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Citations for Mirus Bio :
851 - 900 of 1576 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... using Mirus 2020 DNA transfection reagent (Mirus). The infectious virions were harvested in the culture supernatant 48 h post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... purified replicon RNA was transfected into HeLa cells grown on 96 well plates using TransIT mRNA transfection reagent (Mirus), and the cells were incubated in the growth medium supplemented with 5µM of cell-permeable Renilla luciferase substrate EnduRen (Promega ...
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bioRxiv - Genomics 2022Quote: ... in HK-2 human proximal tubule cells at approximately 70% confluency in 96-well plates by using TransIT-2020 Reagent (Mirus, #5404), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... and pHelper vectors using TransIT293 reagent (Mirus, 2700). After 72 hours ...
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bioRxiv - Cell Biology 2022Quote: ... transient transfection was performed by mixing transfection reagent (TransIT®-2020; Mirus Bio) with a reagent-to-DNA ratio of 3:1 ...
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bioRxiv - Immunology 2022Quote: Transit®-2020 and Transit®-Lenti (Mirus), Polybrene (Millipore) ...
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bioRxiv - Immunology 2022Quote: ... Lentiviral particles were generated in HEK293T cells transfected with the packaging vector pCL-Ampho (Imgenex) and the construct of interest using Transit®-Lenti (Mirus) in Opti-MEM (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... Primers were designed for the predicted coding region of Bma-LEC-1 and Bma-LEC-2 that incorporated restriction digest sites facilitating recombination into the pOETIC 6xHis Transfer Plasmid (Mirus Bio, Madison, WI) (Supplemental Materials 1) ...
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bioRxiv - Immunology 2022Quote: ... Positive recombinant plasmid was transfected into Sf21 cells using the Flashbac Ultra Baculovirus Expression System (Mirus Bio). Viral Titers were determined with the BacPak qpcr Titration kit (Takara Bio ...
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bioRxiv - Cell Biology 2022Quote: ... The following day 3 µl of Mirus TransIT 2020 reagent (Mirus Bio, Madison, WI) and 1 µg of plasmid were combined in 250 µl Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and NP proteins (23) were transfected into 50% confluent HEK293T cells in 6-well plates with TransIT-LT1 transfection reagent (Mirus Bio) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... plasmid was fluorescently labeled with the Cy5 fluorophores using the Mirus Label IT® tracker intracellular nucleic acid localization kit (MIR 7021, Mirus Bio, Madison, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in RPE1 or HeLa cells for 24–48 h using TransIT®-LT1 Transfection Reagent (Mirus Bio) or Neon Electroporation (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... by TRANS-IT (TRANS-IT X2, Dynamic delivery System, Mirus, USA) following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviral particles were made in Plat-A packaging cells using TranstIT-LT1 (Mirus). RMS cells were infected with viral particles for 24h at 37° C using 8 mg/mL of polybrene (EMD Millipore) ...
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bioRxiv - Cell Biology 2022Quote: ... using TransIT-Lenti transfection reagent (Mirus) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Interactions with codon-optimized MECR- and cMECR-mCh fusions were tested by transfecting 293T cells with mammalian expression constructs using TransIT-X2 (Mirus) for 48 h ...
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bioRxiv - Cell Biology 2022Quote: ... Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus), and Opti-MEM Reduced Serum Medium (Gibco) ...
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bioRxiv - Immunology 2022Quote: Monolayers of BSC-40 cells were infected with parental IHD-J at an MOI of 0.01 PFU/cell and transfected 1 hour later with 10 μg of DNA of plasmid pCyA-ISG15GG (pCyA was kindly provided by Mariano Esteban) using LT-1 (Mirus Bio) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... pcDNA3.1-N-MYC-BioID2-rat mTOR (rmTOR) using the TransIT-X2 transfection reagent (Mirus) according to the manufacturer’s specification ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected using TransIT-Insect Transfection reagent (Mirus, MIR6105). Cells were harvested 48 hrs post-transfection and samples were processed and analyzed following the procedure described above.
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bioRxiv - Microbiology 2022Quote: All transfections in this study were performed with TransIT-X2® Transfection Reagent (Mirus Bio, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RNAs encoding green fluorescent protein (GFP) were complexed with TransIT (Mirus Bio, Madison, WI) according to the manufacturer’s instructions by diluting in EMEM without serum or antibiotics and incubating for 2-5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... The vectors were transfected into A549 cells in 96-well microtiter plate using TransLT transfection reagent (Mirus) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Phoenix Ecotropic (pECO) cells (ATCC CRL-3214) were transfected with plasmids using TransIT-293 transfection reagent (MIR2705, Mirus). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Label IT Cx-Rhodamine and Cy5 were purchased from Mirus Bio ...
