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Citations for abcam :
1051 - 1100 of 1242 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Following imaging for loading control the membrane was blocked in 5% BSA in TBS-T and incubated overnight at 4 °C using N6-methyladenosine antibody (ab151230, Abcam). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... Slides were washed in TBS (3 x 5 min) prior to incubation with biotinylated goat anti-rabbit antibodies (Abcam, ab6720) in NGS (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antigen retrieval was accomplished by a 5-minute pressure cook of the slide submerged in 1X HIER buffer (abcam ab208572). Deionized water was slowly added to the pressure-cooked slide in HIER buffer ...
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bioRxiv - Biochemistry 2023Quote: ... samples were washed with 5 mL of 0.1% Triton X-100/PBS and incubated with 1 μL of IRF5 antibody (Abcam, 181553) in 100 μL of 0.1% Triton X-100/PBS for 2 h at RT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The slices were blocked with 5% goat serum (Beyotime, China) for 30 min and then incubated with anti-Aβ1-42 (1:300, Abcam, UK), anti-Tau(pSer396 ...
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bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
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bioRxiv - Pathology 2024Quote: ... paraffin-embedded 5 µm slices were stained for insulin (ab195956, 1:1500, Alexa Fluor 555-conjugated secondary antibody: ab150186, 1:500, Abcam) and glucagon (ab92517 ...
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bioRxiv - Neuroscience 2022Quote: Immunohistochemistry was performed for neuroblast detection and analysis of hippocampal neurogenesis using the following primary antibodies diluted in PBS containing 5% goat serum and 0.1% Triton-X-100: anti-doublecortin (DCX, rabbit: 1:5000, Abcam, #ab18723), anti-bromodeoxyuridine (BrdU ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used with a total of 5 µg of each primary antibody used per immunoprecipitation: Rabbit anti-LSD1 (ab17721, Abcam), Rabbit ani-MYC (13987 ...
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bioRxiv - Cell Biology 2022Quote: ... Anti-Cullin 5(ab184177), anti-Elongin B antibody (ab154854, 1:2000) and anti-SOCS2 antibody (ab109245, 1:1000) were purchased from Abcam. Antibody binding was visualized with peroxidase-conjugated sheep anti-rabbit immunoglobulin (Southern Biotech ...
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Evaluation of the DBA/2J mouse as a potential background strain for genetic models of cardiomyopathybioRxiv - Physiology 2022Quote: ... blocked in 5% BSA-PBS at room temperature and incubated with the anti-syntrophin primary antibody (1:2000; #11425; Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Tween-20 and 5% (w/v) milk powder containing 1:5,000 horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (AbCam ab205719) and incubated on a shaking platform for 1 hr at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 0.5 µm) were prepared and stained with 1% (w/v) toluidine blue and the Periodic Acid Schiff kit (ab150680, Abcam) as described in Hawkins et al ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was assessed after 72 hours using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, ab197010) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were blocked with 5% milk/TBST at room temperature for 1 hr before being incubated overnight at 4°C with paxillin (Abcam) and GAPDH (Cell Signaling Technology ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 μL of Annexin V-FITC and 5 μL of propidium iodide were added into each tube (Annexin V-FITC Apoptosis Staining/ Detection Kit, Abcam). The tubes were incubated at RT for 5 min in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1hr in secondary antibody diluted in TBST-5% milk (1:4000 ab6721 Goat Anti-Rabbit IgG H&L HRP (Abcam), 1:4000 ab205719 Goat Anti-Mouse IgG H&L HRP (Abcam)) ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Paraffin sections (5 μm) from lung tissues were processed using standard protocols for immunohistochemistry (IHC) staining according to manufacturer’s instructions (Abcam: ab236468). Tissues were permeabilized by incubation in 0.4% Triton-X in TBS for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... was detected in A549 cells fixed with 1% paraformaldehyde for 5 min using an anti-MT1-MMP rabbit monoclonal antibody (1:100 dilution, EP1264Y, Abcam). F-actin was stained with Alexa Fluor 350- or 568-conjugated phalloidin (Molecular Probes ...
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bioRxiv - Microbiology 2024Quote: ... Serine racemase treatment of human serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated with 20 μM NE for 5 min and an aliquot of saline was used to measure adenosine using an adenosine assay kit (Abcam) and a microplate reader (Perkin Elmer Enspire ...
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bioRxiv - Molecular Biology 2024Quote: ... Blots were subsequently washed 3 times for 5 minutes each in PBST at room temperature before incubation in 5µg/mL of HNRNPA2B1 polyclonal primary antibody (abcam 31645) in PBST overnight at 4°C with agitation ...
