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Citations for Promega :
1801 - 1850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 5 µL of the schizont culture was aliquoted into white 96 well luminometer plates to which 45 µL of 1x NanoGlo Lysis buffer with 1:1000 NanoGlo substrate (Promega, USA) was added ...
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bioRxiv - Cell Biology 2024Quote: ... following manufacturer instructions (Promega, Cat#TM494). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell growth was assessed by CellTiter-Glo (Promega) assay and Cristal violet assay according to manufactures instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated RNAs were treated with RNase-free DNase I (Promega), purified on G-50 MicroSpin columns (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: 20×106 cells were collected in cold PBS by scraping and lysed in RIP buffer (251mM Tris-HCl pH 7.4, 1501mM KCl, 5mM EDTA, 0.5% Nonidet P-40, 201U1ml−1 RNAsin (Promega), 0,51mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2024Quote: ... RNAs were incubated with [5’-32P] pCp and T4 RNA ligase (Promega, M1051) O/N at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... RNasin ribonuclease inhibitor (Promega, N261B)) and disrupted with a Bioruptor Sonicator by sonication (2 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction mixture was then transformed at a ratio of 1:10 (reaction volume/volume competent cells) into HB101 competent cells (Promega #L2011). Spectinomycin-resistant colonies were screened by restriction digestion of miniprep DNA and subsequently validated by gel electrophoresis in a 0.8% agarose gel and by DNA sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... the samples were diluted with seven volumes of 50 mM ammonium bicarbonate and incubated for 16 h at 37 °C with 400 ng of trypsin (ratio 1:50; “Trypsin Gold”, Promega).
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bioRxiv - Cell Biology 2024Quote: ... and ERα were cloned into the Promega NanoBRET vectors (Promega N1811) using Gibson Assembly (NEB E2611 ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were quantified using the QuantiFluor™-ST Blue Fluorescence Quantification System (Promega). After Illumina PE250 library construction ...
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bioRxiv - Systems Biology 2024Quote: ... UA buffer was exchanged with 50 mM ammonium bicarbonate and sequencing-grade trypsin (Promega, Madison, WI, USA) was added at an enzyme to protein ratio of 1:50 ...
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bioRxiv - Systems Biology 2024Quote: ... according to the manufacturer’s instructions (Promega, Madison, WI). dsRNA targeting the green fluorescent protein (GFP ...
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bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
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bioRxiv - Systems Biology 2024Quote: ... we incorporated polyethylene glycol (PEG 8000; Promega) at a concentration of 10% w/v in each reaction ...
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bioRxiv - Systems Biology 2024Quote: ... 2.5 μL of DNase (1 U/μL, T7 RiboMAX; Promega) was added to the reaction and further incubated for 30 minutes at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... and 50 nL of 0.04 ng nL-1 trypsin (Promega) in 0.1 M HEPES buffer and incubated at 37 °C for 10 h ...
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bioRxiv - Systems Biology 2024Quote: ... Residual DNA was removed using the RQI RNAse-Free DNase kit (Promega). Total RNA was then quantified using the Agilent RNA 6000 nano kit (catalogue number ...
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bioRxiv - Microbiology 2024Quote: ... the genomic DNA of the mutants was isolated using a Wizard Genomic DNA Purification Kit (Promega). The isolated genomic DNA was analyzed by agarose electrophoresis and quantified using a NanoDrop spectrophotometer ...
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bioRxiv - Systems Biology 2024Quote: ... protein digestion was performed overnight by addition of 50 nL of 0.01 ng nL-1 Lys-C (MS grade, Promega, Madison, USA) and 50 nL of 0.04 ng nL-1 trypsin (Promega ...
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bioRxiv - Systems Biology 2024Quote: ... The assay was repeated three times and was conducted using the Caspase Glo® 3/7 assay kit (Promega, USA) based on the manufacturer’s recommendations.
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bioRxiv - Synthetic Biology 2024Quote: ... and Nluc reporter was quantified with Nano-Glo Luciferase Assay System (Promega). The substrate was prepared according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: Signal of the Fluc reporter was quantified with the Renilla Luciferase Assay System (Promega). The substrate was prepared according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Then 30 µl of premixed Nano-Glo substrate/buffer (Promega #N1138 and Promega #N1128 prepared following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... was performed with the Nano-Glo® HiBiT Lytic Detection System (Promega). Precultures and cultures for the measurements were grown in 96-well plates in the Tecan infinite f200pro microplate reader as described above ...
