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Citations for Promega :
1401 - 1450 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated for 30 minutes in the dark with 15mM iodoacetamide and then digested with MS-Grade Modified Trypsin according to the Trypsin Digestion Protocol (Promega). Digested Samples were phospho-enriched using the High-Select Fe-NTA Phosphopeptide Enrichment Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by a digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... then resuspended in 1 gel volume of 25 mM ABC containing 5 ng/µL of digestion grade trypsin (Promega, V5111) and incubated at 37°C for 16 hours for a full digestion or for 15 minutes for a partial digestion ...
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bioRxiv - Biochemistry 2024Quote: ... and then digested overnight at 37 °C and shaking at 1000 rpm (TC) with trypsin (Sequencing Grade Modified Trypsin V5113, Promega) and LysC (Lysyl Endopeptidase 129-02541 ...
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bioRxiv - Systems Biology 2024Quote: ... followed by three washes with 100% acetonitrile before incubation with digestion buffer (50 mM TEAB, containing Sequencing grade Trypsin (Promega) at 1:25 enzyme-to-protein for 4 hours ...
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bioRxiv - Systems Biology 2024Quote: ... On-bead protein digestion was performed overnight in 50 mM triethylammonium bicarbonate (TEAB) pH 9 with mass spectrometry-grade trypsin (Promega) and LysC (FUJIFILM ...
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bioRxiv - Biophysics 2024Quote: ... This was proceeded by overnight digestion at 37 degrees Celsius with 500ng of mass spectrometry grade trypsin/LysC mix (Promega). Post-digestion ...
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bioRxiv - Cell Biology 2024Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (0.5 µg protease, Mass Spectrometry grade, Promega V5072). Peptide Purification and Labeling ...
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bioRxiv - Biochemistry 2024Quote: ... S-trap columns containing the bound sample proteins were digested with sequencing grade modified trypsin (Promega, Fitchburg, WI, Cat # V5111). Upon completion of digestion ...
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bioRxiv - Cell Biology 2024Quote: After the final wash the beads were transferred to a new tube and resuspended in 30 µL 2 M urea in 50 mM ammonium bicarbonate and digested with 75 ng LysC (mass spectrometry grade, FUJIFILM Wako chemicals) and 75 ng trypsin (Trypsin Gold, Promega) at room temperature for 90 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were digested at room temperature overnight using 1:100 (m/m) sequencing grade trypsin (cat. no. V5111, Promega, USA).
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bioRxiv - Developmental Biology 2024Quote: ... Samples were prepared using the SP3 protocol (Hughes, et al. 2014; Hughes, et al. 2019) and trypsin (sequencing grade, Promega) was added in an enzyme to protein ratio of 1:50 for overnight digestion at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... cellular ATP concentrations were measured by adding 20 μL of 1x CellTiterGlo 2.0 (CTG) solution (1 part PBS, 1 part CellTiterGlo2.0 stock solution; Promega Cat# G9241) using a MultidropTM Combi liquid dispenser and measuring the luminescence on a SpectraMax iD3 (Molecular Devices).
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bioRxiv - Genetics 2021Quote: ... PCR products were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-90 real-time PCR System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using Wizard SV Gel and PCR Clean-Up System (Promega) according to the instructions by the manufacturer ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were cleaned with the Wizard SV Gel and PCR Clean Up Kit (Promega) and cut with XhoI and BamHI or HindIII und BamHI (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified by the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified using the Qubit dsDNA HS assay (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified with Wizard® SV Gel and PCR Clean-Up System (Promega) and ligated to the pGEM-T easy® vector (Promega) ...
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bioRxiv - Pathology 2021Quote: ... Quantitative RT-PCR was performed by using the Go-Taq Real-Time PCR system (Promega), as previously described60 ...
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bioRxiv - Bioengineering 2021Quote: ... Input linear DNA was produced by PCR and cleaned with PCR cleanup kit (Promega, A6754), quantified and diluted to desired concentrations using ultra-pure water ...
