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Citations for Promega :
1551 - 1600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Sequencing-grade trypsin (Promega) was added at a 1:100 ratio relative to total protein in the lysate and digestion allowed to proceed overnight at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Add 20 µl/well of 1x luciferase cell culture lysis reagent (Promega). Add 100 µl of Luciferase Assay reagent (Promega ...
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bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
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bioRxiv - Immunology 2024Quote: ... Add 100 µl of Luciferase Assay reagent (Promega) to each well ...
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bioRxiv - Immunology 2024Quote: ... and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol and measured using GloMax Discover (Promega).
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bioRxiv - Immunology 2024Quote: ... 1 µg of trypsin (Promega) was added for overnight digestion at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... proteolytic digestion was performed by adding Trypsin (0.1 μg/μL in 1 mM HCl, Sequencing Grade Modified Trypsin, Promega) at a ratio of 50:1 (w:w ...
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Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: ... Such cDNA samples were applied as templates for RT-qPCR using 2× GoTaq® qPCR Master Mix (Promega). The reactions were performed on the CFX96TM system (BIO-RAD ...
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Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: Genomic DNA from different isolates was obtained from pelleted overnight cultures using the Wizard Genomic DNA purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then used in a one-step RTqPCR (Promega) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The direct antiviral effect of betacyanin fractions was evaluated through the formation of viral CPE using the Viral ToxGloTM Assay (Promega, USA) per the manufacturer’s instructions with slight modifications ...
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bioRxiv - Microbiology 2024Quote: ... DNA concentrations were measured using the Quantus Fluorometer and QuantiFluor dsDNA System (Promega). Equimolar amounts (∼ 33 fmol ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then trypsin digested in 20µl 20mM ammonium bicarbonate and incubated with 10µl of 10ng/µl sequencing grade trypsin (Promega, cat# V5111) at 37°C for 3hrs at 1500rpm ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested on beads with 1 μg trypsin (Promega, UK) for 18 hours at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg of purified bacmid DNA were transfected into 1×106 Sf9 cells in a well of a 6-well plate using 6 μL of FuGENE HD transfection reagent (Promega). After 7 days of incubation at 27°C ...
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bioRxiv - Microbiology 2024Quote: ... Products and substrate molecules were analyzed by LC-MS or with NAD/NADH-Glo kit (Promega).
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bioRxiv - Microbiology 2024Quote: ... Lysates were analyzed by LC-MS or with the NAD/NADH-Glo kit (Promega). B ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the GoTaq polymerase (Promega) and a StepOnePlus real-time PCR device (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: The copy number of re-integrant strains was determined by extracting the genomic DNA and performing quantitative PCR (qPCR) using GoTaq polymerase (Promega) and a StepOnePlus real-time PCR machine (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... and anti-mouse IgG HRP conjugate (Promega, W4021) as the secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... baylyi ADP1 was first digested with RQ1 DNase (Promega, WI, USA), followed by reverse transcription to complementary DNA (cDNA ...
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bioRxiv - Microbiology 2024Quote: ... and the luciferase activity was measured using the dual-luciferase reporter assay (Promega, cat# E1910). For experiments involving the transfection of viral proteins (Fig ...
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bioRxiv - Microbiology 2024Quote: ... The Dual-Glo Luciferase Assay System (Promega) was used to determine the relative activity of firefly luciferase to Renilla luciferase ...
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bioRxiv - Microbiology 2024Quote: ... 20 µg of the plasmid was co-transfected with 2.5µg of pGL4.75 Renilla luciferase control vector (Promega) into DT40 AIDR/puro UNG-/- cells ...
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bioRxiv - Microbiology 2024Quote: ... the target cells were incubated with 1:250 diluted EnduRen live cell substrate (Promega). After detachment ...
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bioRxiv - Microbiology 2024Quote: ... The Nano-Glo live cell reagent (Promega) was used as advised by manufacturer to determine effector translocation into RAW LgBiT macrophages ...
