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Citations for Promega :
2751 - 2800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Mass Spectrometry Grade (Promega) at 30°C for 17 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 2mM DTT and 0.5μl trypsin (0.5μg/μl, Promega, #V5111)) per sample for 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Proliferation was measured seeding 1,000 cells per well in a 96-well plate with CellTiter Blue (Promega,cat. # G8081) over the indicated time period and a plate reader (Perkin Elmer Victor X5 ...
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bioRxiv - Cell Biology 2024Quote: ... cDNAs were amplified with 6 PCR cycles using short Solexa P3/5 primers to allow beads-based size selection with ProNex Beads Size-selection Chemistry (Promega). In order to avoid over-amplification ...
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bioRxiv - Genetics 2024Quote: DNA template was generated via pGEM T-easy cloning system (Promega, A1360), where the insert was generated by PCR from 2 dpf total cDNA synthesized using iScript (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... Samples were digested by sequencing grade trypsin (Promega, V5111) in 50 mM ammonium bicarbonate at 37°C overnight ...
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bioRxiv - Genetics 2024Quote: ... cell lysates were prepared using the Dual-Glo Luciferase Kit (Promega) and luciferase luminescent activity measured with a Synergy H4 plate reader (Biotek) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Adult testis and ovary gDNA was extracted with the Wizard Genomic DNA Purification Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... gDNA was extracted from formalin fixed tissues with the ReliaPrep FFPE gDNA Miniprep System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using primers listed in Supplementary Table 5 and products cloned using the pGEM-T Easy Vector System I (Promega) or Phusion HF DNA polymerase (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... incubation with 0.5 μg of Trypsin/Lys-C Mix (Promega, Madison, WI) at room temperature for 3 h ...
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bioRxiv - Cell Biology 2024Quote: Add trypsin (V5280 Promega) to protein ratio was 1:20 and samples will be incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were subjected to trypsin (Promega, Madison, WI) digestion overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... for 20ms with 2 pulses or lipid-mediated delivery using FuGENE HD (Promega; TM328) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: Glycogen was measured using the Glycogen-Glo™ Assay (Promega Cat. #J5051) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... An additional 8 µl of cell lysate was transferred to a 384-well LV plate and ATP was measured by adding 8 µl CellTiter-Glo® Luminescent Cell Viability Assay (Promega Cat. #G7571).
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bioRxiv - Synthetic Biology 2024Quote: ... 10 µL reaction was treated with 0.2 mg/mL RNase A (Promega), 0.25 units RNase One (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... the GSH/GSSG-Glo™ Assay (Promega, reference V6612) was performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNase I (Promega) at 37 °C for 45 min and proceeded for library construction ...
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bioRxiv - Microbiology 2024Quote: ... pGEM3Zf(–) (Promega) was digested with HincII and phosphatase-treated ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were plated in a 24-well plate at 90,000 cells per well and transfected with gRNAs using Fugene HD (Promega) at a manufacturer-suggested 3:1 reagent:DNA ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day luciferase activity was measured using the Dual-Glo® Luciferase Assay System (Promega #E2920) and Infinite® 200 PRO microplate reader (TECAN #30190085).
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bioRxiv - Cancer Biology 2024Quote: ... and FuGENE 6 (Promega), at a 2:1 FuGENE:DNA ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... viability was quantified a luminescent system following manufactureŕs instructions (CellTiter- Glo, Promega). For Ba/F3 and FL5.12 lymphoma precursor cell lines ...
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bioRxiv - Cancer Biology 2024Quote: ... Urea was diluted to working 2 M concentration with 20 mM HEPES (pH 8.0) and tryptic (Promega) digestion performed at an enzyme to substrate ratio of 1:25 overnight with incubation at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Fugene HD transfection reagent (Promega; catalogue number: E231A) was used for transient transfections ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 ng renilla control reporter vector (Promega), mock luciferase ...
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bioRxiv - Cancer Biology 2024Quote: ... on a Glomax instrument (Promega). PPRE firefly activity was normalized to renilla activity and analyzed relative to vehicle controls.
