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Citations for Promega :
201 - 250 of 1170 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (66) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each gel plug was then digested with Trypsin Gold (Promega) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... In-Gel digestion was carried out using Trypsin gold (Promega). The samples were purified using C18 SepPak columns (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were saturated with 250 ng trypsin (Promega) in 50 μL 10 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (42) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then transfected with 1500 ng pcDNA-FLAG-HA-sscGAS plasmid complexed with FuGene 6 reagent (Promega) according to manufacturer’s instructions or treated with FuGene 6 alone as a negative control ...
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bioRxiv - Cell Biology 2020Quote: ... mutagenesis primers were used for PCR amplification of pRK7-FLAG-Rheb using pfu Turbo Polymerase (Promega, NSW, Australia) to induce specific mutations ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
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bioRxiv - Immunology 2021Quote: ... and HHEX (NM_002729) attached to a FLAG tag at the 5’ end and HiBit tag (Promega, Madison, WI) at the 3’ end ...
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bioRxiv - Genetics 2024Quote: ssDNA constructs generated by M13KO7 helper phage were run on a 1% agarose 1X TBE gel at 100 V for 45 min then size selected ssDNA purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega Madison, WI cat no. PR-A9281) and quantified via Nanodrop 6000 (Thermo-Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
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bioRxiv - Immunology 2020Quote: ... The following day the cells were transfected with plasmids encoding the indicated FLAG-tagged protein (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h cells were harvested and washed 3 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged CXCR2/CXCR3/CXCR7 and 3.5μg of F22 (Promega, Cat. no: E2301). 14-16h post-transfection ...
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bioRxiv - Genetics 2021Quote: ... RNA was visualized by incubating the gel in Diamond Dye (Promega) and the 20-29 nt area of the gel was cut out for purification ...
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bioRxiv - Pathology 2019Quote: ... following manufacturer’s instruction followed gel electrophoresis using 1 kb ladder (Promega). Sequencing reactions were performed by Fisher Scientific or MyGATC ...
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bioRxiv - Cell Biology 2019Quote: ... followed by in-gel digestion with 20ng/μl of trypsin (Promega) for 16-20 h (overnight ...
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bioRxiv - Biochemistry 2020Quote: ... before being in-gel digested using modified trypsin (Promega, sequencing grade) as previously described (Casabona et al. ...
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bioRxiv - Systems Biology 2021Quote: ... purified using Wizard SV Gel and PCR Clean-Up System (Promega), and all 4 reactions were pooled together ...
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bioRxiv - Microbiology 2020Quote: ... In-gel digestion was performed using sequencing-grade-modified trypsin (Promega) and incubated overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Cell Biology 2022Quote: ... in-gel tryptic digestion (250 ng Trypsin-Lys-C mix Promega V5072 in 50 mM ammonium bicarbonate overnight at 37oC) ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then in-gel digested using trypsin (sequencing grade, Promega). Resulting peptides were analyzed by online nanoLC-MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus or HF ...
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bioRxiv - Plant Biology 2021Quote: ... In-gel digestion was then carried out with trypsin (Promega, USA) in 50 mM ammonium bicarbonate (Solarbio ...
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bioRxiv - Immunology 2021Quote: ... before kit purification (SV Gel and PCR Clean up System, Promega) and ligation in presence of T4 ligase (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... PLK1 proteins isolated by gel electrophoresis were digested with trypsin (Promega) in 100 mM NH4HCO3 pH 8.0 ...
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bioRxiv - Physiology 2022Quote: ... The corresponding PPARγ2 band was cut for in gel Trypsin (Promega) digestion ...
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bioRxiv - Systems Biology 2023Quote: ... samples were in-gel digested with trypsin (sequencing grade, Promega, Mannheim), the resulting peptides extracted by two changes of 5% formic acid (FA ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing purity, Promega), as previously described [51] ...
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bioRxiv - Biochemistry 2023Quote: ... 5% polyacrylamide TBE gel and stained with Diamond DNA stain (Promega). Experiments were performed with H ...
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bioRxiv - Systems Biology 2023Quote: ... Gel fragments were rehydrated in 20 ng/µl trypsin (Promega, V5117) in 50 mM ammonium bicarbonate on ice for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described [88] ...
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bioRxiv - Molecular Biology 2023Quote: ... gel pieces were re-swollen in a 0.1µg/µl trypsin (Promega) solution (NH4HCO3100mM ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were in-gel digested using 1µg trypsin (Trypsin Gold, Promega)(37) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing grade, Promega), as previously described 58 ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification (Promega Wizard SV Gel and PCR clean-up system), the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... After purification (Promega Wizard SV Gel and PCR clean-up system), the concentration of the HRT was adjusted to 100 ng/µl as measured with Nanodrop (Thermo Scientific) ...
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bioRxiv - Physiology 2024Quote: ... followed by in-gel digestion with trypsin (Promega, Madison, WI, USA). The resulting peptides were extracted and analyzed using a nanoUPLC-system (nano-Acquity ...
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bioRxiv - Cell Biology 2021Quote: ... and Flag-tagged HGF WT and HGF 4Cys-4Ala were in-vitro translated (TNT quick coupled Transcription/Translation system, Promega) and were incubated with the bead bound c-MET for 4h at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2019Quote: ... After 24 hours cells were transfected with pcDNA3 plasmids containing ΔCTT STING-FLAG variants with or without co-transfection with pcDNA3 CTT-HA plasmids using Fugene 6 Transfection Regent (Promega). After 24 additional hours cells were lysed in mild detergent (10% glycerol 25mM NaCl ...
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bioRxiv - Genetics 2020Quote: ... 2 µg NFIA expression construct (NFIA-FLAG) was transfected to each well of cells by FuGENE HD Transfection Reagent (Promega). Nuclear extracts containing the NFIA protein were extracted from 3 wells of transfected cells using nuclear and cytoplasmic extraction kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected with Halo-FLAG-FUS plasmids using the FuGENE HD Transfection Reagent (Promega, Madison, WI, catalog #: E2311). The cells were cultured in a medium containing the ligand (10 μM ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Plant Biology 2023Quote: Myc- and FLAG-tagged versions of the different ALOG proteins were produced using the Quick Coupled Transcription/Translation System (TnT; Promega). 25 µL of TnT reactions producing indicated proteins were mixed with Buffer 1 to reach 150 µL and rotated at 4 °C during 1 h ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown to 80% confluency prior to transfection with the indicated FLAG tagged ISG15 pcDNA5 plasmids using the FuGene transfection reagent (Promega). After 24 hr ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...