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Citations for abcam :
1151 - 1200 of 1242 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... incubated with blocking buffer (PBS + 5%FCS +0,1%Tween) for 1 hour and stained with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and /or anti-TOM20 antibody (diluted 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... using a rabbit anti-β-tubulin at 1:500 dilution in 5% milk + TBS-T and 0.02% sodium azide (abcam, ab15568) combined with an anti-rabbit-HRP in a dilution of 1:4000 (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked with blocking buffer (5% goat/mouse serum in 0.3% triton-x100 in 1xPBS) and stained with rabbit anti-human CD3ζ (abcam cat# Ab226263). Coverslips were then gently washed 3 time with 1x PBS for 10 minutes each and stained with Donkey anti- Mouse IgG Antibody ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed 3 x 5 min and stained for 10 min with 1:50 DAB Chromogen in DAB substrate (Abcam ab64238). Slides were rinsed 4x ...
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bioRxiv - Neuroscience 2024Quote: ... brains and proboscis were washed 5 times for 10 minutes with PB + 0.3% Triton X- 100 (PBT) and blocked for 1 hour in PBT + 5% normal goad serum (NGS) (Abcam, ref. ab7481). Later ...
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bioRxiv - Neuroscience 2024Quote: ... 800 µl of chromatin was mixed with 3200 µl of chromatin dilution buffer and 5 µg of rabbit polyclonal anti-V5 (Abcam, ab15828) or normal rabbit IgG (Merk ...
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bioRxiv - Cancer Biology 2024Quote: ... for nuclear counterstaining for 5 min at room temperature and the slides were mounted with Anti-Fade Fluorescence Mounting Medium (Abcam, ab104135) before examination under an AxioScan7 microscope (Zeiss ...
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bioRxiv - Cell Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in TBST containing 5% BSA: DYRK1A (1:1000; Abcam, ab65220), FAM53C (1:1000 ...
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bioRxiv - Genetics 2024Quote: ... The next day the blot was washed 3 times for 5 minutes in PBST before adding 1:500 dilution of anti-Ret antibody (Abcam: ab134100) in PBST and with overnight shaking at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The mouse anti-DP Yun (#6, 1:5 (Du et al., 1996) and the mouse beta actin (1:1000, Abcam, Cat#ab8224) antibody was used as loading control in western blot assays.
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bioRxiv - Microbiology 2021Quote: ... Fixed worms were washed three times in PBS for 5 mins and transferred to new 12-well plates with 1ml per well of 1x Phalloidin-iFluor 647 Reagent (Abcam cat# ab176759) in PBS pH=7.4 for 90 mins at room temperature with moderate shaking ...
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bioRxiv - Neuroscience 2020Quote: ... The fixed cells were blocked with 5% normal goat serum for 4 hours followed by incubation with anti-Iba-1 antibody (1:300, Abcam, Cambridge, UK) for 12 hours at 4°C to label microglial cells ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Biochemistry 2020Quote: ... After blocking with 5% defatted milk, membranes were incubated (overnight, 4°C) with primary antibodies (anti-NNT cat. Abcam: ab110352; β-actin cat. Abcam ab8226). Membranes were then incubated with secondary antibodies conjugated to fluorescent (Licor® ...
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bioRxiv - Pathology 2021Quote: ... The samples were then washed for 5 minutes three times with TBT and incubated with the secondary antibody (Thermofisher A-21121 and Abcam® ab97239) for an hour at a dilution of 1:200 ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was adjusted to 400 µg and incubated O/N at 4℃ with 5 µg of IP-antibody (Table S1) or rabbit IgG control (Abcam, Cambridge, UK) in a final volume of 500 µl of NT2 buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: Sections were briefly dried then washed 3x in 0.1% Triton X-100 in phosphate buffered saline (PBST) before they were blocked with 5% neutral goat serum (Abcam or Vector Labs). Sections were then incubated for double-labeling experiments with primary antibody (AB ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were tehen incubated with a primary antibody solution containing BrdU antibody diluted 1:400 or 1:1000 in PBS containing 5% goat serum (Abcam, catalog # ab7481) and 0.1% Triton-X for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... iPS cells were treated for 1 h with HDR enhancer (5 μM; IDT, Coralville, USA) and 10 μM Y-27632 (Abcam, Cambridge, UK). Electroporated cells were seeded on Laminin 521 (Biolamina ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were incubated in one of the following primary antibodies in blocking solution containing 5% normal serum at 4°C overnight: F-actin (1:1000; Abcam, Cambridge, UK), mTOR (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% skim milk for 1 hour at RT before immunoblotting with antibodies against ACC1 (#ab72046, Abcam, 1:2000), ACC2 (#HPA006554 ...
