Labshake search
Citations for Promega :
1701 - 1750 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... and pellets were washed twice with ice-cold ethanol followed by overnight incubation at 37°C in 100 mM ABC containing 1 mM CaCl2 and trypsin (Sequencing grade, modified, Promega Co, Madison, WI, USA). Digested proteins were evaporated and each sample was freed from salts and buffers by solid-phase extraction on C18 resin using cartridges prepared in-house ...
-
bioRxiv - Cell Biology 2023Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (40 µg of protein used for global proteomics and the remainder for phosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA ...
-
bioRxiv - Cancer Biology 2024Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1 µg protease used, Mass Spectrometry grade, Promega Corporation, Cat No: V5072). After acidification ...
-
bioRxiv - Plant Biology 2020Quote: ... The positive PCR products were cleaned using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI) and submitted for Sanger sequencing at Roy J ...
-
bioRxiv - Genetics 2022Quote: Chromatin accessibility by real-time PCR (ChART-PCR) was performed as previously described (75) using 20 U DNase I (Promega). To assess nucleosome occupancy ...
-
bioRxiv - Developmental Biology 2021Quote: ... The resulting cDNA was subjected to PCR or qPCR analysis using Phire Green Hot Start II PCR Master Mix (Promega) or GoTaq qPCR Master Mix (Promega) ...
-
bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Wisconsin, USA, A9281) and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes ...
-
bioRxiv - Biochemistry 2021Quote: ... Both PCR products were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation [37] ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR product was amplified with Phusion HotStart II DNA polymerase and the PCR fragment was ligated with T4 ligase (Promega) and transformed into Novablue competent cells ...
-
bioRxiv - Plant Biology 2020Quote: ... The overlapping PCR products were separated and purified by the Spin Column PCR Product Purification Kit (Wizard SV Gel and PCR Clean-Up System) following manufacturer’s instruction (Promega, WI). Then ...
-
bioRxiv - Microbiology 2021Quote: ... PCR amplicons were gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega Cat. No. A9281). Next ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA), quantified ...
-
bioRxiv - Cell Biology 2021Quote: ... DNA fragments containing the T7 promoter sequence were further amplified by PCR and purified using a Wizard SV Gel and PCR clean-up system (#A9281, Promega) to prepare templates for in vitro transcription ...
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed according to the method of Ehira and Miyazaki43 using Go Taq qPCR Master Mix (Promega). Primers used for PCR and qRT-PCR are listed in Supplementary Table 5.
-
bioRxiv - Plant Biology 2023Quote: ... PCR reactions were conducted in 10 μL volume comprising 5 μL Promega 2x PCR Master Mix (Promega, Madison, Wisconsin, USA), 10 pmol of forward and reverse primers ...
-
bioRxiv - Cancer Biology 2023Quote: ... The isolated RNAs (20 ng) were reverse-transcribed and quantitative PCR (qPCR) were perform using GoTaq Probe 1-Step RT-PCR system (Promega) in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
-
bioRxiv - Synthetic Biology 2023Quote: ... while the purification of the PCR fragments was performed using the Wizard SV Gel and PCR Clean-Up System (Promega).
-
bioRxiv - Plant Biology 2024Quote: ... PCR amplification of WLV was done using PuReTag Ready-to-Go PCR beads (Illustra) or Go Taq DNA polymerase (Promega) according to the manufacturers’ instructions and WLV-specific primer pairs 4194+4195 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Strings and plasmid backbones were amplified by PCR followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega). Plasmids were assembled using isothermal GibsonAssembly® reaction (NEBuilder® HiFi DNA Assembly Master Mix).
-
bioRxiv - Molecular Biology 2024Quote: ... and the amplified dsDNA during the PCR reaction was purified with Wizard SV Gel and a PCR Clean-Up System (Promega). Subsequently ...
