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Citations for Promega :
451 - 500 of 1170 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (A9281, Promega) and the purified templates were used to produce single stranded digoxigenin (DIG)-labelled RNA probes with the T7 (P2075 ...
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bioRxiv - Immunology 2020Quote: ... The PCR fragment was cleaned up using the Wizard SV Gel and PCR Clean-up System (Promega). The fragment was digested with EcoRI-HF and HindIII-HF (New England Biolabs ...
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bioRxiv - Zoology 2021Quote: ... Those containing single bright bands were purified using Wizard SV Gel and PCR Clean-up Kit (Promega), and sequenced in both directions using PCR primers at Sequetech Inc ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was run on the agarose gel and purified using PCR purification kit (A1222, Promega). The Alu PCR product was cloned into using pGEM-T Easy Vector (A1380 ...
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bioRxiv - Biochemistry 2020Quote: ... Destained gel pieces were dried in vacuo and rehydrated by the 12.5 μg/ml trypsin (Promega, USA), 50 mM ABC ...
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bioRxiv - Molecular Biology 2019Quote: ... we purified PCR amplicons using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and sequenced purified PCR products with BigDye® Terminator v3.1 cycle sequencing kit (Applied Biosystems® ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were in-gel digested by addition of 200 µL of 2 ng/µl trypsin (Promega, V511A) in resuspension buffer (Promega ...
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bioRxiv - Genetics 2022Quote: ... Monosomal bands were cut and purified by Wizard SV Gel Clean-Up System Kit (Promega, REF A9282). DNA samples were diluted 1:30 and used in quantitative polymerase chain reactions (qPCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were purified using the Wizard PCR Cleanup and Gel Purification system (Promega, Fitchburg, WI, USA).
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplicons were purified using the Wizard® SV Gel and PCR Clean-Up System kits (Promega), followed by Sanger sequencing (Eurofins) ...
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bioRxiv - Developmental Biology 2023Quote: ... Dried gel samples were digested overnight with mass spectrometry grade trypsin (Trypsin Gold, Promega, Madison, WI, USA) at 5ng/μl in 50 mM NH4HCO3 digest buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Gel bands were washed in 100 mM AmBic/ACN prior to adding 1 µg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Gel bands were washed in 100 mM AmB/ACN prior to adding 1 µg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Genomics 2023Quote: ... Agar was removed from the DNA using the Wizard SV Gel and PCR Clean-Up System (Promega). Transposon-directed insertion site sequencing (TraDIS ...
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bioRxiv - Genetics 2022Quote: ... The digested plasmid was purified using the Wizard SV Gel and PCR Clean-Up System (Promega A9281). The zebrafish exorh promoter region was amplified from pCK029 (this manuscript ...
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bioRxiv - Biochemistry 2023Quote: ... prior to running on a denaturing urea 10% polyacrylamide gel and stained with Diamond DNA stain (Promega). The experiment was repeated in triplicate and quantified using BioRad Image Lab software.
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bioRxiv - Cell Biology 2023Quote: ... The inserts and plasmid backbones were purified with Promega Wizard SV gel and PCR Cleanup System (Promega). Purified inserts and backbones were mixed in a molar 3:1 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... and in-gel digestion with sequencing grade modified trypsin (Promega, 12.5 ng/μL in 50 mM NH4HCO3). Samples were analyzed with a LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The FgTad3-GFP band cut out from the SDS-PAGE gel was digested with trypsin (Promega, USA) in gel at 37°C for 12 hours ...
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bioRxiv - Plant Biology 2024Quote: ... The second PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega) and the Agencourt AMPure XP Kit (Beckman Coulter) ...
