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Citations for abcam :
951 - 1000 of 1242 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed twice with PBS containing 0.025% triton-x for 5 minutes and then blocked with protein block (ab64226, Abcam) for 20 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was blocked with 5% (w/v) BSA in PBST overnight at 4°C and probed with anti-Huntingtin (1:1,000, ab109115, Abcam), anti-Ubiquitin (1:200 ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with TBST pH 7.6 for 5 min and incubated overnight at 4 °C in primary antibody (Mouse-anti-myc [Abcam #ab32 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with PBST (Triton 0.3 %) solution containing 5 % donkey normal serum and one or more antibodies (goat Anti GFP, ab6673, Abcam, diluted 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20) and incubated overnight with primary antibodies diluted in 5% milk in TBST (anti-ELK1 (Abcam ab32106) 1:500 ...
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bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in 0.3% PBT and incubated with secondary antibodies and Hoechst (Abcam) in 3% NDS in 0.3% PBT for 1 h at RT ...
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bioRxiv - Biophysics 2024Quote: ... The membrane was blocked in 5% milk in TBS-T buffer and probed with anti-CPEB4 antibody (Abcam ab224162).
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bioRxiv - Neuroscience 2024Quote: ... All chambers were again washed twice with PBS before a blocking solution consisting of 5 % goat serum (Abcam, ab7481) diluted in PBS was added to the cultures ...
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bioRxiv - Neuroscience 2023Quote: ... recovery aCSF included (R,S)-3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP, 5 μM; Abcam, ab120160) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNAs were fragmented into about 100-nt fragments and immunoprecipitated (IP) with 5 μg m6A antibody (Abcam, Cambridge, UK). The antibody-RNA complex was isolated by incubation with protein A beads (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: Free-floating sections were washed in PBS and blocked with 5% normal donkey serum (ab7475, Abcam, Cambridge, United Kingdom) in PBS containing 0.1% Triton X-100 (PBS-T ...
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bioRxiv - Pathology 2023Quote: ... and then incubated for 1 hour in PB solution containing 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). The sections were then incubated with the primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked with 5% milk in 0.1M TBST and probed with primary antibody: GAPDH (Mouse, Abcam G8795,1:1000) was used as an internal control for the concentration of protein loaded ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated with the primary antibody diluted in PBS containing 0.5% Tween-20 and 5% NGS (rabbit anti-mCherry antibody, Abcam, ab167453 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C with the following primary antibodies diluted in 0.2% sodium azide+5% BSA in 1X PBS : Sortilin-related receptor 1 (SORLA) at 1:1000 (# ab190684; abcam); Mouse monoclonal anti-Actin (clone A4 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was then washed (3 x 5 min) in TBST and incubated in secondary antibody (1:5,000, goat anti-rabbit HRP-conjugated secondary, Abcam) in 1% non-fat milk in TBST for 3 hrs at room temperature ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Cells were incubated in antibody binding solution (5% NGS and 0.1% Triton X-100 in D-PBS) containing LaminB1 (Abcam) or SON (Atlas Antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was performed over night at 4°C in a humidified chamber and the antibodies were diluted in antibody buffer (5% normal goat serum, PBS, 0.2% Triton X-100) (primary antibodies: Rabbit s100b, abcam, AB52642-1001 ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were subsequently incubated overnight at 4°C in 5% skim milk with primary antibodies (GPx4 (Abcam, ab125066), VDAC1/3 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... 5 µg/ml rat anti-mouse C3b/iC3b (clone 3/26, Hycult Biotec) and 5 µg/ml rabbit anti-mouse C1q (clone 4.8, Abcam). Secondary antibodies were added for 3h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated at 37°C for 30 min with complete DMEM containing 5 µM Zinpyr-1 (Abcam, ab145349), Hoechst 33342 (0.2 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Calcium transients were observed by incubating cerebral organoids for an hour with 8 µM Fluo-5 AM (Abcam; ab241083) in the ACSF ...
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bioRxiv - Biochemistry 2024Quote: ... before 5x 5 min washing (∼10 mL in TBS-T) and incubation with secondary Rabbit-HRP antibodies (ab79773, Abcam) for 60 min ...
