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Citations for Mirus Bio :
951 - 1000 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Mirus Transit insect Transfection Reagent (Mirus bio) was used for the transfections according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... or 2 µg of pPol1II-HA and 2 µg of pPol1II-NA) using TransIT-293 (Mirus). Virus supernatant was harvested 72 hours post-transfection and filtered through 0.22 µm filters ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were transfected by electroporation using the Ingenio solution (Mirus) and a nucleofector (Amaxa) ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.2 μg pVSVG per 1 reaction in a 6-well plate well) into HEK 293T cells with TransIT-LT1 transfection reagent (Mirus #2304) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Monoclonal and polyclonal hIPSC-dCas9 lines were transfected with pCS2-iREP-GFP-PGK-BFP-U6-gRNA-irep or pCS2-iREP-GFP-PGK-BFP-U6-gRNA-mock vectors as replicates in 12 well plates using TransIT®-LT1 Transfection Reagent (Mirus Bio, MIR2300) according to manufacturer’s instructions and as detailed above ...
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bioRxiv - Cell Biology 2022Quote: hiPSC were transfected with pB-CAGGS-dCas9-KRAB-MeCP2 or pPB-dCas9-KRAB vectors together with mPBase transposase vector using TransIT®-LT1 Transfection Reagent (Mirus Bio,MIR2300) as recommended by the producer ...
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bioRxiv - Biochemistry 2022Quote: ... in 12-well plates and were transfected with 3 μg capped in vitro transcribed DENV replicon RNA using the TransIT-mRNA Transfection Kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 μg of lentiviral plasmid were added into a microcentrifuge tube with 7.5 μL of Mirus TransIT-Lenti transfection reagent (Mirus Bio, 6604). Once all plasmids were pooled together with the transfection reagent ...
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bioRxiv - Cancer Biology 2022Quote: ... or non-Targeting shRNA vectors pLKO.1 using Mirus Trans-IT TKO Transfection reagent (Mirus). 621-101 cells were transduced with lentiviruses for 48 hours and then selected against puromycin ...
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bioRxiv - Cancer Biology 2022Quote: ... Caco-2 cells were plated (10·106 cells) in 500μl of the Ingenio electroporation solution (Mirus Vio, Wisconsin, USA). The transfected cells were selected using the antibiotic geneticin/G418 (Gibco-Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... BHK-21 cells cultured in 12-well plates were transfected with 3 μg capped in vitro transcribed DENV2 RNAs using the TransIT-mRNA transfection kit (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... were labeled with Cy3 using a Label IT kit (Mirus). B16F10 cells were transfected with labeled sgRNAs and Cas9 protein (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 38HK were transiently transfected with lentiviral shRNA plasmids using TransIT-Keratinocytes Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: Cells cultured in a 24- or 96-well plate were transiently transfected with STF luciferase reporter and the internal control pRL-SV40 Renilla luciferase expression plasmid using TransIT-2020 (Mirus Bio), with or without Wnt1- or Wnt3a-expression construct as indicated in Results ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown to 70% confluency and siRNAs were transfected with Mirus TransIT-X2 (MIR6004, Mirus Bio) following manufacturer instructions in OPTI-MEM medium (31985070 ...
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bioRxiv - Cell Biology 2022Quote: ... and 1.2 μg cytomegalovirus 8.91 vector were prepared for transfection using TransIT-293 Transfection Reagent (Mirus Bio; Madison, WI). Three days post transduction virus-containing supernatants were harvested and concentrated approximately 40-fold using Lenti-X Concentrator (Clontech ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in 6-well plates or 10 cm dishes and allowed to reach ∼70% confluency before transient transfection using TransIT-2020 (Mirus Bio, Madison, WI, catalog #: MIR 5400) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... 10g of plasmid DNA was transfected into 293T lentiviral packaging cells using LT1 transfection reagent (Mirus Bio LLC), and viral supernatants were collected ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral vector was produced by transforming the produced LARRY plasmid into HEK293T cells using the Trans-IT 293 transfection reagent (Mirus #MIR2700). For each of eighteen 10 cm dishes of HEK293T cells ...
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bioRxiv - Cell Biology 2022Quote: ... A7r5 cells were transfected with two different combinations of mixed siRNA oligonucleotides against CLASP1 and CLASP2 using TransIT-X2 (Mirus). After 72 h ...
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bioRxiv - Cell Biology 2022Quote: ... carrying the sequence of the gene to be over expressed and resistance to puromycin into 0.8×106 HEK293 cells in a 60 mm culture dish with 6 µl of TrasnIT (Cat. Nº: Mirus Bio. 293) containing 3 ml of complete media ...
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bioRxiv - Cell Biology 2022Quote: ... and the desired transfer plasmid using TransIT-293 transfection reagent (Mirus). 48 hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Vectors were transfected into the cells using TranIT-X2 reagent (Mirus) according to the manufacturers protocol and grown on appropriate antibiotics to generate stable cell lines.
