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Citations for Promega :
251 - 300 of 1170 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: For in vitro pulldown experiments the coding region of AtMED25Δq was fused N-terminally with a flag-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega). RAP2.12 C-terminally fused to CFP was previously established (Schmidt et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were in-gel digested with trypsin (Promega, Madison, WI, USA), and the resulting peptide mixtures were processed on StageTips (Rappsilber ...
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bioRxiv - Genetics 2019Quote: ... after reducing SDS-PAGE on a gradient (4-15%) polyacrylamide gel (Promega) and transfer onto nitrocellulose membrane ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were in gel-digested overnight with sequencing grade trypsin (Promega). The resulting tryptic peptides were desalted using in-house C18 disks ...
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bioRxiv - Biochemistry 2020Quote: ... Each reaction was then gel purified using the Wizard SV kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: GST-AIMP1 was in-gel digested with Trypsin GOLD (Promega, Madison, WI). Tryptic peptides were separated by reversed-phase chromatography using Eazynano LC II (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... and proteins therein were in-gel digested with trypsin (Promega, Dübendorf, Switzerland), and resulting peptide mixtures were processed on STAGE tips and analyzed by LC-MS/MS.
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bioRxiv - Biochemistry 2020Quote: ... followed by in-gel digestion over night with 50 ng trypsin (Promega) in 50 mM TEAB ...
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bioRxiv - Microbiology 2021Quote: ... using The Wizard® SV Gel and PCR Clean-Up Kit (Promega), and ligated to pEVS143 via Gibson Assembly ...
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bioRxiv - Bioengineering 2021Quote: ... Those were purified by wizard gel and PCR clean-up kit (Promega), achieving a final concentration of 200 ng/µL ...
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bioRxiv - Genomics 2022Quote: ... purified by Wizard® SV Gel and PCR Clean-Up System (Promega), and labelled using nick translation protocol by (Kato et al. ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR fragments were cleaned using a gel extraction kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and cloned into the vector pCR2.1 using the TOPO TA Cloning Kit (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... in-gel digestion was performed using 0.10 µg trypsin/Lys-C (Promega) overnight in 25 mM NH4HCO3 at 30 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described71 ...
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bioRxiv - Genomics 2023Quote: ... PCR products were purified via gel excision and column purification (Promega, A9282), followed by insertion into the BsmBI–digested lentiV2-hU6-evopreQ1 vector by Gibson assembly (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then in-gel digested using modified trypsin (sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested in-gel using trypsin (600 ng/band, Gold, Promega), as previously described [29] ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were cleaned using a gel extraction kit (Promega) according to the manufacturer’s protocol and used for cloning using a Gibson assembly protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... both using the Wizard SV Gel and PCR Clean-up System (Promega). Inserts were incorporated into the vector via ligation reaction consisting of inserts and vector in a 5:1 molar ratio ...
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bioRxiv - Biochemistry 2023Quote: ... proteins isolated by gel electrophoresis were digested with trypsin (Promega, Wisconsin, USA) in 100 mM NH4HCO3 (pH 8.0) ...
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bioRxiv - Neuroscience 2024Quote: ... Anesthetized zebrafish were embedded in low melting point agarose gel (Promega, V2111) in a confocal dish ...
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bioRxiv - Cell Biology 2023Quote: ... the gel pieces were incubated with 125 ng trypsin (sequencing grade; Promega) in 50mM NaHCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... the gel pieces were incubated with 125 ng trypsin (sequencing grade; Promega) in 50mM NH4HCO3 ...
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bioRxiv - Genetics 2023Quote: ... PCR products were gel-purified and cloned into pGEM-T Easy (Promega) for Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... images produced from HeLa cells seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) and transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) for 24 hours ...
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bioRxiv - Genetics 2021Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega), cloned into the pGEM-T Easy vector (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) or MinElute® PCR Purification Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaned with Wizard SV Gel and PCR Clean-Up system (Promega, Madison, WI) and cloned into One Shot TOP10 cells with TOPO-TA cloning kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragments excised from the gel purified by the DNA Purification kit (Promega) according to the maker instructions.
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bioRxiv - Molecular Biology 2022Quote: ... and the excised gel slices were incubated with sequencing-grade modified trypsin (Promega) to extract the peptides ...
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bioRxiv - Bioengineering 2022Quote: ... and subjected to in-gel digestion with 1.25 μg trypsin (PROMEGA, sequential grade) or 1 μg Asp-N (NEB ...
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bioRxiv - Developmental Biology 2022Quote: ... and purified using a Wizard SV Gel and PCR Clean-up System (Promega). mRNA was generated from linearized plasmid using an SP6 mMessage mMachine Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... In-gel digestion was performed overnight by using trypsin/LysC (Promega, Madison, WI). Peptides extracted from each band were analyzed by nanoLC-MS/MS using an Ultimate 3000 system (Dionex ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were in gel digested with trypsin (Trypsin Gold, mass spectrometry grade, Promega) overnight at 37 °C ...
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bioRxiv - Genetics 2022Quote: ... the gel extracted product was ligated into the pGEM®-T Vector (Promega) using the supplied kit per manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... The Wizard SV Gel and PCR Clean-Up System were purchased from Promega while the GeneJet Plasmid Midiprep Kit was purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Synthetic Biology 2020Quote: ... Amplicons from the second PCR were then pooled and gel extracted (Promega, A9282) to make the final library ...
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bioRxiv - Microbiology 2020Quote: ... pKAS32 was digested with XbaI and SacI and purified by gel extraction (Promega). Primers (Integrated DNA Technologies ...
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bioRxiv - Zoology 2021Quote: ... PCR products were purified with Wizard SV Gel and PCR Cleanup kit (Promega). Purified products were sequenced at Sequetech DNA Sequence Service (Mountain View ...
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bioRxiv - Cell Biology 2019Quote: ... gel pieces were dried and rehydrated with 12.5ng/mL trypsin (Promega, Madison, WI) solution in 25mM ammonium bicarbonate on ice for 30min ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were purified via gel excision and column purification (Promega, A9282), and then inserted into the BsmBI-digested lentiCRISPR-v2-Blast-Puro vector by Gibson assembly (New England Biolabs ...
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bioRxiv - Physiology 2020Quote: ... PCR amplicons were resolved on a 2% agarose gel (Promega Corporation, Madison, USA) pre-stained with Ethidium bromide (0.5 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was purified by gel extraction and digested with DpnI (Promega) for 1 h to remove residual template DNA before a second round of purification ...
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bioRxiv - Cell Biology 2019Quote: ... Gel slices were rehydrated with a solution of 12.5 ng/µl trypsin (Promega Trypsin Gold ...
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bioRxiv - Cell Biology 2022Quote: Proteins were digested from gels with 0.02 μg/μL modified porcine trypsin (Promega) after reduction with dithioerythritol and iodoacetamide ...
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bioRxiv - Molecular Biology 2022Quote: Purified EXT1-EXT2 was digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described67 ...
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bioRxiv - Plant Biology 2022Quote: ... The NPR1-YFP band was cut and in-gel digested by trypsin (Promega). The phosphorylated peptides of NPR1 protein were detected by LC-MS/MS at the Mass Spectrometry facility at CAS for Excellence in Molecular Plant Sciences.
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bioRxiv - Immunology 2022Quote: ... and purified with the Wizard SV Gel and PCR Clean-Up System (Promega) after DNA electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: The protein in the gel lanes were digested with 0.1 µg trypsin (Promega) in 20 µl 25 mM ammonium bicarbonate ...