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Citations for Promega :
1701 - 1750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Firefly Luciferase and Renilla Luciferase expression were measured using the Dual Luciferase Assay (Promega) on a Glomax Explorer plate reader (Promega) ...
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bioRxiv - Synthetic Biology 2024Quote: ... per well and then 25 μl PBS and 25 μl of the Nano-Glo® Luciferase Assay System reagent (Promega) were added ...
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bioRxiv - Physiology 2024Quote: ... (Promega #E1941, diluted 1:5 with water). Dual-Glo Luciferase assays were performed to measure Firefly and Renilla luciferase activity (Promega #E2940) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cells were transiently transfected using Fugene HD (Promega #E2311), according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were transfected the following day with 1,000 ng of either the control plasmid or the BFP-miR-L plasmid using Fugene HD (Promega #E2311) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Trypsin (Promega) was added at 1:50 (w/w ...
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bioRxiv - Systems Biology 2024Quote: ... Transfection was performed with FugeneHD (Promega, UK) at a 3:1 ratio to DNA in accordance with the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... coagulans DSM 1 = ATCC 7050 was extracted using Wizard Genomic DNA Purification Kit (Promega) according to the protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The activities of luciferase were measured using Dual-Luciferase® Reporter Assay System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was obtained from 1 μg of total RNA pretreated with DNase I (Promega) by reverse transcription reaction using ImProm-II(TM ...
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bioRxiv - Plant Biology 2024Quote: ... and cloned into pGEM-T easy (Promega) vector ...
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bioRxiv - Plant Biology 2024Quote: ... curassavica leaves using the Wizard® Genomic DNA Purification Kit (Promega, Madison WI, USA). The quantity and quality of genomic DNA was assessed using a Qubit 3 fluorometer (Thermo Fisher ...
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bioRxiv - Plant Biology 2024Quote: ... These plasmids were subsequently amplified and extracted by PureYield™ Plasmid Miniprep System (Promega), and electroporated into Agrobacterium tumefaciens GV3101 electrocompetent cells ...
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bioRxiv - Cancer Biology 2024Quote: ... An MTS assay (CellTiter 96® Promega) was then performed as above to measure the numbers of surviving cells present ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular proliferation was assessed via MTS assay (CellTiter 96® Promega) at 24 ...
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bioRxiv - Zoology 2024Quote: ... The dsRNA cDNA templates were ligated into the pGEM T-Easy vector (Promega, Madison, WI, United States) and subcloned into the dsRNA-producing vector L4440 (Fire et al. ...
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bioRxiv - Zoology 2024Quote: ... 0.25 μl Taq (Gotaq flexi, Promega), 1 μl template ...
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bioRxiv - Zoology 2024Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Microbiology 2024Quote: ... Tat and Rev and plasmid encoding spike of SARS-CoV-2 variants Delta or Omicron were transfected in HEK 293T cells using Fugene6 reagent (Cat. No. E2691, Promega) following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... and CoaAMtb_DM enzymatic activity was measured using ADP-Glo Luminescent assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein precipitate was air-dried and resuspended in 200ul of 25mM ammonium bicarbonate solution and 3ul of trypsin (Promega) and digested overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... following the addition of BrightGlo luciferase reagent (Cat. No. E2620, Promega). The dilution of the virus that showed ∼104 RLUs was used in the pseudovirus neutralization assay.
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bioRxiv - Cancer Biology 2024Quote: ... After three days of treatment cell viability was measured with CellTiter-Glo Assay (Promega) according to the manufacturer’s instructions and a microplate reader (CLARIOstar Plate Reader ...
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bioRxiv - Cancer Biology 2024Quote: ... were reverse transfected in a 96 well plate format with a custom siGENOME SMARTPool (Dharmacon) siRNA library as described previously72 using Lipofectamine RNAimax Transfection Reagent (Promega). Positive (siPLK1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted with the Maxwell RSC instrument using the RSC simplyRNA Cells Kit (#AS1390, Promega) after the indicated treatment ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was conducted with GoTaq qPCR master mix (Promega) for the desired genes and housekeeping genes of which the primer sequences are given in Table 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent cDNA synthesis was performed with GoScript Reverse Transcriptase kit (Promega). Finally ...
