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Citations for Promega :
1 - 50 of 1170 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1x ProTEV digestion buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TPMT-FLAG or TPMT A80P-FLAG with FuGENE 6 (Promega). 24 h post transfection ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells were transfected with FLAG-JAK1 or FLAG-JAK1Gly590Arg and HA-SOCS1 (FuGENE HD, Promega). 72 h later ...
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bioRxiv - Microbiology 2023Quote: ... and plasmids (pX-FLAG-hAGO2 and pX-FLAG-HrAGO1) were purified using PureYield Plasmid Miniprep System (Promega).
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
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bioRxiv - Biochemistry 2019Quote: ... These cells were then transfected with 50 ng of pcDNA3-STING-HA and 500 ng of either pcDNA3-FLAG-HA or pcDNA3-FLAG-HA-SLC19A1 using FuGENE 6 transfection reagent (Promega). After 24 h ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs were obtained from Origene in pCMV6 vector that also incorporates C-terminal Myc and FLAG tags (Myc-FLAG) or subcloned into a pHTC (Promega)-derived vector that expresses C-terminal HaloTag (41) ...
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bioRxiv - Immunology 2019Quote: MSR1 KO BMDMs were transfected either with 1 μg of pCMV WT-MSR1-FLAG or pCMV MSR1-(K27R)-FLAG using Fugene (Promega) transfection agent for 24 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... affinity-purified and acetone-precipitated Pex14p was proteolytically digested with trypsin (Promega), AspN (Sigma-Aldrich) ...
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bioRxiv - Genetics 2023Quote: ... SIRT5 WT and variants were purified by Ni2+-affinity resin (#V8823, Promega), and eluted with 25 mM Tris/HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... and pLenti RACK1-FLAG using Fugene HD (Promega). The lentivirus-containing media was filtered through a 0.45 μm PES filter ...
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bioRxiv - Immunology 2022Quote: The Flag-TRAF6 preparations eluted from FLAG resin with 3X FLAG peptide were digested with a combination of trypsin and Lys-C protease (Promega, #V5071) using S-trap mini columns (Protifi ...
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bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... or FLAG-KrascomQ61R using FuGene 6 reagent (Promega, E2691) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... gel-purified (Wizard SV Gel and PCR Clean-Up System, Promega) and blunted using PfuUltra HF DNA polymerase (Agilent Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... Then the gel was dried using a gel drying kit (Promega) and exposed to a phosphor screen (overnight) ...
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bioRxiv - Cell Biology 2024Quote: ... Gel pieces were destained and in-gel trypsin digested (Promega, V5280). The peptide mixture was purified on PepClean C18 spin columns (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... excised gel segments were subjected to in-gel trypsin (Promega, V5111) digestion and dried ...
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bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
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bioRxiv - Molecular Biology 2022Quote: ... Each lane was sliced into nine gel bands and the excised gel pieces were subjected to in-gel digestion using trypsin (Promega) followed by C18 STAGE tip clean-up (as described in 4).
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bioRxiv - Molecular Biology 2021Quote: ... Following gel extraction using Wizard SV Gel and PCR CleanUp System (Promega), 500 ng of purified and linearized vector and 200 ng of each specific gene were treated with T4 DNA polymerase enzyme (Fermentas ...
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bioRxiv - Evolutionary Biology 2021Quote: ... then gel extracted (Promega Wizard SV Gel and PCR Clean-Up System). Linear plasmids were subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... and gel slices were subjected to in-gel digestion with Trypsin (Promega) at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Individual gel piece was subjected to in-gel digestion using trypsin (Promega). The resulting dried peptides were analyzed in technical duplicate on a ThermoFisher Orbitrap Fusion Lumos Tribrid mass spectrometry system equipped with an EASY-nLC 1200 ultrahigh-pressure liquid chromatography system interfaced via a Nanospray Flex nanoelectrospray source ...
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bioRxiv - Microbiology 2023Quote: ... gel using the Wizzard SV Gel and PCR clean system (Promega, USA) and quantified with Qubit dsDNA HS assay (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2019Quote: ... and gel extraction (Wizard™ SV Gel and PCR Cleanup System [Promega A9282]) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were subjected to in-gel digestion with sequencing-grade trypsin (Promega), and the extracted peptides were resolved by reverse phase HPLC ...
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bioRxiv - Molecular Biology 2020Quote: ... pcDNA vectors (pcDNA-TruB1-Flag) were transfected with FuGENE HD (Promega) into cells according to the Manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... or Flag-eGFP-puro were transfected using Fugene HD (Promega E2311). On the following day ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified by agarose gel electrophoresis and extracted from the gel using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Purified PCR products were cloned into a pCR2.1-TOPO vector (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was run on a 2% agarose gel using gel electrophoresis and the band was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, USA). Samples were submitted to Macrogen Europe (Amsterdam ...
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bioRxiv - Microbiology 2021Quote: ... and gel bands excised before in-gel digestion using modified trypsin (Promega, sequencing grade). Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Cell Biology 2022Quote: Gel bands were subjected to in-gel digestion with sequencing-grade modified trypsin (Promega) as detailed in Belluzzi et al. ...
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bioRxiv - Genomics 2022Quote: ... products were gel purified using the Wizard SV Gel and PCR Cleanup kit (Promega) and ligated into pDONR221-b1b4 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were subsequently gel-extracted (Wizard SV Gel and PCR Clean-Up System, Promega), quantified ...
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bioRxiv - Cell Biology 2022Quote: ... The gel slices were reduced and in-gel digested with sequencing grade modified trypsin (Promega). The resulting samples were quenched by 10% trifluoroacetic acid and peptides were extracted and dissolved in 0.1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... gel purified using a Wizard® SV gel and PCR clean up system (Promega, Australia) and sequenced using an Illumina MiSeq platform at the Ramaciotti Centre for Genomics ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were gel-purified using Wizard® SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 4 Fluorometer (Thermofisher scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5-6ng pcDNA3.1 FLAG-cGAS and STING using 0.75μl FuGENE 6 (Promega) and 20μl Opti-MEM (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were concentrated and gel purified using Wizard SV Gel and PCR cleanup protocol (Promega). Binding reactions consisted of the manufacturer’s binding buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were gel-purified using the Wizard SV Gel and PCR Clean-Up System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Gel bands for heavy and light chains were excised and in-gel digested using trypsin (Promega), chymotrypsin (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR fragment was gel-purified using Wizard SV gel and PCR Clean-up System (Promega), ligated into pGEM-T (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... DNA fragments were excised from the gel by means of x-tracta™ Gel Extractor (Promega) and purified using Wizard® SV Gel and PCR Clean-Up System (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... in-gel digestion with trypsin (Promega) overnight ...
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bioRxiv - Immunology 2022Quote: Human LRBA was cloned in seven different fragments into pCIneo FLAG vectors (Promega). These plasmids were kindly provided by Dr ...
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bioRxiv - Cell Biology 2023Quote: ... and pMRXIH together with 3×FLAG tag or HaloTag7 (N2701, Promega, Madison, WI). DNA fragments encoding ATG2A (NM_015104.3 ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR products of the Strep-Flag-tag and NLuc (Promega, cat # N1091) were inserted into the pHLmMBP-8 vector (Addgene ...