Labshake search
Citations for abcam :
1201 - 1242 of 1242 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
-
bioRxiv - Molecular Biology 2021Quote: ... H4K20me1 or H4K20me3 IP was performed overnight in TD buffer as described above using 5 µg of H4K20me1 or H4K20me3 antibody (Thermo Fisher Scientific # MA5-18067, Abcam # ab9053 respectively). After antibody capture on protein G magnetic beads and 3 washes with TD buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... then incubated in 5% milk in TBS-T containing 1:10000 horseradish peroxidase (HRP) conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: 5-FU and wdr12 samples were immunolabeled with anti-HuC/D antibodies (not shown) (ab210554, abcam, used at a dilution of 1:600). Anti-GFP (GFP-1020 ...
-
bioRxiv - Cancer Biology 2020Quote: ... the membranes were placed in 5% nonfat milk for blockage and probed with the primary antibodies against TEAD4 (ab151274, Abcam, Cambridge, MA, USA), PKM2 (ab137852 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were incubated overnight at 4C at the following dilutions in a 5% serum buffer in PBS: LCK (ABCAM Ab227975, 1:200), FYN (BD Biosciences BD61063 1:150) ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were pre-incubated at room temperature for 2 hours in a blocking medium composed of PB-Tx and Normal Goat Serum (NGS, 5% or 10%, Abcam, Cat. No. ab7481), after which they were incubated with primary antibodies (72 hours at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated either at room temperature for 2 hours or overnight at 4°C with primary antibodies in 5% milk in 1X TTBS at the following concentrations: DNAJB6 (Abcam #ab198995; 1:1000), CTCF (Cell Signaling Technology #2899 ...
-
bioRxiv - Neuroscience 2022Quote: ... Co-staining of astrocytes and endothelial cells were performed with antigen retrieval (boiling in sodium citrate buffer in pressure cooker for 5 min) and probing with GFAP-488 (eBioscience 53-9892-80, 1:400) and CD31 (Abcam 28364, 1:200).
-
bioRxiv - Cancer Biology 2024Quote: Immunofluorescence (IF) was performed on whole FFPE 5-µm-thick tissue sections using anti-TP63 mouse monoclonal antibody (1:100; Abcam, catalog no. ab735), anti-CK8 rabbit polyclonal antibody (1:100 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were incubated at 4°C for 72 hours in blocking solution (5% bovine serum albumin, 0.2% Triton-X) containing primary antibodies CD45 antibody (Abcam, ab10558, 1:400 dilution), CD11b (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked with 5% non-fat milk for 1 h and incubated with primary antibodies against β-actin (1:1000, Abcam, cat# ab8226), PI3K (1:1000 ...
-
bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
-
bioRxiv - Neuroscience 2024Quote: ... The following antibodies were purchased from the manufacturers described: mouse anti-tau antibody (tau-5; Cat# ab80579) and rabbit anti-GAPDH antibody (Cat# ab181602) from Abcam (Cambridge, United Kingdom), rabbit anti-tau polyclonal antibody (Cat# A0024 ...
-
bioRxiv - Cancer Biology 2024Quote: ... After blocking with 5% skim milk (Beyotime, China) and sequential incubation with the primary and secondary antibodies: anti-PRMT3 (Abcam, Ab191562, 1:2000), anti-PRMT3 (Proteintech ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were then blocked using 5% nonfat milk for 1 h and incubated with primary antibodies against VASN (cat# ab156868, abcam, 1:500 dilution), IL33 (cat# A8096 ...
-
bioRxiv - Molecular Biology 2020Quote: ... After blocking in 5% milk/TBST membranes were probed with primary anti-ACE2 antibody (Abcam 15348 for total ACE2 or Abcam 108252. for long ACE2) followed by the appropriate secondary HRP-conjugated antibody (Dako) ...
-
bioRxiv - Genomics 2022Quote: ... A cocktail of antibodies against the glucocorticoid receptor was added to chromatin input for immunoprecipitation: 2 μg of ab3570 (Abcam, lot GR3222141-5, discontinued) and 0.49 μg of 12041S (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in 5% milk TBS-T and incubated with blots at 4°C overnight (BMAL1, Abcam – ab93806; ACTIN, Abcam – ab3280, P84, Genetex – GTX70220). Blots were incubated with HRP-conjugated secondary antibodies (Mouse IgG-HRP conjugate ...
-
bioRxiv - Genetics 2020Quote: ... chromatins from freshly isolated or cultured SCs were fragmented using sonicator and incubated with 5 μg antibody against histone H3-K27 acetylation (Abcam, ab4729, rabbit poly-clonal) overnight at 4 °C ...
-
bioRxiv - Immunology 2020Quote: ... Slides were incubated in Antibody Diluent (Zytomed Systems, Berlin, Germany) in the presence of a primary antibody specific for WNT6 (purchased from Abcam (ab50030, 5 μg/ml) or Bio-Techne (AF4109 ...