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bioRxiv - Cancer Biology 2024Quote: ... HBEC cells were incubated for an additional 24 hours and then luciferase activity was measured using the Dual-Luciferase Reporter assay kit (Promega) on a Glomax instrument (Promega) ...
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bioRxiv - Immunology 2024Quote: ... and M-MLV reverse transcriptase enzyme (ref. M1708, Promega, Madison, WI, USA) at 42 °C for 60 minutes ...
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bioRxiv - Genetics 2024Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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bioRxiv - Developmental Biology 2024Quote: ... The secondary antibody used was HRP-conjugated goat/rabbit/donkey anti-mouse IgG (1 : 10000, Promega, W402B).
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bioRxiv - Developmental Biology 2024Quote: ... The 4 µg of linearized vectors were transcribed 5h using RiboMAX™ Large Scale T7 RNA polymerase kit (Promega, #P1300) with 5.6 mM Ribo m7G Cap Analog (Promega ...
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bioRxiv - Developmental Biology 2024Quote: ... with 5.6 mM Ribo m7G Cap Analog (Promega, #P1711) in reaction and resulting antisense probes purified with RNeasy Mini-Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: The Dual luciferase reporter assay kit (Promega, USA) was used to measure the reporter gene expression ...
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bioRxiv - Evolutionary Biology 2024Quote: ... this regulatory sequence was constructed into a pGL3-promoter vector (Promega), which contains the SV40 enhancer and firefly luciferase ...
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bioRxiv - Genomics 2024Quote: ... BrdU incorporation was detected by measuring absorbance at 450 nm using a GloMax plate reader (Promega).
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bioRxiv - Genomics 2024Quote: ... RNA was extracted with the Maxwell RSC Plant RNA kit (Promega, Madison, WI, USA) using the Maxwell® RSC device according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The ground material was used for genomic DNA extraction with the RSC Plant DNA Kit (Promega, Madison, WI, USA) using the Maxwell® RSC device according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was purified from 10-15 midguts using the ReliaPrep RNA Tissue Miniprep System (Promega). cDNA was made from 100 or 200 ng of RNA using PrimeScript RT Reagent Kit (TaKaRa) ...
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bioRxiv - Immunology 2024Quote: ... LDH activity was quantified in all cell supernatants using the Cytox96 non-radioactive cytotoxicity assay (Promega). Absorbances at 492 and 620 nm were measured with a Tecan Microplate Reader and reported as percentages of the value measured in the supernatant of cells treated with 1% triton for 10 minutes (total LDH).
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bioRxiv - Immunology 2024Quote: ... was first mixed with Fugene® (Promega) in a small volume of Opti-modified Eagle’s medium (MEM)™ reduced-serum medium (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... to a clear 96-well plate and fluorescence intensity measured at 520 nm excitation (Glomax plate reader; Promega). Fresh Presto blue solution was also read as a blank control ...
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bioRxiv - Synthetic Biology 2024Quote: ... and activity was measured with Luciferase Assay System (Promega, Madison, WI) using vendor-suggested procedures ...
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bioRxiv - Microbiology 2024Quote: ... DNA bands at ∼390bp were excised from gel and purified using Wizard™ SV Gel and PCR Cleanup System (Promega A9282) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Lanes containing proteins were excised in two bands and subjected to a manual in-gel digestion with modified porcine trypsin (Trypsin Gold, Promega). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... and PCR (Promega #M7806) using primers flanking the sequence targeted by the sgRNA (Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription reactions were performed by adding 0.5 µl (corresponding to 5 U) of AMV Reverse Transcriptase (Promega), 0.1 µl dNTP mix (10 mM) ...
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bioRxiv - Immunology 2024Quote: Cytokine or chemical inhibitor doses were selected based on available EC50 data and toxicity profiles with untreated macrophages (Trypan Blue staining or MTS assay; CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega, Madison, WI, USA). Only inhibitor doses resulting in >90% cell viability were utilized ...
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bioRxiv - Immunology 2024Quote: ... Triglyceride-Glo™ Assay and Cholesterol/Cholesterol Ester-Glo™ Assay kits (Promega; Madison, WI, USA) were used to quantify triglycerides and cholesterol ...