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bioRxiv - Genetics 2024Quote: ... Standard 5 µm sections were prepared and stained for eosinophils (Anti-Ribonuclease 3/eosinophil cationic protein antibody, Abcam, Ab207429). The presence of ECRSwNP was determined by counting the number of eosinophils per high-power field (HPF) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were blocked in blocking buffer (5% bovine serum albumin in PBS) for 1 hour and incubated with the primary antibody RAD51 (ab133534, Abcam) or phosphor-H2AX (#05-636 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μl diluted cross-linked chromatin was incubated overnight with 5 μg mouse monoclonal anti-TEAD4 antibody (Abcam, Cat. #ab58310), 5 μg mouse monoclonal anti-YAP antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were blocked in 20% NGS for 30 min (Ki67) or 5% NGS for 60 min (CC3) before incubation in primary antibody overnight (anti-Ki67, Abcam; anti-cleaved Caspase 3 ...
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bioRxiv - Cell Biology 2020Quote: ... Fixed and permeabilized cells were then incubated with 1:400 dilution of Alexa Fluor 647 conjugated mouse anti-human cytokeratin 5 antibody (Abcam) for 24 hours at 4C in permeabilization buffer ...
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bioRxiv - Genetics 2021Quote: ... blocked with PBS/5% goat serum, and then incubated with primary antibodies: Mobp (1/1000, rabbit, Abgent) and Cnp (1/1000 mouse, Abcam) prior to exposure to fluorescent Alexa secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated for 10 min at 37 °C in the presence of 10 µM 2-amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile (UCPH-101; Abcam 120309, UK), a specific excitatory amino acid transporter 1 (EAAT1/Glast ...
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bioRxiv - Microbiology 2022Quote: ... and incubated overnight with primary antibodies diluted in 5% BSA: rabbit anti-PCBP1 (clone EPR11055, Abcam ab168378, diluted 1:10,000); rabbit anti-actin (A2066 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed 8×5 min in PBS before being mounted onto glass slides and cover-slipped with fluoroshield mounting medium with DAPI (AbCam). Dual staining was performed with sequential primary-secondary incubations ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed by incubation of 2 ml diluted chromatin (equivalent to 5×106 input cells) with antibodies for H3K4me1 (3 µg ab195391, lot: GR304893-2; AbCam), H3K4me3 (1 µl 07-473 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were blocked in 5% foetal bovine serum (FBS) for 30 min and incubated overnight in rabbit anti-PSD-95 (1:100; Abcam, Cambridge ...
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A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studiesbioRxiv - Cancer Biology 2020Quote: Formalin fixed paraffin-embedded sections (5 μm) were stained as described previously34 using the following primary antibodies: rabbit anti-Ki67 (ab15580, Abcam), rabbit anti-OLIG2 (ab109186 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes incubated with the primary antibodies against claudin-5 (1:1000, Zymed 1600-34) or GAPDH (1:400, ab181602, ABCAM)) and the appropriate secondary antibodies.
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bioRxiv - Microbiology 2021Quote: Clonal lines were screened for knockout by labeling with primary mouse monoclonal antibodies to CD59 (clone MEM-43/5; Abcam) or CD46 (clone C-10 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked with 5% BSA in TBST then probed with primary antibodies for HMGCR (Ms mAb, 1:000, abcam), MVD (1:1000 ...
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked with 5% non-fat skim milk and immunoblots were performed with indicated primary antibodies [anti-SUMO1 (Abcam), anti-Actin C3 (Abiocode) ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed and then incubated at RT for 1 h with horseradish-peroxidase (HRP)-conjugated goat anti-mouse (IgG) polyclonal antibody as the secondary antibody (1:5 000; Abcam). After three washes ...
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bioRxiv - Cell Biology 2020Quote: ... and heated to 95 °C for 5 min (no heating of the samples used for the total oxphos (abcam, ab110413) blots) ...
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bioRxiv - Neuroscience 2020Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in TBS + 2% BSA + 0.1% Triton X-100 at 5 µg/mL (rabbit anti-OCT4, Abcam #ab19857), 1:200 (mouse anti-NANOG ...
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bioRxiv - Cell Biology 2022Quote: ... paraffin slices were blocked for 1 hour at 37°C with 5% BSA before being treated overnight at 4°C with various primary antibodies as follows: anti-GFAP (Abcam), anti-CD68 (Abcam) ...
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bioRxiv - Physiology 2022Quote: ... blocked in 5% goat serum for 1 hour and stained using myelin protein zero (MPZ) (1 in 50 in 5% goat serum in PBS containing 1% triton) (EPR20383, Abcam) overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed with 1xPBS 3x for 5 minutes before incubation in 50 µl spots of secondary antibodies (goat anti-mouse AF647: Abcam ab150115 ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were permeabilized with 0.1% Triton X 100 in 1X PBS for 5 min and blocked with Protein Block (Abcam, USA) for 20 min at room temperature ...