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Citations for Mirus Bio :
701 - 750 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... XGal staining was performed using the beta-galactosidase staining kit (Mirus #MIR 2600), following the provided protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293Ts were transfected with TransIT-Lenti (Mirus Bio Cat. #Mir6603) reagent ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected using TransIT-293 transfection reagent (Mirus Bio LLC). For transient expression of human Siglec-5-GFP 80% confluent HEK293 in 8 well chamber slide (0.25 ml/well ...
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bioRxiv - Molecular Biology 2023Quote: ... and rev genes and pMD2.G encoding the VSV-G envelope protein using the TransIT-Lenti transfection reagent (Mirus, MIR 6650). The virus was harvested after 48 hours and filtered through a 0.45 μm filter ...
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bioRxiv - Biophysics 2023Quote: ... We generated VLPs as follows: 57 μL of Trans-IT reagent (Mirus) were added to 2 μg of pR8ΔEnv.2 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were reverse transfected with expression plasmids via TransIT-X2 (Mirus) at a 1:3 ratio (2.5 μg plasmid ...
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bioRxiv - Neuroscience 2023Quote: ... 90 μL of TransIT-LT1 (Mirus Bio, Cat. No. MIR6600) was diluted into 3 mL of Opti-MEM and incubated at RT for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... pCMV R8.74 and pCDH1-CMV-ATG3-mCherry-SV40-Hygromycin using TransIT-LT1 reagent (Mirus). After 72 hr post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and pHR: mU6-sgRNA-EF1A-puro-P2A-BFP (sgRNA-BFP) (1:1:1) using 24 μL TransIT®-LT1 Transfection Reagent (Mirus Bio, Cat. No. MIR 2300) in a final volume of 1000uL Opti-MEM medium (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of PCR product was fluorescently labeled using the Label IT-Cy5 Nucleic Acid Labeling Kit (Mirus) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... or TransIT-X2 (Mirus) in a 6-well plate (10 μl Lipofectamine + up to 100 μl of BAC-VZV DNA) ...
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bioRxiv - Biophysics 2023Quote: ... Transfections were performed 20 hours following seeding of 2x104 HEK 293T cells per well in 96-well plates and contained 70 ng of ABE8e plasmid and 30 ng of sgRNA expression plasmid mixed with 0.3 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... and 15 ng each of two sgRNA expression plasmids mixed with 0.3 µL of TransIT-X2 (Mirus) in a total volume of 15 µL Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293T cells were transfected at 60-90% confluence with TransIT®-LT1 Transfection Reagent (#2300, Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Lentiviruses for transduction of LTR-mCherry reporter and expression of HIV receptors (CD4, CXCR4, and CCR5) was generated by transfection of HEK293 cells using the Mirus TransIT transfection kit (Mirus). The resulting supernatant was cleared of debris by low-speed centrifugation (300g for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with Tat mRNA using the Mirus TransIT-mRNA transfection kit (Mirus). Cells that produced mCherry signal after transfection were isolated from the array and isolated as monoclonal populations into 96-well plates for expansion.
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bioRxiv - Microbiology 2023Quote: 3 x 105 cells were seeded per well in 6-well plates and transfected one day after seeding with 3CLpro plasmids using TransIT-PRO (Mirus Bio LLC) and incubated 8-9 hours for Mpro-On and 10 hours for Mpro-Off assays ...
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bioRxiv - Immunology 2023Quote: ... or transfected using TransIT-LT1 (Mirus Bio, USA) with HT-DNA (5 µg/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... were done by mixing 2.5 µg of plasmid DNA with 7.5 µl of TransIT-X2 reagent (Mirus, Madison, WI). The mixture was added to 105 CAF or PDAC cells cultured in serum-free and antibiotic-free media overnight.
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bioRxiv - Cancer Biology 2023Quote: All lentiviruses were produced by co-transfection of HEK 293T/17 cells with plasmid DNA for the respective lentiviral vector and the packaging components psPAX2 and pMD2.G at a 5:3:2 mass ratio (vector:psPAX2:pMD2.G) using TransIT-LT1 (Mirus MIR 2300). Lentiviral supernatants were collected at 48 and 72 hours post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Transient transfections were performed using TransIT X2 (Mirus, MIR6003) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 16 hpt using TransIT X2 (Mirus) transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with 1.5 μl X2 Transfection Reagent (Mirus) and 200ng plasmid DNA (pEGFP-C2 containing an insert derived from CIZ1) ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were transiently overexpressed using TransIT-LT1 (Mirus, MIR2306) according to the instructions of the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... Transfection was performed using TransIT-LT1 (Mirus) transfection reagent according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... TransIT-X2® transfection reagent was purchased from Mirus (Cat #6000) and used as received.