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bioRxiv - Immunology 2024Quote: The PCR products representing genomic and cDNA sequences were purified using PCR purification kit (Promega) and subsequently sequenced using Sanger sequencing at Micromon Genomics ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative real-time PCR (RT-qPCR) was performed using GoTaq PCR Master Mix (Promega, #A6002) and specific primers listed below ...
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bioRxiv - Immunology 2023Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed with the GoTaq qPCR Mastermix (Promega) using the CFX-90 real-time PCR System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified with Wizard SV Gel and PCR Clean-Up System (Promega). A volume of 0.75 µl of the DNA templates was mixed in 2.5 µl cell-free protein expression reactions (PUREFrex 2.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR cleanup was conducted using the Wizard SV Gel and PCR Clean-Up System (Promega) with elution in 30 μl of double distilled water ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) in line with the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: Sanger sequencing of PCR products (purified with Wizard SV Gel and PCR Cleanup System (Promega)) or plasmids (purified with GenELuteTM Plasmid Miniprep Kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified using Wizard® SV Gel and PCR Clean-Up System (Promega) and sequenced using Sanger method (44).
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bioRxiv - Microbiology 2020Quote: ... 200 μL of Protein Precipitation Solution (Promega, Wisconsin) was added ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μl per well of MTS solution (Promega) was added for two hours and then absorbance was recorded at 490nm with a 96-well plate reader ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL One-Glo luciferase substrate solution (Promega) was added to each well prior to reading the signal on the Envision plate reader (Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... 75 μL of Nano luciferase substrate solution (Promega) was added to each well ...
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bioRxiv - Biophysics 2022Quote: In-solution digestion protocol was adapted from Promega protocols for Trypsin Gold and Chymotrypsin (Promega) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were treated with RNase (RNaseA Solution, Promega) for 30 min and proteins were denatured for 10 min at 90 °C in 2× SDS loading buffer with 10 mM DTT ...
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bioRxiv - Cancer Biology 2022Quote: ... CellTiter 96 AQueous One Solution (MTS reagent; Promega) was added and the plates incubated for a further 3 hours at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Furimazine (Fz) stock solution was purchased from Promega as a component of the Nano-Glo® Luciferase Assay System (cat# N1110 or N1120) ...
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bioRxiv - Systems Biology 2023Quote: ... 10 µl of MTase-Glo Detection Solution (Promega) was added ...
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bioRxiv - Cell Biology 2023Quote: ... add 600 μl of Nuclei Lysis Solution (Promega) and mix by pipetting ...
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bioRxiv - Molecular Biology 2023Quote: ... then 25 μl MTa-Glo Detection Solution (Promega) was added and incubated for a further 30 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Add 200 μl of Protein Precipitation Solution (Promega) and mix by pipetting ...
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bioRxiv - Microbiology 2024Quote: ... and 50 µl NanoLuc working solution (NanoGlo, Promega) was added to the wells ...
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bioRxiv - Physiology 2024Quote: ... Samples were in-solution digested with trypsin (Promega). Tryptic peptides were purified by STAGE tips and LC-MS/MS measurements were performed on a QExactive plus and an Orbitrap Exploris 480 mass spectrometer coupled to EasyLC 1200 nanoflow-HPLCs (all Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein solutions were digested using trypsin (Promega, V5280) at a ratio of 1:150 (w/w ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 40 mM ammonium bicarbonate and digested by 1 ug sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight ...
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bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were diluted 10-fold with 100 mM Tris (pH 8.0) and incubated overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1h ...
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bioRxiv - Plant Biology 2020Quote: ... The dried gels were incubated for 16 h at 37°C in 50 mM ammonium bicarbonate containing sequence grade modified trypsin (Trypsin Gold; Promega, USA). The digested peptides were eluted from the gels with 50% acetonitrile (v/v ...