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Citations for Promega :
101 - 150 of 1170 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The PCR products and restriction enzyme digestions were purified by agarose gel electrophoresis followed by processing with the Wizard SV Gel and PCR clean up system (Promega). Restriction enzymes were purchased from Fermentas ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The PCR fragments were purified over gel and the DNA was recovered using the Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Plant Biology 2023Quote: ... 2D gels were stained by Coomassie brilliant blue and proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Zoology 2024Quote: ... The amplicons-containing were excised after gel-electrophoresis and extracted DNA by using Wizard SV Gel and PCR Clean-Up System (Promega). The amplicon DNAs were further analyzed for Sanger sequencing with the QuantumDye Terminator Cycle Sequencing Kit (Tomy Digital Biology ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were confirmed on a 0.8% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up kit (Promega). DNA fragments were quantified using Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: The denatured protein samples from co-immunoprecipitation were run into stacking gel and the sample gel band stained by Coomassie Brilliant Blue G250 were digested by trypsin (V5280, Promega) with prior reduction and alkylation in 50 mM ammonium bicarbonate at 37°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... The resultant PCR products were subsequently subjected to purification through agarose gel electrophoresis employing the Wizard Sv Gel Clean-up system (Promega). Following purification ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting products were checked on agarose gel (1%) and purified using the Wizard SV Gel and PCR Clean-Up System (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... proteins were in-gel digested with trypsin (Promega) and the resulting peptide mixtures were processed on STAGE tips (92 ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel extracted (Promega, A9281). Wildtype embryos were injected with a mix containing two sgRNAs (56.25 ng/μl each) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko 37 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko49 ...
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bioRxiv - Plant Biology 2021Quote: ... In-gel digest was performed with trypsin (Promega) in 50 mM ABC and 5 % ACN at 37 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... In-gel digestion using trypsin (Promega, Madison, WI) was performed as previously described74 ...
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bioRxiv - Biophysics 2022Quote: ... and subsequently purified by gel purification kit (Promega).
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko [46] ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko (Shevchenko et al. ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko78 ...
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bioRxiv - Cell Biology 2023Quote: ... and in-gel digested with either ArgC (Promega) overnight at 37°C or trypsin (Promega ...
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bioRxiv - Genetics 2023Quote: ... Gels were stained using Diamond DNA stain (Promega) and colloidal blue protein stain to confirm loading ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with Diamond DNA stain (Promega) and the disappearance of the NCP band quantified using ImageLab ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by in-gel digestion with trypsin (Promega) at 37°C for overnight ...
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were than extracted from agarose gels and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). Eluted DNA solutions were then precipitated with ethanol and resuspended in RNase-free water.
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bioRxiv - Biophysics 2022Quote: ... The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit). A set of oligonucleotides that form a biotinylated and primed fork was ligated to one end of the fragment and the final product purified on a Sepharose-4B column as previously described [42] ...
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bioRxiv - Immunology 2021Quote: ... Products were isolated from a 2% agarose gel using the Wizard1 SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany). DNA concentration was determined via the Qubit1 1.0 Fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: The protein bands of interest were excised precisely from SDS polyacrylamide gel and subjected to in-gel trypsin digestion using sequencing grade trypsin (Promega, USA). MALDI TOF was performed in HCT Ultra PTM Discovery System (ETD II-Bruker Daltonics ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was separated on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System (Promega, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The vector and digest were both purified using gel extraction using the Wizard® SV Gel and PCR Clean-Up System (Promega) and ligated using T4 ligase (NEB ...
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bioRxiv - Immunology 2022Quote: ... PCR products were run on a 1 % agarose gel and amplicons of the expected size were purified using the Wizard SV Gel and PCR Clean-Up System (Promega, A9282). The samples were submitted to Sanger Sequencing (sequencing service ...
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Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteriabioRxiv - Cell Biology 2019Quote: ... The resulting PCR products were extracted from 2% agarose gel and further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences) and were quantified by QuantiFluor™-ST (Promega).
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bioRxiv - Microbiology 2022Quote: ... DNA bands at ∼390bp were excised from gel and purified using Wizard™ SV Gel and PCR Cleanup System (Promega A9282) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The DNA gel bands were excised and purified with a Wizard® SV Gel and PCR Clean-Up System (Promega, WI). The final amplicon products were quantified using the Qubit dsDNA HS Assay kit (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... eloB and eloC) were purified on an agarose gel using the Wizard ® SV gel and PCR clean-up system purification kit (Promega). After restriction with the corresponding restriction enzymes (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... the bands of desired sizes were gel-purified using Promega Wizard® SV Gel and PCR Clean-Up System (Promega, USA). PCR fragments were assembled into the Universal Level 0 vector (pAGM9121 ...
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bioRxiv - Genetics 2024Quote: ... Resulting amplicons were purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega cat no. PR-A9281) then cloned into TOPO TA pcR2.1 (Invitrogen cat no ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Neuroscience 2023Quote: GFP- or Flag-synapsin constructs were separately transfected into HEK 293 cells using Lipofectamine 2000 (Promega). Transiently transfected cells were washed with PBS and lysed in immunoprecipitation buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged SPUD in vitro expression was done with Transcend Non-Radioactive Translation Detection System (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) and left to express for 24 hours ...
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
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bioRxiv - Genetics 2019Quote: ... DNA fragments were separated by agarose-gel electrophoresis and purified with the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA). Sequence authenticity was confirmed at the Massey Genome Centre ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was run on 2% agarose gel and the expected bands were eluted using Wizard® SV Gel and PCR cleanup kit (Promega, USA),cloned in pGEM-T easy vector (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... 10 spots were picked from the gel using an Ettan® SpotPicker™ and were in-gel digested with either trypsin (Promega), chymotrypsin (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... All PCR products were checked on 2% agarose gels and then cleaned with the Wizard SV Gel and PCR Clean-up System from Promega (Madison, Wisconsin) and sent off for Sanger-sequencing along with the amplification primers to Molecular Cloning Laboratories (San Francisco ...