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bioRxiv - Cancer Biology 2022Quote: Cells were transfected with the indicated specific short interfering RNA (siRNA) SMARTpools (Dharmacon, Cat# L-004594-00-0005 and Cat# D-001810-10-20) using TransIT-X2 Transfection Reagent (Mirus) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and vector plasmids were cotransfected into HEK293T cells using TransIT®-293 (Mirus Bio, Madison, WI). Lentiviral supernatants were harvested at 48-72 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... using the TransIT-X2 Dynamic Delivery System (Mirus Bio) for 48 hours followed by the addition of 100u/ml IFN-γ for 16-24 hours prior to harvesting and seeding MDMs onto micropatterns ...
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bioRxiv - Immunology 2022Quote: Viral particles were produced by transfection of HEK293FT cells in 6-well plates with 3μg DNA and 8μL TransIT-293 (Mirus Bio) per well ...
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bioRxiv - Molecular Biology 2022Quote: ... and MirusIT 2020 (Mirus) were used to transfect HEK293T ...
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bioRxiv - Molecular Biology 2022Quote: ... 6×105 cells were resuspended in 100 μL Ingenio Electroporation Solution (Mirus), and RNP complex with either 100 pmol ssODN or 7.5 μg plasmid donor DNA was added to the cell suspension ...
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bioRxiv - Molecular Biology 2022Quote: ... TransIT-HelaMonster (Mirus) and MirusIT 2020 (Mirus ...
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bioRxiv - Microbiology 2022Quote: ... the unsealed CPER product was first transfected into HEK293T cells by using TransIT-LT1 (Mirus Bio), and the trypsinized cells were then overlaid onto Vero E6-TMPRSS2 cells at 6 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... The final CPER product was transfected into Vero E6-TMPRSS2 cells seeded into 6-well plates (~ 5 × 105 cells per well) by using the TransIT-X2 Dynamic Delivery System (Mirus Bio) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Two doses of unformulated mRNA (500 ng/ml and 100 ng/ml) were transfected in parallel using a TransIT-mRNA transfection kit (Mirus). For analysis of membrane-bound antigen expression ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected with 7.7 µg of a plasmid (split evenly by mass between the plasmids indicated) using TransIT-LT1 (Mirus) and after 18 h the cells were mock-treated (refreshed media ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1.5 µg of a pLKO.1-Hygro or lentiCas9-Blast plasmid using the TransIT-LT1 Transfection Reagent (Mirus Bio) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The genomic RNA was transfected into BHK-21 cells with TransIT-mRNA reagent (Mirus), and culture supernatants were collected when CPEs were observed.
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bioRxiv - Molecular Biology 2022Quote: ... Cells containing RNP and donor DNA were electroporated at 150V using the Ingenio EZporator Electroporation System (Mirus). Cells were incubated at ambient temperature for 5 min and transferred to a six-well plate containing 2 mL growth medium with or without combinations of 1 μM AZD7648 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2 μg of DNA 24 - 48 hours before measurement using TransIT transfection reagents (Mirus). DRG neurons from adult (postnatal weeks 8–12 ...
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bioRxiv - Microbiology 2022Quote: ... Transfections were performed using TransIT-LT1 transfection reagent (Mirus), following the protocol described for the generation of recombinant fluorescently marked RVFV-35/74 variants.
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bioRxiv - Microbiology 2022Quote: ... Transfections were performed using TransIT-LT1 transfection reagent (Mirus), with slight modifications to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-293 TREx FlpIn EMX1-EGFP reporter cells were cultured in DMEM supplemented with 10% (v/v) FBS and 50 μg/mL hygromycin (Mirus).
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bioRxiv - Molecular Biology 2022Quote: ... Cells with successful integration of the reporter locus were selected using hygromycin (Mirus Bio).
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bioRxiv - Plant Biology 2022Quote: ... the purified miRNAs were labeled with Cy5 fluorophore (Cy5 Labeling Kit; Mirus Bio LLC) and then subjected to hybridization with the customized microarray for 4 hours in a 42°C oven ...
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bioRxiv - Microbiology 2022Quote: 3×105 cells were seeded in 6-well plates and transfected with 3CLpro plasmids via TransIT-PRO (Mirus Bio LLC, Madison, WI, USA) and incubated overnight ...
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bioRxiv - Microbiology 2022Quote: 3×105cells were seeded in 6-well plates and transfected with 3CLpro plasmids via TransIT-PRO (Mirus Bio LLC, Madison, WI, USA) and incubated overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... pCMV6-AN-HA-LARP1-DM using the TransIT-X2 transfection reagent (Mirus) according to the manufacturer’s specification ...
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bioRxiv - Molecular Biology 2022Quote: ... using the TransIT-X2 transfection reagent (Mirus) according to the manufacturer’s specification ...