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bioRxiv - Physiology 2024Quote: ... Sections were placed in 100% propylene glycol for 5 minutes then incubated in pre-heated Oil Red O (Abcam #150678) for 6 minutes at 60°C ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was incubated with the primary antibody (RPS3, abcam, Cat#ab128995; eIF4E, abcam, Cat#ab33768; ORF3a, abcam, Cat#280953; 5-mC, Cat#ab214727; IFN-beta, abcam, Cat#ab275580 ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699 ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299; ERK1/2, Abcam, ab184699; phospho-CREB, Abcam, ab32096 ...
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bioRxiv - Microbiology 2024Quote: ... unskimmed milk powder, and incubated with mouse monoclonal anti-HA (clone 6E2, Cell Signalling Technology) and anti-p24 (clone 5, Abcam) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: The proliferation of CD3+ T-cell was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Abcam, US) following the manufacturer’s protocol [11] ...
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bioRxiv - Microbiology 2024Quote: ... To test for O-x-AlkTDM presence we used in-gel fluorescence (to detect TAMRA) and/or immunoblotted with anti-biotin antibodies (1:1000; 5% nonfat Milk in TBST; Abcam).
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bioRxiv - Microbiology 2024Quote: Anti-HIV-1 Pr55Gag + p24 + p17 antibody (ab63917) and anti-tubulin antibodies (B-5-1-2) were purchased from Abcam and Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... ∼50 µg of lysate material was treated with the reducing agent TCEP at a final concentration of 5 mM and heated to 60 °C for 30 minutes before being loaded onto a FASP protein digestion column (Abcam)75 ...
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bioRxiv - Immunology 2024Quote: ... Cells were permeabilized with 0.1% Triton X-100 / 2% BSA in 1x PBS for 5 min and F-actin was stained by Phalloidin-iFluor488 (1:1,000, Abcam #ab176753) for 1h room temperature prior to slides mounting (Fluoromount/DAPI).
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bioRxiv - Immunology 2024Quote: ... Slides were washed 3 x 5 min and stained with 1:1 HRP-conjugated goat anti-rabbit IgG (Abcam ab214880) in 1% BSA-TBS for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were blocked with 5% milk followed by incubation with primary antibodies overnight using the following antibodies: anti-VEGF-A (Abcam), anti-BCL2 (Cell Signaling Technology) ...
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bioRxiv - Molecular Biology 2024Quote: ... Wells were again washed three times for 5 minutes with dPBS before incubating with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] ...
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bioRxiv - Cancer Biology 2024Quote: ... or with recombinant TGF-β at 10 ng/mL (stock concentration 5 ng/µL) or Batimastat at 30 µM (stock concentration 1 mM, BB-94/Abcam). After 48 hours (or 1 hour for SMAD activity tests) ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µl of 1 mM DCFDA dye in 1X buffer and 5 µl of 10 µM DiOC2 was added for ROS evaluation (ab113851, Abcam) and membrane depolarization (B34950 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed for 5 mins in PBS and mounted with mounting media containing DAPI (catalog number ab104139, Abcam) before imaging.
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bioRxiv - Bioengineering 2024Quote: ... the blots were washed with PBS supplemented with 0.1% Tween 20 four times for 5 min before staining with the secondary antibody (7076, Cell Signaling; ab216773, Abcam) in blocking buffer with 0.1% Tween 20 for 1h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... The membranes were blocked (5% milk (PanReac AppliChem, A0830)) and probed with the following antibodies: anti-β-Tubulin (Abcam, ab15568), anti-GAPDH (Proteintech ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed 3 times for 5 min with PBS before incubating with a secondary antibody (ab150080 and ab150157, Abcam) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... All of supernatant containing digested nuclear extract was transferred to a new tube and 500µl of Low IP buffer without additional NaCl or detergent was added together with 5-10µg of KAT7 antibody (Abcam #190908) or rabbit IgG (R&D Systems ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 minutes and then incubated for 1 hour at room temperature (RT) in NM95 primary antibody (ab190710 16 - Abcam) at 1:1500 in TBS ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was measured via metabolic activity using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, UK). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk-TBST for 1 hour under agitation and probed overnight for ALIX (1:1000, 186429, Abcam), Calnexin (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibody was washed 2x with PBS for 5 min per wash and cells were incubated with secondary antibodies (diluted in 0.5% BSA/PBS) at room temperature in the dark for 1 h at 1:1000 dilution (anti-rabbit Alexa488, Abcam ab150077 ...
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bioRxiv - Microbiology 2020Quote: The mutant library was screened by preparing a 1 × 10-6 dilution in LB medium and plating on LB agar containing 60 µg ml-1 X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Abcam) and 500 µM IPTG ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked in 5% milk for 1h followed by incubation with primary antibodies: c-Myc (Abcam ab185656; 1:1000), Fos (Cell Signaling 2250S ...