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bioRxiv - Evolutionary Biology 2022Quote: ... LabelX solution was prepared by reacting Label-IT (Mirus Bio) with Acryloyl X – SE (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Transient transfections were performed with TransIT-293 (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... or TransIT-LT1 (Mirus Bio). RNA PAMP transfections were done using the TransIT-mRNA Transfection kit (Mirus Bio) ...
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bioRxiv - Immunology 2022Quote: ... RNA PAMP transfections were done using the TransIT-mRNA Transfection kit (Mirus Bio). The siRNA transfections were done using Lipofectamine RNAiMax (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using TransIT-LT1 reagent (Mirus; Madison, WI, USA), according to manufacturer recommendation ...
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bioRxiv - Physiology 2022Quote: ... cells were transfected with 25mM EP300 siRNA or scramble control siRNA via the TransIT-X2 Dynamic Delivery System (Mirus Bio), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Individual or pooled sgRNAs were introduced into CRISPRi- or CRISPRa-iPSCs via lentiviral delivery using TransIT Lenti Reagent (Mirus Bio LLC; Cat. No. MIR 6600) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Preadipocytes were transfected by seeding 30 000 cells per well of a 24 well plate in growth medium and adding 1.5 μl TransIT-X2 (Mirus) and 125 ng plasmid DNA or 1.4 μl LNA (10 μM ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was done using the TransIT-LT1 Transfection Reagent (Mirus). Two solutions were prepared for each well ...
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bioRxiv - Genomics 2022Quote: Transection was performed in a 10-cm dish at 70%-80% confluence with 30 ul of TransIT 2020 transfection reagent (Mirus Bio, MIR 5400A) and 10μg of total DNA (1µg of pMT-ROWx2FLAG and 9 µg of bluescript plasmids) ...
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bioRxiv - Cancer Biology 2022Quote: ... with Trans-IT 293T (Mirus). 293FT supernatants were harvested at 24 ...
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bioRxiv - Cell Biology 2022Quote: Viral particles were produced by transfection of 293FT cells in 6-well plates with 3 μg of total DNA and 8 mL TransIT-293 Transfection Reagent (Mirus Bio) per well ...
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bioRxiv - Cell Biology 2022Quote: ... SiRNA was transfected using TransIT®-LT1 Transfection Reagent (Mirus Bio, Madison, WI) as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μg PMD2.G packaging plasmids using 25 μl TransIT®-LT1 Transfection Reagent (Cat# MIR 2306, Mirus). After 72 h of transfection ...
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bioRxiv - Bioengineering 2022Quote: ... or TransIT TKO (Mirus) following manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: Transfection of human pancreatic MIA PaCa-2 and BxPC-3 cells was performed with TransIT-mRNA (Mirus Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and 1.5 μg of the plasmid containing the gene of interest were cotransfected by using TransIT transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the packaging plasmid psPAX2 and the envelope plasmid pVsVg using TransIT- Lenti transfection reagent (Mirus). The transfection mix was incubated for 20 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and with 21 μL of TransIT-Lenti (Mirus #6603). The mixture was incubated for ~15 min and then added dropwise to the 10 cm dish containing Lenti-X cells ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... The VSV-G pseudotyped lentivirus was packaged by 5 plasmid co-transfection of HEK293T cells using TansIT Transfection Reagent (Mirus Bio) in 15 cm culture dishes (The protocol describing production of lentiviral particles can be found at http://www.www.bu.edu/dbin/stemcells/protocols) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of TransIT-X2® transfection reagent (Mirus Bio) was then added to the tube ...
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bioRxiv - Cell Biology 2022Quote: ... was added to 100 μL Opti-MEM medium to a final concentration of 900 nM with 7.5 μL transfection reagent TransIT-X2 (Mirus Bio), mixed and incubated at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... constructs were packaged into lentivirus in a 10 cm plate 60% confluent of HEK293T cells using the TransIT-LT1 (Mirus Bio, MIR2300) transfection reagent and 7 μg of the vector ...
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bioRxiv - Cell Biology 2022Quote: ... for transfection of HEK293 Flp-In T-REx cells and 2 ul Trans-IT-HeLa and 1.3 ul Monster (MIR 2900, Mirus) for HeLa Flp-In T-REx cells ...