-
bioRxiv - Microbiology 2024Quote: ... and the coding region of INKV L amplified by PCR and purified using the Wizard SV Gel and PCR Clean-up System (Promega). The INKV L fragment and the pINKV-L_linker backbone were digested with Aat II and Bsr GI (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2024Quote: Total cellular DNA was examined by quantitative PCR optimized for detection of ∼8-12kb fragments as previously described (33) using GoTaq Long PCR polymerase (Promega), Evagreen (1:20 Biotium) ...
-
bioRxiv - Genetics 2024Quote: ... Tissue and stage-specific cDNAs were used as templates to perform PCR using gene-specific primers (Table S2) and GoTaq PCR master mix (Promega). All the PCR reactions were performed in Proflex 32 thermocycler (Applied Biosystems ...
-
bioRxiv - Immunology 2021Quote: ... 400 µL PCR products were purified by Promega Wizard Gel/PCR purification columns ...
-
bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel extracted (Promega, A9281). Wildtype embryos were injected with a mix containing two sgRNAs (56.25 ng/μl each) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 mM PCR dNTP mix (Promega) and 0.5 μl RNase free water ...
-
bioRxiv - Zoology 2021Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
-
bioRxiv - Cell Biology 2021Quote: ... a PCR was done using Taq polymerase (Promega) and visualize on agarose gel to assess xbp1 ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR was performed using Go Green Taq (Promega) according to the vendor protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated to pGEM-T (Promega) or pBluescript SKII (− ...
-
Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Colony PCR was performed using GoTaq polymerase (Promega). The ΔhilE hilD::mCherry was constructed by incorporating hilD::mCherry construct into the ΔhilE strain.
-
bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
-
bioRxiv - Microbiology 2022Quote: ... Diagnostic PCR was performed using GoTaq polymerase (Promega) or OneTaq DNA polymerase.
-
bioRxiv - Cancer Biology 2022Quote: ... PCR was performed in GoTaq Master Mix (Promega) according to standard procedures ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR was performed with GoTaq DNA polymerase (Promega); primer sequences are shown in supplemental Table S1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
-
bioRxiv - Zoology 2020Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 ul MgCl ...
-
bioRxiv - Zoology 2020Quote: ... 10 μL 5× PCR buffer (Gotaq flexi, Promega), 8 μL 25mM MgCl ...
-
bioRxiv - Microbiology 2020Quote: ... PCR was performed with GoTaq Master Mix (Promega) with 1ul cDNA template at 1:20 dilution ...
-
bioRxiv - Bioengineering 2021Quote: ... and cleaned again using PCR cleanup (Promega, A6754). Plasmid DNA inputs were used in a set of concentrations ranging from 0.5 nM to 10 nM ...
-
bioRxiv - Molecular Biology 2022Quote: ... with mutagenesis performed by PCR using PfU (Promega). All mutagenesis was confirmed by Sanger Sequencing (Source Bioscience).
-
bioRxiv - Synthetic Biology 2022Quote: ... and 300 μM of PCR Nucleotide mix (Promega). The liposome suspensions were incubated at 30 °C in a thermocycler for the indicated time periods.
-
bioRxiv - Plant Biology 2022Quote: ... PCR was performed using GoTaq DNA Polymerase (Promega) with a melting temperature of 55 °C for SpCas9 ...
-
bioRxiv - Neuroscience 2022Quote: ... PCR was conducted using GoTaq DNA polymerase (Promega). Primer sequences were as follows ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR fragments were subcloned into pGEM-Teasy (Promega) and then moved as HindIII/NotI fragments into pCB4699 ...
-
bioRxiv - Physiology 2022Quote: ... qRT-PCR was performed using SYBR Green (Promega). The primers used were (5’-3’ ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCRs were performed using GoTag DNA polymerase (Promega) and primers annealing either of the flanking exons to look at the pattern of splicing or exon-junction (EJ ...
-
bioRxiv - Neuroscience 2024Quote: ... GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR ...
-
bioRxiv - Zoology 2024Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...