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Citations for Promega :
201 - 250 of 3827 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Luciferase activity of cell lysate transfected with pGL3 promoter constructs was detected with the Dual Luciferase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: CEBPa promoter (−2kb) or enhancer region (Cooper et al., 2015) was cloned into the pGL3 basic backbone (Promega). The construct containing part of the Id1 promoter region (336 bp ...
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bioRxiv - Developmental Biology 2021Quote: ... A 2kb DNA fragment upstream of TSS of Snail1 promoter (ENSGAL00000008018) was cloned into pGL3 basic plasmid (Promega) using PWO polymerase (Roche ...
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bioRxiv - Genomics 2020Quote: ... PCR oligonucleotides contained additional sequences complementary to Bgl II restriction endonuclease digested pGL3-promoter plasmid (Promega, Southampton, UK) for subsequently cloned using the InFusion HD Cloning Kit protocol (Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity of cell lysate transfected with pGL3 promoter constructs was detected with the Dual Luciferase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The Rosa26 promoter and the 5’UTRs of secretory granule protein transcripts were subcloned into the pNL1.1 (Promega) using standard cloning techniques ...
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bioRxiv - Molecular Biology 2022Quote: We generated a promoter luciferase reporter construct for elovl5b using the pGL4.10[luc2] vector (Promega, GenBank accession# AY738222), which contains the firefly luciferase reporter gene ...
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bioRxiv - Molecular Biology 2023Quote: ... pGL3basic-IFNβ-Luc (mIfnb1- FLuc) consists of the 812 bp murine Ifnb1 promoter region cloned into pGL3basic (Promega) upstream of the firefly luciferase gene (99) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 ng/μL LRP2 promoter-driven Renilla luciferase reporter (S712992, Switchgear Genomics) and 0.4 pg/μL PGL4.50 Photinus pyralis (E310, Promega) luciferase reporter were simultaneously treated with 10 nM 25D or 10 nM T ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Molecular Biology 2020Quote: ... whereas Bmal1-luc was constructed by inserting the mouse Bmal1 promoter (−779 to +127 in reference to the Bmal1 TSS) to pGL4.11[luc2P] (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... Per2E2-luc reporter gene was constructed by inserting the mouse Per2 promoter (−83 to +156 in reference to the Per2 TSS) into pGL4.12[luc2CP] (Promega), whereas Bmal1-luc was constructed by inserting the mouse Bmal1 promoter (−779 to +127 in reference to the Bmal1 TSS ...
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bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
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bioRxiv - Immunology 2022Quote: Putative regions for mouse FCER1A-S promoter and mouse FCER1A-AS promoter were synthesized by Sangon Biotech Company (Shanghai) and inserted into the multicloning sites of pGL-4.17 Basic (Promega) to generate reporter plasmids for detection of putative promoter activities ...
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bioRxiv - Developmental Biology 2022Quote: ... tropicalis Pax8-CNS1 sequence was removed from the 1pax8-pax8(-2038)-GFP construct (Ochi et al., 2012) with bamH1 and inserted upstream of the basal promoter of pGL4.23[luc2]miniP (PROMEGA) to generate pax8CNS1-luc ...
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bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector (Promega). Bovine genomic DNA was extracted from bovine chondrocytes and was used as the template to amplify Prg4 regulatory regions ...
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bioRxiv - Molecular Biology 2020Quote: Prg4 enhancer/promoter-firefly luciferase reporters were co-transfected together with the pGL4.75 [hRlu/CMV] renilla luciferase reporter (Promega) into either superficial zone chondrocytes ...
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bioRxiv - Genetics 2021Quote: ... we cloned human DNA sequences (Coriell) containing the reference or alternate allele upstream of the minimal promoter in the luciferase reporter vector pGL4.23 (Promega) in the forward direction using the restriction enzymes SacI and KpnI ...
