Labshake search
Citations for abcam :
1001 - 1050 of 1242 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Samples were blocked with 5% normal goat serum for 1 hr and incubated in anti-TRPM4 (Abcam, catalog no. ab106200) or normal mouse IgG (Santa Cruz Biotechnology ...
-
bioRxiv - Immunology 2021Quote: ... 1×106 cells were blocked with DPBS with 5% of normal mouse serum (cat. n° ab7486, Abcam, Cambridge, MA, USA) for 30 min at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.25 x 106 cells were seeded into 10 mL cell culture dishes then treated with 5 ng/mL TGFbeta (Abcam) on the next day ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBS-T with 5% skim milk powder at 4°C overnight and incubated in 1:8000 diluted anti-GFP antibody (Ab290; Abcam) in TBS-T with 5% skim milk powder for one hour at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
-
bioRxiv - Systems Biology 2021Quote: ... All primary and secondary antibodies were diluted 1:400 in PBS containing either 2.25% bovine serum albumin [Millipore Sigma #A2058] or 5% normal goat serum (Abcam #ab7481) for blocking and 0.15% Triton X-100 (Sigma Aldritch #T8787 ...
-
bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 5 μL FLAG tag antibody (Anti-DDDDK-PE, Abcam ab72469, RRID: AB_1268475, or Anti-DDDDK-APC, Abcam ab72569, RRID: AB_1310127) was added at a concentration of 0.5 μg per sample and cells incubated at 4°C for 30 min ...
-
bioRxiv - Pathology 2021Quote: ... The antigen was retrieved by incubating tissue sections for 5-7 minutes in Proteinase K antigen retrieval solution (Abcam, ab64220). Incubation with primary monoclonal antibodies against HCN1 (Novus Biologicals ...
-
bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was incubated with 5 μl of ssDNA (10mg/ml) and 2 µl of mouse monoclonal anti-V5 antibody (Abcam), 4 µl of rabbit polyclonal anti-PrA (Sigma ...
-
bioRxiv - Immunology 2020Quote: ... Sections were blocked with protein block for 5 min and incubated overnight at 4°C with either rabbit polyclonal anti-CD3 antibody (1:50 dilution, ab5690, Abcam) or rabbit monoclonal negative control anti-IgG (1:50 dilution ...
-
bioRxiv - Biochemistry 2021Quote: ... coli were purchased and added to Kinase Buffer I (25 mM MOPS pH7.2, 12.5 mM β-glycerol phosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA, 0.25 mM DTT; Abcam ab189135). Either water (mock ...
-
bioRxiv - Immunology 2020Quote: ... serum was diluted 1:5 in diluent buffer and run in duplicate using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions.
-
bioRxiv - Physiology 2021Quote: ... Membranes were blocked in TBS-Tween 20 (0.1%) (TBST) with 5% BSA and then incubated overnight with primary antibodies for OXPHOS (Abcam MS604), P-RPS6 Ser235/236 (Cell Signaling ...
-
bioRxiv - Cancer Biology 2021Quote: ... tissue sections were blocked with 5% mouse serum PBS1X for 1h30 and incubated with mouse anti-tryptase antibody (1:300, ab2378, RRID:AB_303023, ABcam, Cambridge, UK) for 1h at RT ...
-
bioRxiv - Immunology 2020Quote: ... thawed unfixed tissue slices were rinsed with PBS then blocked with blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) for 1 hour at room temperature before incubation of mAbs at 5 µg/ml overnight at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... ABCA1 (ATP-binding cassette lipid transporter marker of multivesicular bodies; mouse anti-human ABCA1; 5 μg/ml; Abcam Cat. #ab18180) and ...
-
bioRxiv - Cell Biology 2020Quote: Exosomes (25 μg prot) from A-375 cells were incubated or not with 5 µl anti-CD63 antibodies (Abcam, ab8219) for 2 h at room temperature ...
-
bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant fluid was added to 25 µL of pre-incubated protein G magnetic beads (ChIP-Adembeads ChIP-Adem-Kit, Ademtech SA) with 5 µg of anti-V5 rabbit polyclonal antibody (ab9116, Abcam) in IP buffer (50 mM HEPES-KOH pH 7.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... 10% DMSO) and put into primary antibody (PTwH, 5% DMSO, 3% donkey serum, Goat anti-GFP, Abcam ab5450, 1:1000) for 10 days at 37 degrees Celsius ...
-
bioRxiv - Microbiology 2022Quote: ... blocked for 1 h using 5% donkey serum and then incubated with a rabbit monoclonal H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) at 1:500 dilution overnight in a humidified chamber at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Tissue sections were rehydrated with PBS for 5 minutes before blocking and permeabilization for 1 hour with 10% normal goat serum (Abcam) containing 0.3% PBS-TritonX-100 ...