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bioRxiv - Synthetic Biology 2024Quote: Signal of the Fluc reporter was quantified with the ONE-Glo Luciferase Assay System (Promega) and Nluc reporter was quantified with Nano-Glo Luciferase Assay System (Promega) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Then 30 µl of premixed Nano-Glo substrate/buffer (Promega #N1138 and Promega #N1128 prepared following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the lentiviral packaging plasmids pMD2.G (170ng) and pCMV-dR8.91(1.33μg) using 10μl of Fugene HD (Promega #E2312) according to manufacturer protocols ...
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bioRxiv - Synthetic Biology 2024Quote: ... Isolated amplicons were cloned in pGEM-T-Easy (Promega, USA) and sequenced (Macrogen ...
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bioRxiv - Microbiology 2024Quote: ... amoA gene amplicons were generated using 0.6 ng DNA template with GoTaq DNA polymerase (Promega) and 0.5 μM primers (CamoA_19F [5’-ATGGTCTGGYTWAGACG-3’] and TamoA_632R-4 [5’-GCKGCCATCCATCKRTANGTCCA-3’] ...
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bioRxiv - Synthetic Biology 2024Quote: Pantropic VSV-G pseudotyped lentivirus was produced via transfection of Lenti-X 293T cells with a modified pHR’SIN:CSW transgene expression vector and the viral packaging plasmids pCMVdR8.91 and pMD2.G using Fugene HD (Promega #E2312). Primary T cells were thawed the same day ...
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bioRxiv - Systems Biology 2024Quote: ... 0.001% Digitonin (Promega)) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Four microliters of CFPS sample were added to 30 μL of ONE-Glo Luciferase Assay System (Promega, Madison WI, USA) in a white 96-well plate (Costar 3693 ...
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bioRxiv - Systems Biology 2024Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies ...
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bioRxiv - Synthetic Biology 2024Quote: ... in live cell substrate (LCS) dilution buffer (Nano-Glo® assay, Promega) was added to each well ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2 μL of 20-fold diluted furimazine stock (Promega) in live cell substrate (LCS ...
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bioRxiv - Systems Biology 2024Quote: ... 25μL of CellTiter-Glo (Promega Corp, Madison, WI) were added to each well ...
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bioRxiv - Systems Biology 2024Quote: ... followed by a 3:1 dilution with 100mM ammonium bicarbonate and addition of 2µg sequence-grade trypsin (Promega). Samples were digested at room temperature overnight and acidified with formic acid (final concentration 1%) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Bound proteins were eluted with elution buffer 1 (50 mM Tris-HCl pH 7.5, 2 M urea, 5 ng/µL sequencing grade modified trypsin (Promega, Madison, WI, USA), 1 mM DTT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell survival was measured three days later using CellTiter-Glo luminescent cell viability assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... target cDNA fragments were amplified with the primers listed in Table 1 and ligated into pGEM T-Easy vector (Promega, Madison, WI). The cDNA fragments were PCR amplified with M13 primers and purified with the FastGene Gel/PCR Extraction Kit (Nippon Genetics ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 U/ml of RNasin (Promega, cat# N2611). Finally ...
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bioRxiv - Synthetic Biology 2024Quote: ... and cDNA was synthesized using the GoScript reverse transcription kit A5001 (Promega), according to the manufactures’ instructions ...
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bioRxiv - Synthetic Biology 2024Quote: GoTaq Green (Promega) PCR was used for direct PCR from yeast and bacterial colonies when doing colony PCR for screening ...
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bioRxiv - Neuroscience 2024Quote: ... Protein digestion was carried out with sequencing grade modified trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Neuroscience 2024Quote: ... 48 hours post-transfection both Firefly and NanoLuciferase signals were measured using the Nano-Glo Dual Luciferase Assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... the T7 RiboMAX Large-Scale RNA Production System (Promega, Madison, WI, USA) was used and the synthesized mRNAs were purified with NucleoSpin RNA Plus (Takara) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and luminescence was measured using Bright-Glo substrate and lysis buffer (Promega) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Leaf tissue was collected from plants for high-molecular weight DNA extraction (Wizard HMW DNA Extraction, Promega, Madison, WI). We used flow cytometry to estimate genome size relative to Conyza canadensis and Solanum lycopersicum ...
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bioRxiv - Synthetic Biology 2024Quote: ... a stable Firefly Luciferase (Luc2, Promega) has been used in order to compare it with the original biosensor.