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bioRxiv - Neuroscience 2021Quote: Luciferase reporter constructs were constructed by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
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bioRxiv - Genomics 2020Quote: ... we cloned human DNA sequences (Coriell) containing the reference allele upstream of the minimal promoter in the luciferase reporter vector pGL4.23 (Promega) using the enzymes Sac I and Kpn I ...
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bioRxiv - Genomics 2020Quote: ... Each well was transfected with 25ng of the corresponding putative enhancer-cloned pGL3-promoter luciferase reporter along with 1.5ng Renilla (pRL-TK Vector, Promega) control reporter vector using FuGENE HD transfection reagent as previously described [20] ...
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bioRxiv - Developmental Biology 2020Quote: ... the Satb1 enhancer sequence was cloned into the KpnI and XhoI sites of pGL4.25[luc2CP/minP] firefly luciferase vector containing a minimal TATA promoter (Promega). Rcho1 TS cells were used for the reporter assay ...
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bioRxiv - Neuroscience 2022Quote: Luciferase reporter constructs were generated by cloning a candidate enhancer region into the pGL3 promoter vector (Promega; Madison, WI). Each region was inserted using standard restriction enzyme-based cloning techniques ...
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bioRxiv - Developmental Biology 2024Quote: CSCs and cells from 9-day-old CBs were transiently transfected with the nkx2.5 promoter-reporter plasmid pNKE24 44 and with pGL3-Basic (E1751, Promega) and phRL-TK (E2241 ...
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bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labelled antisense and sense RNA probes were transcribed from T7 or SP6 promoter of pGEMT-Easy vector (Promega) according to manufacturer ‘s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... A 9.5 kb fragment containing upstream promoter region of mouse Frizzled10 (Fzd10) gene was subcloned into pGEM vector (Promega). CreERT2gene (Feil et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Digoxigenin-labelled antisense and sense RNA probes were transcribed from T7 or SP6 promoter of pGEMT-Easy vector (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... pGL3-Vλ1p was constructed by cloning the Vλ1 promoter (chr16: 19084509-19085159) in front of luciferase reporter gene in pGL3-Basic (Promega). To construct pGL3-Vλ1p-HSE-1 and pGL3-Vλ1p-HSCλ1 ...
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bioRxiv - Cell Biology 2023Quote: Mouse genetic Hnf4a promoter (-2000 to 0) and its deletion mutants (-350 to 0) were cloned into pGL4.14 vector (E6691, Promega) by Gibson Assembly kit (A46624 ...
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bioRxiv - Immunology 2023Quote: HIC1 binding on RUNX1 promoter was assessed using Dual-Luciferase® Reporter Assay by cloning the ChIP-defined genomic region upstream of a minimal promoter driving a luciferase gene (pGL4.10 [luc2/minP]; Promega). Wild type HIC1 binding motif of the RUNX1 proximal promoter (5′-TGCCCTGG-3′ ...
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bioRxiv - Molecular Biology 2023Quote: A construct bearing 2 kb of the murine Bigheart promoter (pGL3-B.HTSS2KB) was subcloned as a HindIII and SacI fragment into the pGL3-Basic vector (Promega). pGL3-Intron3-DSCR1 harbors the complete (875 bp ...
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bioRxiv - Cell Biology 2024Quote: ... 5 regions of ieCTNNB1 and the region containing the mutation site were respectively cloned into pGL3- promoter vector (Promega). HEK293T ...
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bioRxiv - Cancer Biology 2024Quote: Promoter sequences for the mouse Cdk4 and b2m genes were inserted into the firefly luciferase promoter fusion plasmid pGL4.10 (Promega). The Cdk4 and b2m promoters were obtained from mouse gDNA using oligonucleotides 5’-TGA-GCT-CGC-TAG-CGA-GGC-TAG-CTA-CGT-GTC-CAT-3’ and 5’-AGA-TCT-TGA-TAT-CCC-AGA-CCA-TAG-ACA-CAG-GCC-3’ (Cdk4 ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Microbiology 2021Quote: Promoter fusions to lacZ were constructed by amplification of promoter regions from genomic DNA with the specified primers (Table S4) and ligated into pGEM-T Easy (Promega) for amplification and collection ...
