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Citations for Promega :
1551 - 1600 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Two aliquots of 100 µL were transferred into new tubes and digested either with a sequencing-grade trypsin (Promega, Madison, WI, USA) or chymotrypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then digested overnight at 35ºC using Trypsin/Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega, Cat No: V5072); the enzyme-substrate ratio of 1:70) ...
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bioRxiv - Cell Biology 2023Quote: ... Reduced and alkylated samples were digested in-gel using 200 μL (or a sufficient volume to cover all the gel pieces) of MS grade trypsin (Promega – Cat.#V5111), diluted to 10 ng/µL in 40 mM TEABC buffer in 1.5-mL proteomics-compatible tubes ...
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bioRxiv - Biochemistry 2023Quote: ... All samples were then diluted 8-fold with 100 mM NH4HCO3 and 1 mM CaCl2 and digested with sequencing-grade modified porcine trypsin (Promega, WI, USA) provided at a 1 ...
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bioRxiv - Genomics 2022Quote: ... The samples were diluted 1:1 with milliQ water and were incubated with a 1:100 w/w amount of trypsin (Promega, sequencing grade) overnight at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Urea concentration was further adjusted to 2M using 50mM Tris pH 8 and Lysyl peptides were additionally digested with sequencing grade trypsin (Promega, Madison, WI) in an enzyme/substrate ratio of 1:20 at 37°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... diluted by the addition of 7 volumes of 25 mM Tris-HCl pH 8.0 and sequencing-grade modified Trypsin (Promega Corp., Madison, WI) was added (0.4 μg/ sample ...
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bioRxiv - Systems Biology 2023Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Biophysics 2023Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 ug in 10 uL of 50 mM TEAB; Promega, Madison, WI). Following this an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2023Quote: ... The captured proteins were washed twice in 200 μl of 95% MeCN and transferred to 200 μl of 50 mM ammonium bicarbonate (ABC) containing 1 μg sequencing grade modified trypsin (Promega, Madison, USA) and mixed for 2 hr at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was performed in the S-Trap overnight at 37°C using Trypsin/Lys-C Mix Mass Spec Grade (Promega, Walldorf, Germany), followed by elution in 50 mM TEAB ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted by the addition of 7 volumes of 25 mM Tris-HCl pH 8.0 and sequencing-grade modified Trypsin (Promega Corp., Madison, WI) was added (0.4 μg/ sample ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were then dried and resuspended in 50 mM ammonium bicarbonate and incubated with sequencing-grade modified trypsin (Promega, Cat. No. V5113) in a 1:20 enzyme:sample ratio overnight at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were diluted by the addition of 7 volumes of 25mM Tris-HCl pH 8.0 and sequencing-grade modified Trypsin (Promega Corp., Madison, WI) was added (0.4 μg/ sample ...
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Ethanolamine-induced assembly of microcompartments is required for Fusobacterium nucleatum virulencebioRxiv - Microbiology 2024Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 µg in 10 µl of 50 mM TEAB; Promega, Madison, WI) following which an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2024Quote: One hundred micrograms of urinary proteins from each sample were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of proteins from the lysates were digested following the SP3 protocol [83] with Sera-Mag™ carboxylate-modified SpeedBeads [E7] and [E3] (Cytiva 45152105050250 and 65152105050250) and sequencing grade modified trypsin (Promega V5113). The digested peptides were acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Developmental Biology 2024Quote: ... then both soluble and insoluble protein lysates were reduced with 5 mM dithiothreitol and further alkylated with 15 mM iodoacetamide and processed for overnight digestion at 37°C in S-Trap columns (Protifi) using a Sequencing Grade Modified Trypsin (Promega, Catalog # V5111). The peptide mixtures were eluted from the columns ...
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bioRxiv - Microbiology 2020Quote: ... with 600 μL of Nuclei Lysis Solution (Promega, Wisconsin) and incubated at 65 C for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... 20 μL of Cell Titer Aqueous One Solution (Promega) was added to the wells and after incubation of 2 hours at 37°C the absorption at 492 nm was measured.
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bioRxiv - Cancer Biology 2020Quote: ... CellTiter 96 AQueous One Solution (Promega, Walldorf, United States) was used to determine relative cell proliferation every other day ...
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bioRxiv - Cell Biology 2021Quote: ... 20μl of CellTiter 96 Aqueous One Solution (G3582, Promega) was dispensed per well ...
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bioRxiv - Synthetic Biology 2021Quote: ... a 100mM stock solution of beetle luciferin (Promega; E1602) was prepared in 10mM HEPES and stored at -20°C protected from light ...
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bioRxiv - Synthetic Biology 2021Quote: ... a 100X stock solution of cAMP reagent (Promega; E1290) was prepared in 10mM HEPES and stored at -80°C ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl solubilization Solution/Stop Mix (Promega cat. #G4101) was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... or CellTiter-Glo® One Solution Assay system (Promega) following manufacturer protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... 1X GoTaq Green Master Mix solution (Promega, Wisconsin, USA) and nuclease-free water (Promega ...
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bioRxiv - Immunology 2021Quote: ... then 30 μl of Luciferase Assay System solution (Promega) added and luminescence read immediately using a Cytation5 plate reader.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the Cell Titer Glo One solution kit from Promega was used ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the Cell Titer Glo One solution kit from Promega was used ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the Cell Titer Glo One solution kit from Promega was used ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CellTiter-Glo 2.0 Cell Viability Assay solution (Promega, USA) was added ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then transfected in solution using Fugene6 (Promega) according to a modified version of the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... or the lytic substrate (HiBiT lytic working solution, Promega), for the total luminescence samples.
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bioRxiv - Cell Biology 2024Quote: ... a solution containing 50 nM JF549-HTL (Promega GA1110) was applied for 15 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 10 μL of CellTiter 96 AQueous One solution (Promega) was added to each well ...
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bioRxiv - Bioengineering 2023Quote: ... CellTiter-Glo® 2.0 Cell Viability Assay solution (Promega) was added to 384-well plates ...
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bioRxiv - Bioengineering 2023Quote: ... CellTiter-Glo® 2.0 Cell Viability Assay solution (Promega) was added directly to the FLECSplates for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... 40 µL of luciferase assay substrate solution (Promega E1501) was added and luminescence was measured using a plate reader (Tecan Infinite®200 Pro) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50uL of 3x complete substrate plus inhibitor solution (Promega) was added to each well and read after 2 min on a GloMax instrument ...
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bioRxiv - Biochemistry 2023Quote: 20 µl of CellTiter-Glo (CTG) luminescent solution (Promega) was added in each well after equilibration of the 96 well plate and CTG solution at room temperature for 20 min ...
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bioRxiv - Bioengineering 2023Quote: ... CellTiter 96 AQueous One Solution (Promega, Madison, WI, USA) were used ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with trypsin (Promega) overnight at room temperature ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... CellTiter 96® AQueous One Solution Reagent (Promega, G3580), at one-fifth of the culture volume was added to the wells and incubated for 2 hrs ...
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bioRxiv - Cancer Biology 2024Quote: ... 6X Cell Titer Blue solution (Promega Corporation, Madison, WI) was added into wells with cells and wells with plain media as a no cell control ...
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bioRxiv - Microbiology 2024Quote: ... and the protein solution was digested with trypsin (Promega) overnight at room temperature ...