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bioRxiv - Microbiology 2024Quote: ... Cleared protein extracts were incubated with 200 μl of MagneHis solution (Promega, USA) overnight ...
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bioRxiv - Zoology 2024Quote: ... and 3% 20mg/ml Proteinase K (Promega). This digestion eliminates cellular material while leaving the spongin network intact ...
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bioRxiv - Neuroscience 2024Quote: ... the bead and protein mixtures were washed twice with 80% ethyl alcohol and once with 100% ACN before being resuspended in 95 µL NH4HCO3 prior to overnight on-bead digestion (enzyme–protein ratio of 1:20) at 1 000 rpm at 37 °C using modified porcine trypsin/Lys-C Mix (Mass Spectrometry Grade, Promega). The digestion was stopped using trifluoroacetic acid (TFA ...
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bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Zoology 2024Quote: RT-PCR was performed using AccessQuick RT-PCR System (Promega, France) in total reaction volume of 25 µl with 5 µl of RNA extracts and 400 nM of forward primer (5’-ATTCGGGAGGAGACGTGGTA-3’ ...
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bioRxiv - Zoology 2024Quote: ... The amplicons-containing were excised after gel-electrophoresis and extracted DNA by using Wizard SV Gel and PCR Clean-Up System (Promega). The amplicon DNAs were further analyzed for Sanger sequencing with the QuantumDye Terminator Cycle Sequencing Kit (Tomy Digital Biology ...
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using the ReliaPrep RNA Cell Miniprep System (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2024Quote: For RNA extraction we used the ReliaPrep RNA Cell Miniprep System (Promega Kit). For the preparation of the Column Wash Solution ...
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bioRxiv - Cancer Biology 2024Quote: ... Wizard Genomic DNA purification kits (Promega, Madison, WI, USA), or Quick-DNA Miniprep Kits (Zymo Research ...
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bioRxiv - Cancer Biology 2024Quote: ... At the end of treatment cells were incubated O/N with CellTiterBlue reagent (Promega, cat. G8080). The day after ...
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bioRxiv - Bioengineering 2024Quote: ... The reactions were performed using the S30 T7 High-Yield Protein Expression System (Promega). 3 μl of peptide solution were added to reaction mixtures to get a final concentration respectively of 32 μg/ml or 512 μg/ml in a final volume of 15 μL in nuclease-free PCR tubes ...
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bioRxiv - Bioengineering 2024Quote: ... was quantified using the commercial kit Renilla-Glo Luciferase Assay System (Promega). Mixing 2.5 μl of reaction mixtures and 97.5 μl of the provided buffer ...
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bioRxiv - Systems Biology 2024Quote: ... RNA was refolded by adding 88.8 μL 300 mM sodium cacodylate and 1 μL RNasin Plus (Promega N2611) and 10 min incubation at 37 °C ...
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bioRxiv - Systems Biology 2024Quote: ... reactions were immediately halted with ice-cold Passive Lysis Buffer (Promega E1941). Firefly luciferase and nano luciferase levels were then measured using Bright-Glo (Promega E2620 ...
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bioRxiv - Systems Biology 2024Quote: ... and Nano-Glo (Promega N1130), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... 100 μl of trypsin digestion solution (Promega, 5 μl of reconstituted trypsin was dissolved in 100 μl 50 mM Tris-HCl buffer pH 8 ...
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bioRxiv - Biophysics 2024Quote: ... (Promega). Samples were digested with trypsin and digestion was stopped by the addition of 5% formic acid ...
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bioRxiv - Systems Biology 2024Quote: ... Firefly luciferase and nano luciferase levels were then measured using Bright-Glo (Promega E2620) and Nano-Glo (Promega N1130) ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then treated with DNAse (RQ1 RNAse-Free DNAse-Promega), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... by reverse transcription reaction using ImProm-II(TM) Reverse Transcriptase (Promega) with random hexamers primers ...
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bioRxiv - Plant Biology 2024Quote: ... we employed a luciferase-based GTPase assay utilizing the GTPase-GloTM Assay Kit (Promega). The assay was conducted following the guidelines provided by the manufacturer ...