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Citations for Promega :
1551 - 1600 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Transfections were performed with Fugene 6 (Promega) per manufacturer’s directions for 24-48 hours before selection with 400 μg/mL hygromycin B.
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Biochemistry 2024Quote: ... after addition of the diluted NanoLuc substrate (10 μl/well, 30-fold dilution of the Promega substrate stock using the activation solution) ...
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bioRxiv - Biochemistry 2024Quote: ... containing NanoLuc substrate was added to the living cells (40 μl/well, containing 1.0 μl of NanoLuc substrate stock from Promega, Madison, WI, USA). Thereafter ...
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bioRxiv - Biochemistry 2024Quote: ... Sequencing-grade modified trypsin was purchased from Promega (Madison, WI). Water was obtained from a Millipore Milli-Q system (Bedford ...
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bioRxiv - Biochemistry 2024Quote: ... After 10h of serum starvation cells were lysed using Dual-Glo Luciferase Assay System from Promega and firefly and renilla luciferase signals were measured using a Synergy HTX (BioTek ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2024Quote: ... NanoBRET™ NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) was added as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence was detected at 560 nm on GloMax Discover Microplate Reader (Promega). The raw fluorescence values were normalized to DMSO-treated cells (set as 100%).
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bioRxiv - Biochemistry 2024Quote: ... plates were equilibrated to RT for 15 min and CellTiter Glo reagent (Promega, Madison, WI, USA) was added followed by orbital shaking for 2 min ...
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bioRxiv - Biophysics 2024Quote: ... and following digest with sequence-grade trypsin (Promega) and lysyl endopeptidase (LysC ...
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bioRxiv - Bioengineering 2024Quote: ... the Firefly luciferase substrate (LAR II reagent, 100 µL/well, Promega Dual Glo kit, cat # E1960) was added to each well ...
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bioRxiv - Bioengineering 2024Quote: ... and purified using the PureYield MidiPrep System (Promega, Madison, WI).
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bioRxiv - Bioengineering 2024Quote: ... Measurements were taken every 20 min (225 min total) in Nunc™ F96 MicroWell™ 446 plates using a GloMax® navigator luminometer (Promega) with 5 s integration time and 2 s delay ...
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bioRxiv - Bioengineering 2024Quote: ... Bioluminescence measurements were taken by combining 25 µL of the sample solution with an equal volume of prepared buffer-reconstituted nluc substrate as detailed by the manufacturer (NanoGlo Luciferase Assay System; Promega). Measurements were taken every 20 min (225 min total ...
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bioRxiv - Bioengineering 2024Quote: ... The digested product was gel-extracted using the Wizard SV Gel and PCR Clean-Up Kit (Promega). The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... the UMP/CMP-Glo™ Glycosyltransferase Assay (Promega) was used to quantify the resulting UMP ...
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bioRxiv - Biochemistry 2024Quote: ... Cell viability assay was performed using CellTiter-Glo® 2.0 reagent (Promega, G9241) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 90 µL of 25 mM ammonium bicarbonate containing 0.5 µg of MS grade Trypsin/LysC (Promega) (enzyme/protein ratio of 1:20 (w/w) ...
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bioRxiv - Bioengineering 2024Quote: ... Polymerases and other molecular biology reagents were purchased from Promega. Lo bind RNA microcentrifuge tubes were purchased from Eppendorf.
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bioRxiv - Biophysics 2024Quote: ... EDTA-free protease inhibitor cocktail (Promega) and DNase ...
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bioRxiv - Biophysics 2024Quote: The fused peptide–mRNA was subsequently reverse transcribed using MMLV RT Rnase H-(Promega) for 1 h at 42 °C ...
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bioRxiv - Bioengineering 2024Quote: ... The Glucose-Glo and Lactate-Glo kits (Cat #’s J6021 and J5021, Promega, Madison, WI) were used to measure the apparent glucose and lactate concentrations in media samples according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 10 ng/µL trypsin (Promega Gold, V5280, Promega, USA)) is dispensed into each well ...