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bioRxiv - Developmental Biology 2021Quote: ... the proximal promoter region (−299/−1) of MyoG was isolated by PCR and inserted into the pGL3-basic vector (Promega). The DNA fragments of PME1 and PME2 in the MyoG promoter region were also amplified and inserted into the pGL3-γ-actin-TATA vector ...
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bioRxiv - Molecular Biology 2020Quote: The non-TOP 5′ UTR sequence of ATP5O (5′-CGGGAGAAG-3′) was identified in published nanoCAGE data 90 and inserted between the T7 promoter and Renilla luciferase in psiCHECK2 (Promega) by Gibson Assembly.
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bioRxiv - Genomics 2020Quote: ... The promoter activity was evaluated by measurement of the firefly luciferas e activity relative to the internal control minimal TK promoter driven-Renilla luciferas eactivity using the Dual Luciferase Assay System as described by the manufacturer (Promega). A minimum of four independent transfections was performed and all assays were replicated at least twice.
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bioRxiv - Molecular Biology 2021Quote: ... The GAL1 promoter was amplified from yeast genomic DNA using primers 4588-1/XH5103 and ligated into pGEM-Teasy (Promega) to create pCB4554 ...
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bioRxiv - Molecular Biology 2021Quote: The minimal CYC1 promoter with seven tetracycline binding sites was amplified from the yeast Tet-Promoters collection35 with YG4866/YG4867 and cloned into pGEM-Teasy (Promega) to create pCB4695 ...
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bioRxiv - Cell Biology 2020Quote: ... Generated WIP1 and WIP1-ΔκB promoter PCR products were cloned into pGL3-basic luciferase reporter vector (Promega, Madison, WI, USA) using XhoI and BglII restriction enzyme sites and designated as pGL3-WIP1 and pGL3-WIP1-ΔκB ...
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bioRxiv - Microbiology 2022Quote: Templates for in vitro transcription of gene-specific 300-500 nt dsRNA and DENV2 EMSA probes were generated by PCR introducing a T7 promoter sequence or a universal tag at both 5’ and 3’ ends using the GoTaq Flexi DNA Polymerase (Promega). If present ...
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bioRxiv - Cancer Biology 2022Quote: Nucleotides +50 to -755bp (relative to the transcriptional start site) of the larger FAM129B isoform were cloned into the pGL2basic luciferase promoter plasmid (Promega). The AP-1 mutant was constructed by changing the TRE site ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116 cells expressing the auxin-responsive F-box protein Oryza sativa TIR1 (OsTIR1) under the control of a Tet promoter were transfected using FuGENE HD (Promega) with a CRISPR/Cas9 plasmid targeting nearby the 1st ATG codon of the RIF1 gene (5’-TCTCCAACAGCGGCGCGAGGggg-3’ ...
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bioRxiv - Cancer Biology 2020Quote: ... were PCR amplified and cloned into either pGL3-Promoter firefly luciferase reporter vector or pGL3-Basic luciferase reporter vector (Promega). Constructs were verified by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: The 1-450bp DNA region of the luciferase coding sequence was synthesized with G/C content optimized and ‘ATG’ codons removed and then inserted into the pGL3-Promoter vector (E1761, Promega) in place of the original full-length luciferase coding region using ClonExpress®II One Step Cloning Kit (C112-01 ...
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bioRxiv - Cell Biology 2021Quote: The luciferase reporter plasmid was generated by cloning the mouse HDAC7 promoter into the NheI site of pGL4 Luciferase reporter plasmid (Promega). The mouse HDAC7 promoter was amplified using Platinum SuperFi II DNA Polymerase (12361010 ...