-
bioRxiv - Bioengineering 2023Quote: ... was used to separately conjugate an anti-tau antibody (Tau-5, AB_2721194) and anti-α-synuclein antibody (MJFR1, Abcam, ab138501) to dibenzocyclooctyne (DIBO ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% donkey serum in 1x PBS for 1 hour and incubated with UFM1 (Abcam 1:100) and MC1 (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... Subsequently the membrane was incubated with the rabbit anti-IMPA1 antibody (1:10000 diluted in 5% BSA in 0.1% TBST) (Abcam ab184165) or goat anti-CHRM1 antibody (1 µg/ ml ...
-
bioRxiv - Cell Biology 2023Quote: ... were incubated for 6 hours on a rotating wheel at 4°C with primary antibodies MTHFD2 (Abcam; #ab151447; 5 μg) or negative control IgG (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... washed three times with PBS for 5 min each and incubated with secondary antibody Alexa Fluor® 647 (Abcam, ab150067) for an hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... The aCSF included 2,3-Dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX, Alomone Labs) 20 μM and 2-amino-5-phosphonopentanoate (D-AP5, Abcam) 50 μM to block glutamatergic AMPA and NMDA currents ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked using TBS-T with 5% BSA for 1 hour at room temperature and incubated using p-SMAD3 (ab52903, Abcam), type I collagen (1310-30 ...
-
bioRxiv - Microbiology 2023Quote: ... and 12.5 uL (12.5 ug) of MHC class II antibodies in a 3:1:1 mixture of TAL-1B5 (Abcam, ab20181), EPR11226 (Abcam ...
-
bioRxiv - Neuroscience 2023Quote: D-AP5 (D-(−)-2-Amino-5-phosphonopentanoic acid) and SR 95531 (2-(3-Carboxypropyl)-3-amino-6-(4 methoxyphenyl) pyridazinium bromide) were purchased from Abcam, UK ...
-
bioRxiv - Genomics 2023Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 μg/μL of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were incubated with the following primary antibodies in 5% skim milk at 4 °C overnight: rabbit anti-ATF4 antibody (ab270980, 1:1000, Abcam), XBP1 antibody (ab37152 ...
-
bioRxiv - Neuroscience 2022Quote: ... They were then blocked with rotation at room temperature for an hour in blocking buffer (5% normal goat serum (Abcam), 0.2% TritonX-100 PBS) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed 5 times with 1× PBST and incubated with goat anti-mouse horseradish peroxidase-conjugated secondary antibody (Abcam) for 30 mins then visualized using the chemiluminescence kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... Paraffin-embedded tissue was sectioned (5 µm) and stained with control IgG or ANO1 antibodies (Abcam # ab53212, 3 µg/ml) by the University of Chicago Immunohistochemistry Core Facility.
-
bioRxiv - Cancer Biology 2023Quote: ... were sonicated and incubated overnight under rotation at 4 °C with 5 ug/ml of anti-RNAPII-Ser2p (Abcam, ab193468) or anti-IgG (Cell Signaling ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes blocked for 1 h at room temperature were probed overnight at 4 °C in 5% non-fat dry milk with 1:2,000 anti-CA (Abcam ab63917) or 1:5,000 anti-IN77 ...
-
bioRxiv - Molecular Biology 2024Quote: Cells were stained 3-4 days after editing with anti-Cytokeratin 5 antibody [EP1601Y] (Alexa Fluor® 647) (Abcam, ab193895), Rabbit IgG ...
-
bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
-
bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4⁰C on a plate rocker in a 5% NGS/0.1% PBS-Triton solution containing rabbit anti-cFos antibody (Abcam, ab190289) at 1:500 dilution ...
-
bioRxiv - Neuroscience 2024Quote: ... the membrane was washed in TBS-T three times for 5 minutes each and incubated with goat anti-mouse IgG HRP (1:5000, cat# AB205719, Abcam) in TBS-T for 1 h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]) ...
-
bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760, 1:1000; IMPDH2, Abcam ab129165 ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were then permeabilized in 1mL of 0.1% Triton X-100 in 1X PBS for 5 min at room temperature and incubated with anti-DDX5 primary antibody (rabbit, ab21696; Abcam) diluted to 1:400 in PBS 1X for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... sections were blocked with 10% goat serum and then stained with Keratin 5 or Keratin 8 together with GFP antibody (Abcam) in 10% goat serum for overnight at 4□ ...
-
bioRxiv - Pathology 2023Quote: ... Sections were washed 3 times for 5-10 min in PBT and incubated for 2 h at RT in secondary antibody and Hoechst (Abcam) in 3% NDS in PBT ...
-
bioRxiv - Physiology 2023Quote: ... This was followed by placing in 5% normal goat serum for 1h and labelling for mCherry with rabbit anti-mCherry (1:10,000; Abcam; RRID:AB_2571870) and goat anti-rabbit 568 (1;400 ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 1 h prior to incubation in primary antibody in TBST with 5% skim-milk (rabbit anti-GFP 1:10,000; Abcam - ab290) overnight ...