - Biophysics 2022Quote: ... HEK293T cells were transiently transfected by this construct using Mirus TransIT-293 (Mirus Corporation). Transfected cells were seeded for imaging or electrophysiology experiments 24 hours post-transfection.
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bioRxiv - Cell Biology 2022Quote: ... using TransIT-X2 transfection reagent (Mirus, MIR 6004). siRNAs against the different zDHHCs were previously validated in HeLa cells47 ...
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bioRxiv - Immunology 2022Quote: ... were transfected with pHR SIN including cloned transgene and packaging vectors pMD2.G and pCMVdR8.91 using TransIT-Lenti Transfection Reagent (Mirus #MIR6603). On the day of transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... and pREV using the TransIT-293 transfection reagent (Mirus Bio). About 18 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: Transient plasmid transfections were generally carried out using TransIT-LT1 (Mirus) transfection reagent or Polyethylenemine (Polysciences Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... Szczesny) and 0.8 μg EGFP-CHIP plasmid using Mirus reagents: 2 ul TransIT-293 (MIR 2700, Mirus) for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900 ...
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bioRxiv - Cell Biology 2022Quote: ... Transient transfections were performed with TransIt 293 reagent from Mirus. All cell lines were routinely checked for mycoplasma contamination and tested negative.
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bioRxiv - Microbiology 2022Quote: ... monolayers of BHK-T7 cells (4×105) cultured in 12-well plates were co-transfected using 2.5 μl of TransIT-LT1 transfection reagent (Mirus) per microgram of DNA plasmid ...
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bioRxiv - Microbiology 2022Quote: ... or HepG2C3A cells via the TransIT-mRNA transfection reagent (Mirus Bio LLC, Madison, WI) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... TransIT-X2 Dynamic Delivery System (Mirus Bio; MIR6004), following manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... We used a reverse transfection method (Mirus-IT LT transfection reagent, Mirus Bio) to introduce sgRNA containing plasmid into the CRISPRn/WTC-11 cells ...
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bioRxiv - Genetics 2022Quote: ... We used a reverse transfection method (Mirus-IT LT transfection reagent, Mirus Bio) to introduce sgRNA containing plasmid into the CRISPRn/WTC-11 cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 1.5 µg of the pHR plasmid along with two plasmids containing the lentiviral packaging proteins (0.167 µg of pMD2.G and 1.3 µg of p8.91) with TransIT-293 (Mirus Bio). After 2–3 d of transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Iso-2 and Cdk1 and its mutant plasmids were transfected into the cells using TransIT-X2 transfection reagent (Mirus, cat# MIR6000). siRNA oligonucleotides specific for Rad 51 were purchased from Sigma (ESIRNA HUMAN RAD51 EHU045521) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected using TransIT-X2 transfection reagent (Mirus, cat# MIR6000). In order to control for transfection efficiency cells were co-transfected with a plasmid expressing mCherry (pmCherry-C1)) ...
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bioRxiv - Cell Biology 2022Quote: ... and TransIT-293 (Mirus) regents ...
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bioRxiv - Cell Biology 2022Quote: MCF-10A cells were transfected with esiRNA at approximately 70–75% confluency usingTransIT-X2 (Mirus Bio) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10-cm dishes and allowed to reach ~70% confluency before transient transfection using TransIT-2020 (Mirus, catalog #: 5400), or siRNA treatment (50 nM ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were grown in 10-cm dishes and allowed to reach ~70% confluency before transient transfection using TransIT-2020 (Mirus), according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.2 µg of pVSVG) and 1.5 µg of lentiviral vector using TransIT-LT1 Transfection Reagent (Mirus). Lentiviral supernatant was harvested and filtered through a 0.45 µm PES filter.
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bioRxiv - Cell Biology 2022Quote: ... was accomplished using TransIT-LT1(Mirus, MIR 2300) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... as the DNA diluent and either TransIT-LT1 (Mirus, cat. MIR 2300), TransfeX (ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... 1ug of spike plasmid for each well was transfected into cells using transfection reagent (Mirus, TransIT-LT1) with adding DMSO or antiviral compounds for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Transfections of cells were performed with either Lipofectamine 2000 or TransIT-2020 / X2 (Mirus) reagents as recommended by the manufacturers.
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bioRxiv - Neuroscience 2022Quote: ... the TransIT-LT1 reagent (Mirus Bio #MIR2304) was used to deliver the SB 100x transposase and transposon vectors ...
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bioRxiv - Immunology 2022Quote: ... Cells were transiently transfected with 500 ng of total DNA and 1.5 µl of Transit X2 (Mirus Bio, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... pCDH-hIL8-EGFP plasmid was transfected into 293T cells using TransIT-Lenti (Mirus Bio). Viral supernatants were collected at 48 hours after transfection and transduced in THP-1 cells.
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bioRxiv - Cell Biology 2022Quote: ... were co-transfected in 293T cells using TransIT-Lenti (Mirus Bio). Viral supernatants were collected at 48 hrs and 72 hrs after transfection and centrifuged at 1,000 g for 10 mins ...
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bioRxiv - Genetics 2022Quote: ... a pooled sgRNA library with LentiCRISPR v1 vector was used to produce lentivirus by transfection of HEK293T (ATCC) using Trans-IT (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... plasmids encoding all ten reovirus genome segments were transfected into BHK-T7 cells using TransIT-LT1 (Mirus Bio). After the first signs of cytopathic effects ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected according to the manufacturer’s instructions using TransIT-LT1 (Mirus) at the ratio of 3 µL TransIT- LT1 to 1 µg DNA ...