-
bioRxiv - Immunology 2020Quote: ... fluorescent antibody— were added in 1/50 and 1/100 dilutions of the antibodies combined in 5% BSA in PBS and/or rabbit isotype control (Abcam Ab172730 diluted 1/50), and incubated overnight at 4° C in a dark moisture chamber ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam, #ab119684, 1:1000 dilution) at 4 °C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... in 1X TBS-T with 5% (w/v) BSA and then with anti-rabbit HRP-conjugated secondary antibody (Abcam, Cat # ab205718; diluted 1:7,500). For signal visualization ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.2% Tween 20) with 5% milk and incubated overnight at 4 °C with the primary antibodies: rabbit monoclonal anti-histone H2B (Abcam Cat#ab188291, 1:2000) or mouse monoclonal anti-beta actin (Abcam ...
-
bioRxiv - Microbiology 2024Quote: ... Membranes were incubated in a blocking solution (0.1% PBST + 5% Milk) for 30 minutes at room temperature and then probed with primary antibodies (rabbit anti-β-actin (Abcam, AB8227, 1:1,000) and guinea-pig anti-GLT-1 (Millipore Sigma ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were then blocked for 1 hour in 5% goat serum and PBS and then incubated overnight at 4° C with CD45-specific (Abcam, Cat# ab154885, 1:100) or AhR-specific (Abcam Cat# ab213480 ...
-
bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibody incubation was performed overnight at 4°C using the following antibodies: anti-GFP rabbit (Abcam, diluted 1:2.000 in 5% milk in TBST) and anti-actin (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... table 1) were diluted in blocking solution (F4/80 [Bio-Rad]: 10 µg/ml, Ly6G [abcam]: 1 µg/ml, MHC-II [BioLegend]: 5 µg/ml), CD163 [Invitrogen] ...
-
bioRxiv - Developmental Biology 2021Quote: ... Membranes were blocked in 5% BSA-TBST (TBS with 0.1% Tween20) and probed with anti-Padi4 (Abcam ab214810, 1:1000, O/N at 4°C) and anti-Actin (Santa Cruz ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with a buffer at 4°C overnight prior to being incubated with primary antibodies anti-5-HT1A (Abcam #ab227165, rabbit pAb, 1:1000), anti-5-HT1B (Abcam #ab13896 ...
-
bioRxiv - Genomics 2023Quote: ... were blocked and pH-calibrated as per the manufacturer’s protocol and 5 μl bed volume of beads were loaded with 2 μg of ChIP antibody (H3K27ac; Abcam-AB4729, H3K115ac; PTM BIO-PTM-170). Beads were added to the solubilized chromatin ...
-
bioRxiv - Biochemistry 2022Quote: ... and re-probed (1h, RT, 1/10000 diluted in PBS-Tween with 5% non-fat milk) with rat anti-β actin (AbCam, Cambridge, UK, RRID: AB_867488) washed (as above ...
-
bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 5% non-fat milk in TBS-T and incubated with primary antibody rabbit Anti-MAP2 (1:1000 dilution, Abcam, Cambridge, MA, catalog no. ab183830). Anti Rabbit-HRP (Invitrogen G-21234 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Brains were incubated in primary antibody (rat anti D-Cad2, DSHB, 1:20, rabbit anti cleaved Dcp-1, CST cat#9578, 1:100, or rat anti Miranda, Abcam CD#5-7E9BG5AF4, 1:500) diluted in 1x PBS 0.3% Triton X-100 overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... tumor cells were stained using antibodies against chicken anti-GFP (Novus, cat #NB100-1614, concentration 10 μg/mL) and rabbit anti-NR2F1 (Abcam, cat #ab181137, concentration 5 μg/mL), goat anti-SOX9 (RD ...
-
bioRxiv - Cancer Biology 2021Quote: ... tumor cells were incubated overnight at 4 C in the presence of primary antibodies against chicken anti-GFP (Novus, cat #NB100- 1614, concentration 100 μg/mL) and rabbit anti-NR2F1 (Abcam, cat #ab181137, concentration 5 μg/mL), or rabbit anti-SOX9 (Millipore ...
-
bioRxiv - Cancer Biology 2021Quote: ... Slides were then incubated overnight at 4 C with primary antibodies against chicken anti-GFP (Novus, cat #NB100-1614, concentration 100 μg/mL) and rabbit anti-NR2F1 (Abcam, cat #ab181137, concentration 5 μg/mL), rabbit anti-SOX9 (Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... The global 5mC of the 30 snails per treatment was measured by the dot blot method based on the recognition of 5 methylcytosine (5mC) by anti-5mC antibody (Abcam, Cat. No. ab73938, Lot: GR278832-3) as described previously (Luviano et al. ...
-
bioRxiv - Bioengineering 2024Quote: ... 0.5% Triton-X in PBS) for 30 min and an additional wash with Goat F(ab) anti-mouse IgG (Abcam; ab6668, 2 μg/mL in blocking buffer) was performed for 1 h ...