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bioRxiv - Microbiology 2024Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng of trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplified fragments were run on an agarose gel and purified using the Wizard SV kit (Promega). The sequences were cloned into the pUASz1.0 vector (54 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products (1.5Kb) of 16S rRNA gene were cleaned with Gel and PCR Clean-Up system (Promega, USA), and quantified by NenoDrop ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) kit ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) kit for Sanger sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel purified and sub-cloned into the pGEM®-T Easy Vector System (Promega) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... RNA concentration was measured by scanning the gel at fluorescence mode with a Typhoon 8600 scanner (Promega, USA). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Gel pieces were rehydrated in 10-15 µL of solution containing 20 ng/µL trypsin (Promega, Madison, WI) in 50 mM ammonium bicarbonate for 15 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... The bands were purified using the Wizard® Plus SV Gel and PCR Clean-Up System kit (Promega), using the Quick Protocol specified by the manufacturer ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: Eluted protein samples from BIA experiment were digested in gel with 1 µg of trypsin (sequence grade, Promega) at 37°C OVN ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The WGA products were purified using Wizard® SV Gels and PCR Clean-Up System (Promega, WI, USA). The DNA samples were used for library preparation ...
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bioRxiv - Bioengineering 2020Quote: ... Wizard Mini-Preps Plasmid DNA purification and SV Gel Clean-up kits were purchased from Promega (MyBio, Ireland). The O2-sensitive phosphorescent probe Pt-Glc was synthesized as described before [82] ...
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bioRxiv - Cell Biology 2021Quote: ... entire gel lanes were excised into 5 equal regions from top to bottom and digested with trypsin (Promega) as described (30) ...
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bioRxiv - Plant Biology 2021Quote: ... Deletion products that could not be resolved by agarose gels were cloned into pGem-T-easy vector (Promega) following the manufacturer’s protocol and individual clones were subject to Sanger sequencing ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were pooled and purified with Wizard Gel and PCR Clean-up Kit (Promega, Madison, Wisconsin, USA). Purified PCR products were sequenced at Michigan State University ...
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bioRxiv - Microbiology 2022Quote: ... After purification of the PCR product with Wizard® SV Gel and PCR Clean-Up System (Promega, UK), the arsM amplicon was sequenced at Microsynth (Balgach ...
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bioRxiv - Molecular Biology 2022Quote: ... and the amplified DNA fragments were purified using Wizard SV Gel and PCR Clean-Up System (Promega #A9282). DIG-labelled RNA probes were synthesized using T7 or SP6 RNA polymerase and DIG RNA labeling Mix (Roche #11277073910) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA products of correct sizes were gel-purified and subcloned into the pGEM-T Easy cloning vector (Promega). Selected clones were sequenced on an automatic DNA sequencer and aligned to GenBank (https://www.ncbi.nlm.nih.gov/genbank/ ...
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bioRxiv - Cancer Biology 2022Quote: ... Wizard® SV Gel and the PCR Clean-up System were used to clean the PCR products (Promega). Next ...
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bioRxiv - Genetics 2019Quote: ... and the linearized vector was purified by Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The pure linearized vector and the elpc-2 promoter were fused using an In-Fusion HD Cloning Kit (Takara ...
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bioRxiv - Genomics 2020Quote: ... Obtained products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA), and subsequently cloned using the pGEM-T Easy Vector System (Promega ...
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bioRxiv - Genetics 2019Quote: ... The PCR product was purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) according to manufacturer instructions.
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bioRxiv - Genomics 2021Quote: ... The three fragments were cleaned up using the Wizard® SV Gel and PCR Clean-Up System (Promega) and subsequently fused by overlapping PCR ...
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bioRxiv - Neuroscience 2021Quote: ... gel pieces were rehydrated with 10 µL 50 mM NH4HCO3 containing 25 µg/mL trypsin (Trypsin Gold, Promega) and incubated for 15 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... where the samples were destained and subjected to in-gel proteolytic digestion with trypsin/Lys-C mixture (Promega). The digests were extracted ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting amplicons were purified and concentrated using a Wizard SV gel purification kit (Promega Corporation, U.S.A.), and cloned into a P-Gem T plasmid (Promega Corporation ...
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bioRxiv - Microbiology 2020Quote: ... In-gel digestion was performed by adding 25 μL of 4 μg/mL sequencing grade modified trypsin (Promega) in 25 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... Dry gel pieces were digested with 200 μL of mass spectrometry-grade trypsin solution (4 ng/μL, Promega) in AMBIC and incubated at 37°C for 18 h ...