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bioRxiv - Cell Biology 2022Quote: ... Plasmid DNA was transfected using TransIT (Mirus Bio) by adding 1 µl of the reagent and 1 µg of DNA per 48-plate well ...
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bioRxiv - Cancer Biology 2022Quote: ... The ctDNA was fluorescently labeled with either Cx-rhodamine or CY5 using the Label IT® Nucleic Acid Labeling kit (Mirus Bio LLC, WI).
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bioRxiv - Cell Biology 2022Quote: ... Transfection were performed with the TransIT-X2 Transfection Reagent (Cat. MIR-6000, Mirus Bio). For CRISPR/Cas9-based knockdown of v-ATPase subunits ...
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bioRxiv - Cancer Biology 2022Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was made by transfecting Lx293T with standard packaging vectors and TransIT-LT1 Transfection Reagent (Mirus, MIR 2306). Viral supernatant was harvested two days after transfection ...
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bioRxiv - Genomics 2022Quote: ... Three px330-mCherry-sgRNAs vectors were co-transfected into H3.1 and H3.3 sensor cell lines using TransIT-X2 Transfection Reagent (Mirus Bio, MIR6003) as specified by provider’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Donor plasmids containing genes of interests were co-transfected with φC31-bacteriophage derived integrase in H11 V6.5 mESCs using TransIT-X2 Transfection Reagent (Mirus Bio, MIR6003) according to the provider’s instructions ...
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bioRxiv - Immunology 2022Quote: Using the TransIT-mRNA Transfection Kit (Mirus, Madison, WI), 1 × 107 293T cells were transiently transfected with mRNA (20 μg) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then plated at 0.8 million cells/well in a 6 well plate and transfected with either pTRIP-SFFV-Hygro-2A-mScarlet-HGS or pTRIP-SFFV-Hygro-2A-mScarlet-VPS37A with TransIT-293 (Mirus) (3µg DNA/well) ...
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bioRxiv - Immunology 2022Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of viral genomic DNA with TransIT-293 (Mirus) and left O/N ...
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bioRxiv - Immunology 2022Quote: ... CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio, WI, USA), according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 8 µL of Trans-IT LT1 (Mirus, #2300) was added to Opti-MEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... The next day cells were transfected using TransIT 293 transfection reagent (Mirus), following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and mRNA-1285 at various concentrations using a TransIT mRNA transfection kit (Mirus Bio, Madison, WI). At 24 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... using Trans IT (Mirus Bio) according to the manufacturer protocol.
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bioRxiv - Evolutionary Biology 2022Quote: ... TransIT-LT1 transfection reagent (Mirus Bio LLC ...
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bioRxiv - Plant Biology 2022Quote: ... using the cationic lipid polymer TransIT-2020 (Mirus, Madison, WI) as previously described (Svitashev et al ...
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bioRxiv - Synthetic Biology 2022Quote: ... TransIT®-2020 transfection reagent (Mirus #MIR5400) was used to transfect PiggyBac Transposon system plasmids into parental HEK293T/17 cells to engineer stable antigen-presenting cells (APCs ...
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bioRxiv - Synthetic Biology 2022Quote: ... and transfected with a total of 2.5 μg of SSG-encoding DNA/well using TransIT-2020 Transfection Reagent (Mirus Bio, #MIR5400) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... with pRL-EMCV-FL reporter plasmid and the indicated pCAGGS-based nsp1 expression plasmids using TransIT-LT1 reagent (Mirus). At 24 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... a T25 flask of BHK-21 cells at 75% confluency was transfected with 10 μg of MAYV CMV-promoter driven plasmid using TransIT-LT1 Transfection Reagent (Mirus) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: HeLa cells were seeded in 6 well plates (2.0×105 per well) and incubated overnight before transfection with pCAGGS-VP40 using lipofectamin LT-1 (MIR2304; Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... by Trans IT LT-1 (Mirus Bio, Madison, WI). Single cell clones were established by the limit dilution method ...
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bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was produced by transfecting HEK293T cells with standard packaging vectors using the TransIT-LT1 Transfection Reagent (MIR 2306; Mirus Bio LLC). Viral supernatant was collected 48–72 h after transfection ...
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bioRxiv - Zoology 2022Quote: ... Four µg of either plasmid were transfected into 6 × 105 C6/36 cells using TransIT-2020 transfection reagent (Mirus) for 4 h ...
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bioRxiv - Microbiology 2022Quote: ... Transfection of EILV-eGFP RNA was performed using the TranIT-mRNA Transfection Kit (Mirus Bio, Madison, WI, USA). We verified EILV-eGFP infection in transfected cells by examining them for eGFP fluorescence using a Zeiss Axiovert S 100 equipped with a Fourier-transform infrared spectroscopy (FTIR ...
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bioRxiv - Microbiology 2022Quote: Recombinant HIV-1 variants were prepared by transfection of the pNL4.3 mutants into HEK293T cells using TransIT-293 (Mirus, Houston, TX, USA) and Opti-MEM I medium (Thermo Fisher ...