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bioRxiv - Biochemistry 2024Quote: ... followed by digestion with trypsin (Promega). Digested peptides were processed and used to determine the protein content of each corresponding sample as described in Wales et al ...
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bioRxiv - Biochemistry 2024Quote: ... 10 ng/µL trypsin (Promega Gold, V5280, Promega, USA)) is dispensed into each well ...
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bioRxiv - Bioengineering 2024Quote: ... the 1X Renilla-luciferase substrate mixed with a Stop & Glo buffer (100 µL/well, Promega Dual Glo kit, cat # E1960) was added ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of 1X passive lysis buffer (Promega Dual Glo kit) was added to each well and allowed to incubate for 2 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... and 10 µl of 0.1 µg/µl Trypsin/LysC Mix (Promega #V5073) was added ...
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bioRxiv - Biochemistry 2024Quote: ... and treated with passive lysis buffer (Promega) by freezing for 1 h at -80 °C and thawing at 37 °C for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... Fugene 6 was purchased from Promega (Madison, WI). Multiple plasmids were ordered or generated for this study ...
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bioRxiv - Biochemistry 2024Quote: ... the blotted proteins were developed using Nano-Glo® In-Gel Detection System (Promega) and scanned with a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... the PCR products of the Strep-Flag-tag and NLuc (Promega, cat # N1091) were inserted into the pHLmMBP-8 vector (Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with 50 mM Tris-HCl pH 8.0 and digested for 12 h at 37°C using sequencing grade trypsin (Promega cat#: V511A) at 1/100 the mass of total protein ...
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bioRxiv - Biochemistry 2024Quote: ... IPO9 ORF was cloned into the pFC32A Nluc CMV-neo Flexi® vector (Promega, cat # N1331) using Flexi® Cloning System (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... beads were resuspended in 100 μL of 50 mM TEAB and enzymatically digested overnight using sequencing-grade trypsin (Promega, V5111). Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... or K562 (Promega, Mass Spec-Compatible Human Protein Extract ...
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bioRxiv - Biochemistry 2024Quote: ... The remainder of the reaction was treated with 50 µg/mL trypsin (Promega) and incubated at room temperature for the indicated times ...
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bioRxiv - Biochemistry 2024Quote: ... 4 µL furimazine (0.36% vol/ vol diluted in BRET assay buffer, Promega) was added before readout on ClarioStar.
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bioRxiv - Biochemistry 2024Quote: ... Untagged CDK2 expression plasmid was cloned by PCR with CDK2-NLuc plasmid (Promega) as the template ...
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bioRxiv - Biochemistry 2024Quote: The assays were conducted using the DNA-PK Kinase Enzyme System (Cat # V4106, Promega), following the provided instructions ...
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bioRxiv - Biochemistry 2024Quote: ... to reach 1% vol/vol as recommended by Promega, then mixed with YZ-01-A (final DMSO 1% vol/ vol ...
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bioRxiv - Biochemistry 2024Quote: ... using ADP-Glo Kinase Assay (Promega). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... NLuc-N112C-CCNE1-3xFLAG plasmids were cloned into PvuI and EcoRI digested NLuc-CDK4 plasmid (Promega) using gBlocks obtained from IDT ...
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bioRxiv - Biochemistry 2024Quote: ... Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega, E2620) on a Tristar 5 microplate reader (Berthold).
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bioRxiv - Biochemistry 2024Quote: ... samples were digested using MS-grade trypsin (Promega) at 37 ℃ overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Normalized cell lysate was first mixed with vivazine (Promega) to reach 1% vol/vol as recommended by Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The expression plasmids were transiently transfected by using FuGENE HD (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Forty-eight hours later, cells were treated with Thapsigargin (100nM, 4h) and cells were resuspended in passive lysis buffer (Promega). Firefly and Renilla luciferase activities were measured using a dual-luciferase reporter assay system (#E1960 ...