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bioRxiv - Plant Biology 2023Quote: Immunoblotting was performed with antibodies diluted in the blocking solution (5% nonfat milk in TBS with 0.1% [v/v] Tween) at the following dilutions: α-GFP antibody (ab290, Abcam, Cambridge, UK), 1:8,000 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed three times with TBST for 5-min and then incubated for 1-hr with horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721, Abcam, Cambridge, MA). Membranes were washed three more times with TBST for 5-min.
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bioRxiv - Neuroscience 2024Quote: ... membranes were washed three times with TBST for 5-min and then incubated for 1-hr with horseradish peroxidase-conjugated goat anti-rabbit IgG (ab6721, Abcam, Cambridge, MA). Membranes were washed three more times with TBST for 5-min.
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed three times for 5 minutes each with PBS and then stained with a 1:3,000 dilution of Phalloidin-555 Alexafluor (# ab176756, Abcam, Cambridge, United Kingdom) in 300 µl PBS for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Bioengineering 2023Quote: ... which was prepared by 5% milk in TBS-T containing 1:10,000 horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibodies (Abcam ab6789-1 MG). The secondary antibody incubation was applied for 1 hour at room temperature ...
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bioRxiv - Genetics 2023Quote: ... was washed four times with 1X DPBS supplemented with 5 mg/mL BSA and subsequently crosslinked to 5 μg antibody (Anti-FOXA1 antibody: GeneTex, GTX100308; Anti-Fra2 antibody: Cell Signaling Technology, 19967S; Anti-H3K9me3 antibody: abcam, ab8898 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... The membrane was then treated with a blocking solution for half an hour (5% skim milk in 1% TBST) and incubated with the primary antibody (ab138501, Abcam, Cambridge, UK) at a ratio of 1:5,000 overnight at 4°C under constant motion ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed three times in 1XPBS for 5’ and incubated with anti-neural/glial antigen 2 (NG-2, 1:100, in 1XPBS + 1%NGS, Abcam, Cambidge, UK), overnight at 4°C.
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bioRxiv - Pathology 2024Quote: ... was performed on 5 μm sections according to the previously reported protocol (Sellmer Ramos et al., 2023; ab108422; 1:500 dilution; Abcam; Waltham, MA) to identify adenomyotic foci in uterine cross-sections ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed with PBS 3x 5 minutes and incubated with secondary antibodies (goad anti-rabbit 488 or 555; Abcam, 1:1000) 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... for 1h followed by antibody incubation in 5% Bovine Serum Albumin (BSA) in TBS-T overnight (TfR, 13-6800, ThermoFisher, 1:250; ApoE, Ab947, abcam, 1:500). After three times washing in TBS-T ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were probed with primary antibodies against PDK1 (1:2 500 in 5% skim milk/TBS-T, Cat. No. ab202468, Abcam, Cambridge, UK), β-tubulin (1:2 000 in 5% skim milk/TBS-T ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-HA (16B12, Biolegends, 2.25 µg/60 µl beads/IP) or anti-Ago2 (2E12-1C9, Abcam, 5 µg/60 µl beads/IP). Lysates were added to the beads and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Neuroscience 2021Quote: ... Mounted sections were dehydrated in 100% propylene glycol for 5 min and then stained with Oil Red O (ORO) for 10 min (Abcam, Cat. No. ab150678). The sections were then differentiated in 85% propylene glycol for 3 min and rinsed with distilled water three times ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then blocked (5% milk in TBS-Tween buffer) and incubated for 18 h with primary antibodies: CD44 (Abcam, 1:1000 dilution); GFAP (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were then permeabilized with 0.2% Triton X for 30 minutes at room temperature followed by blocking with 5% normal donkey serum (Abcam plc, Cambridge, MA, USA) and 300 mmol/L glycine for one hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies: mouse anti-HSP70 (ab2787, Abcam, Cambridge, MA, USA), rabbit anti-TSG101 (14497-1-AP ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were blocked with 5% BSA and then incubated with the antibody solution (anti-pMLKL 1:200, Abcam; anti-Iba1 1:700, Wako), overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam) ...
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bioRxiv - Neuroscience 2021Quote: ... brains were incubated in primary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Chicken anti-GFP (Abcam ab13970; 1:1000); anti-TH mouse monoclonal (SIGMA MAB318 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then probed to determine protein levels using the following antibodies diluted in 5% non-fat milk in 1x TBST: matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1:1000) and MMP2 (Abcam ab92536 ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated in blocking buffer containing (TBST+5% BSA) for 1hour at RT and incubated with primary antibody in blocking buffer at 4°C overnight: POLD1 (Abcam Cat# ab168827,1:500); GAPDH (Santa Cruz Biotechnology Cat# sc-32233 ...
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bioRxiv - Bioengineering 2020Quote: ... and PBS (twice, 5 min each) and left for 1 hour in block solution containing 10% goat serum (ab7481, Abcam, Cambridge, United Kingdom), 0.25% Triton-X 100 (Sigma Aldrich) ...