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Citations for Promega :
1651 - 1700 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:50 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072).
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bioRxiv - Cell Biology 2024Quote: ... in-gel digestion was performed on 30 µL of the provided secretomes using trypsin (300 ng sequencing grade modified trypsin V5111; Promega, Leiden, The Netherlands) after first reducing them with 10 mmol/L dithiothreitol and then alkylating with 55 mmol/L iodoacetamide proteins [21] ...
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bioRxiv - Developmental Biology 2024Quote: ... S-trap columns containing the bound sample proteins were transferred to 1.5 ml Lobind centrifuge tubes and 40 µl of 80 ng/µl sequencing grade modified trypsin (Promega, Fitchburg, WI, Cat # V5111) in 50 mM TEAB was added ...
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bioRxiv - Genomics 2020Quote: ... or PCR master mix (Promega, Wisconsin, USA). For ExTaq polymerase reactions ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed using GoTaq Green (Promega) according to the vendor protocol ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed using GoTaq Green (Promega) according to the vendor protocol ...
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bioRxiv - Microbiology 2022Quote: PCR clean-up system (Promega, catalog #: A9285).
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bioRxiv - Microbiology 2020Quote: ... Transformants were screened using GoTaq PCR (Promega) with universal M13F (−20 ...
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bioRxiv - Biophysics 2021Quote: ... using the Access RT-PCR System (Promega), and then exchanged with exon 6b in mH1 using the Quikchange method of site-directed mutagenesis (SDM ...
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bioRxiv - Microbiology 2022Quote: Colony PCR using GoTaq DNA polymerase (Promega) was performed on isolates not identified by MALDI-TOF MS ...
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bioRxiv - Microbiology 2023Quote: PCR products and a standard ladder (Promega) were electrophoresed on 1% agarose gels in 1X TBE for 1 hr at 85V ...
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bioRxiv - Molecular Biology 2023Quote: ... a PCR using GoTaq G2 polymerase (Promega) was performed to specifically amplify the wild-type target locus ...
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bioRxiv - Neuroscience 2023Quote: ... then PCR amplified with GoTaq MasterMix (Promega) and subsequently digested with a restriction enzyme to distinguish wild type and mutant alleles (Table 1) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed with GoTaq Flexi (Promega) for 30 cycles of ...
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bioRxiv - Biochemistry 2024Quote: ... 4 µL Mg2+ free PCR buffer (Promega), 4 µL 25 mM MgCl2 ...
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bioRxiv - Plant Biology 2024Quote: ... using GoTaq Long PCR Master Mix (Promega). Construct development was performed as described in Bettgenhaeuser et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by PCR amplification with GoTaq (Promega). The primers are listed in Table 1.
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bioRxiv - Neuroscience 2024Quote: ... Following PCR with GoTaq polymerase (Promega M7123) and primers (Table S1) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCRs were performed using GoTaq G2 (Promega), Q5 (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR product was purified by Promega PCR clean-up system and cloned into a pGEM-T Easy Vector for sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... CellTiter 96® Aqueous One Solution Cell Proliferation Assays (Promega) were performed according to manufacturer specifications ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was transferred to a 200 μl column (Promega). Unbound proteins were collected by centrifugation at 800 ×g for 1 min ...
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bioRxiv - Neuroscience 2020Quote: ... Pellet was resuspended in RQ1 DNase solution (Promega, Madison, WI) and incubated for 1 hr at 37° with continuous agitation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 20 μL of CellTiter 96® AQueous One solution (Promega) was added to cells in a 96-well plate containing 100 μL culture medium ...
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bioRxiv - Microbiology 2021Quote: ... and 25 μL of diluted Nano-Glo live solution (Promega) was added into the each well ...
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bioRxiv - Cancer Biology 2020Quote: ... 40μL of the CellTiter 96® AQueous One Solution (Promega) was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... CellTiter 96® AQueous One Solution Assay (Promega, Southampton, UK); Human CD34+ Progenitor Cells (hCD34+-CB ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Halys using RNAgent® Denaturing Solution (Promega, Madison, Wisconsin, USA), quantified in a micro-volume spectrophotometer Biospec-Nano (Shimadzu ...
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bioRxiv - Molecular Biology 2020Quote: ... using CellTiter 96 AQueous one solution cell proliferation assay (Promega) following manufacturer instructions ...
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bioRxiv - Biochemistry 2021Quote: ... A 0.1 µg/µl stock solution of trypsin (Promega, V511A) in trypsin resuspension buffer (Promega ...
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bioRxiv - Microbiology 2021Quote: ... After stopping the reaction with RQ1 DNase Stop Solution (Promega) for 10 minutes at 65°C ...
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bioRxiv - Neuroscience 2023Quote: ... In-gel digestion with Trypsin/Lys-C Mix solution (Promega) was performed as previously described (Gonzalez-Lozano & Koopmans ...
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bioRxiv - Cell Biology 2024Quote: ... a labeling solution containing 100 nM JF646-HTL (Promega GA1121) in phenol red-free culture medium was freshly prepared ...
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bioRxiv - Microbiology 2023Quote: ... bovine serum albumin solution (1 μg/μL) (Promega, United States), Lucigen Ready-Lyse lysozyme (500 U/μL ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% v/v of RQ1 RNase/DNase Stop Solution (Promega) and 10% v/v Oligo(dT ...
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bioRxiv - Developmental Biology 2024Quote: ... and digested according to the In-solution Protein Digestion (Promega) protocol ...
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bioRxiv - Cancer Biology 2020Quote: HPV16 integration into the IRF4 gene was confirmed by PCR using GoTaq Long PCR Master Mix kit (Promega U.S.), with 5’-AAGCATGTCAGACACGCAGA- 3’as a forward primer ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... PCR products were gel purified with either Wizard® SV Gel and PCR Clean-Up System (Promega cat# A9281) or Isolate II PCR and Gel Kit (Bioline cat# BIO-52059 ...
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bioRxiv - Biochemistry 2020Quote: ... PCR reaction mixture was purified with the Wizard® SV Gel PCR Clean-Up System (Promega Corporation, WI, USA). One Shot® TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
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bioRxiv - Genetics 2020Quote: ... Founder mice were analyzed by PCR using primers described in Table S1 and PCR products were subcloned in pGEM.T Easy Vector for sequencing (Promega). F1 and F2 mice were confirmed by PCR and by PCR products sequencing (primers available in Table S1) ...
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bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the WizardTM SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) following the manufactures instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control ...
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bioRxiv - Biochemistry 2023Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were gel-extracted using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison WI, USA) and sequenced by GENEWIZ (Leipzig ...
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bioRxiv - Bioengineering 2024Quote: ... The obtained PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (A928, Promega) and blunt end ligation was performed followed by electroporation of freshly made competent cells [36] to yield 3.6×107 total transformants ...
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bioRxiv - Physiology 2024Quote: ... The PCR products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, #A9281). To prepare the blunt-ended PCR products for cloning ...
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bioRxiv - Biochemistry 2020Quote: ... The gel piece was vacuum dried and treated with trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche sequencing grade) at 37°C for 16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were diluted 10-fold with 50 mM triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) at a 1:50 enzyme:substrate ratio (wt/wt) ...