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bioRxiv - Microbiology 2024Quote: ... in vitro transcription was carried out using the Ribomax large-scale RNA production systems from Promega, and capping using the ScriptCap m7G capping system (Cellscript).
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity was assessed via the Renilla luciferase kit (Promega). In brief ...
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bioRxiv - Microbiology 2024Quote: ... Subsequently Trypsin/LysC solution [100 ng/μL 1:1 Trypsin (Promega):LysC (FujiFilm ...
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bioRxiv - Microbiology 2024Quote: ... named rpsL*) was amplified by PCR with primers BlD-LLcfusARpsL/BlD-LLldacARpsL and cloned into the pGEM-T Easy vector (Promega), yielding plasmid pGEM-rpsL* ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... transferred to a PCR clean-up column (Promega, A9282) and centrifuged ...
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bioRxiv - Microbiology 2024Quote: HeLa cells were transfected with plasmids encoding ByeA or ByeAC197A according to the FuGENE transfection protocol (FuGENE® HD Transfection Reagent, Promega Cat. E2311) as advised by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... while the DNA was quantified using the GloMax Plate Reader System (Promega) using the QuantiFluor® dsDNA System (Promega ...
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bioRxiv - Microbiology 2024Quote: ... we used the Nano-Glo HiBiT lytic detection system (Promega, Catv N3030). To determine the linear range of the luminescence signal we performed a dilution series with different numbers of bacteria ...
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bioRxiv - Microbiology 2024Quote: ... using the QuantiFluor® dsDNA System (Promega) chemistry ...
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bioRxiv - Microbiology 2024Quote: ... Each 25 µL reaction contained 1x GoTaq Colourless Master Mix (Promega, United States) and 0.3 µM Primers (invA_1869F ...
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bioRxiv - Microbiology 2024Quote: ... the nLuc activity of each antigen was adjusted to comprise 107 RLU per second (RLU/s) in a volume of 50 µL (NanoGlow, Promega, Madison, Wisconsin, USA).
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA was isolated from overnight cultures using Wizard® Plus SV Minipreps DNA Purification Systems (Promega, Wisconsin, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Briefly, 100 µl elution buffer 1 (100 mM ammonium bicarbonate, 1 µg trypsin (Promega)) was added to each sample ...
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bioRxiv - Immunology 2024Quote: ... qPCR targeting the β-globin gene was performed for each sample and evaluated alongside a β-globin calibration curve (human gDNA, cat# G1521, Promega).
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bioRxiv - Immunology 2024Quote: DNA was extracted from 200 mg of each fecal sample via bead beating and column purification using a Maxwell 16 MDx Instrument (Promega, USA), as previously described (Sikder ...
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bioRxiv - Immunology 2024Quote: RNA from cells was isolated using the ReliaPrep RNA Miniprep System (Promega). RNA was reverse transcribed using UltraScriptTM cDNA Synthesis Kit (PCR Biosystems ...
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bioRxiv - Immunology 2024Quote: Cell viability was determined by the CellTiter-Glo Luminescent Cell Viability Assay (Promega, G7571), the Cell Counting Kit-8 (CCK-8 ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Immunology 2024Quote: Total RNA extracted from BMDCs using the ReliaPrep RNA Cell Miniprep System (#Z6012, Promega, Madison, WI, USA) was reverse-transcribed to cDNA with ReverTra Ace qPCR RT Master Mix (#FSQ-201 ...
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bioRxiv - Immunology 2024Quote: ... A mix containing 1 µL of 10 mM dNTP (Promega), 5 µL of 0.25 µM NexteraXT primer index ...
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bioRxiv - Immunology 2024Quote: ... 0.4 µL of 10 mM dNTP mix (Promega), 0.4 µL of primers mix alpha ...
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bioRxiv - Immunology 2024Quote: ... One μg PCR product was used as the specific-template for synthesizing of dsRNA in vitro by using the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The concentration of dsRNA was quantified at 280 nm using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega, Dübendorf, Switzerland).