Labshake search
Citations for Promega :
1301 - 1350 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... corresponding samples (PSD-95 and MPP2) were incubated overnight at 37°C with 50 ng trypsin (sequencing grade modified, Promega) in 25 µl of 50 mM ammonium bicarbonate in the presence of heavy water (Campro Scientific GmbH ...
-
bioRxiv - Microbiology 2021Quote: ... Each aliquot was diluted eight-fold with 50 mM ABC and 10 μg of sequencing grade modified trypsin (Promega #V5113) was added ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 10 μg of each protein was then boiled in PCR tubes at 100 °C for 5 min and allowed to cool to room temperature before 1 μg mass spectrometry-grade trypsin (Trypsin Gold from Promega) was added to each sample ...
-
bioRxiv - Systems Biology 2020Quote: ... to a final concentration of 1% DOC before overnight digestion at 37 °C with lysyl endopeptidase (Wako Chemicals) and sequencing-grade porcine trypsin (Promega) at an enzyme-substrate ratio of 1:100 for both ...
-
bioRxiv - Physiology 2020Quote: ... reduced (using DTT) and alkylated (using iodoacetamide) and subjected to enzymatic digestion with sequencing grade Trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
-
bioRxiv - Bioengineering 2020Quote: ... In-solution protein digestion was carried out in a ratio of 1:25 w/w Trypsin/Lys-C (Mass Spectrometry Grade, Promega) to protein overnight at 37°C ...
-
bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
-
bioRxiv - Immunology 2021Quote: ... dithiothreitol was added to a final concentration of 50 mM and proteins were digested overnight with sequencing grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:50 at 37 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... Twenty microliters of HPLC-grade water were added to reduce urea concentration and trypsin digestion was performed using a 1:20 (enzyme:protein) ratio of sequencing grade modified trypsin (Promega, V5111) and 18 h incubation at 37 °C on a ThermoMixer (Eppendorf ...
-
bioRxiv - Biochemistry 2021Quote: The samples were reduced and digested in 25 μL co-IP digest buffer (50 mM Tris-HCl, 5 ng/μL trypsin (sequencing grade, modified, Promega), 2 M urea ...
-
bioRxiv - Biochemistry 2021Quote: A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using FASP methods [24] ...
-
bioRxiv - Biochemistry 2021Quote: ... 55 mM IAA at room temperature for 30 min in the dark and digested at 37°C for 16 h with 2 μg porcine trypsin (sequencing grade, Promega). The resultant tryptic peptides were extracted in 40% ACN/0.1% TFA into fresh Protein LoBind® microtubes ...
-
bioRxiv - Biochemistry 2020Quote: ... three times with 250 μl of 50mM ammonium bicarbonate (ABC) buffer and digested for 16 hours with sequencing-grade modified trypsin (Promega) in 200 μl ABC buffer at 37 °C (enzyme to protein ratio 1:50) ...
-
bioRxiv - Cell Biology 2021Quote: ... the whole lane was cut in 7 bands and digested, as described (Shevchenko et al, 1996) with sequencing-grade trypsin (Promega). For the ubiquitination analysis ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were diluted nine-fold with 50 mM ammonium bicarbonate and digested overnight with trypsin (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 75:1.
-
bioRxiv - Microbiology 2021Quote: ... Protein samples were diluted 10-fold in 50 mM Tris-HCl and digested with Sequencing Grade Modified Trypsin (Promega - V5111) at a Protein:Trypsin ratio 50:1 overnight ...
-
bioRxiv - Microbiology 2021Quote: ... After 30 min at 60°C the samples were cooled to room temperature and 4 μg of Sequencing Grade Trypsin (Promega) for digestion were added ...
-
bioRxiv - Microbiology 2021Quote: ... samples were washed-dried once again and digested overnight at 37°C using mass spectrometry grade Trypsin gold (Trypsin Gold, Promega) at a concentration of 12.5 ng/μl in 50 mM ammonium bicarbonate and 10 mM CaCl2 ...
-
bioRxiv - Microbiology 2020Quote: ... alkylated with iodoacetamide (IA) 55 mM in BCA 50mM (30 min) and enzymatically digested with Trypsin Gold Mass Spectrometry Grade (Promega) for 10 h at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...
-
A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Proteins were digested with endoproteinase LysC (Wako Laboratories) for 2 h at 25 °C followed by overnight digest with sequencing-grade trypsin (Promega) at 25 °C (enzyme-to-substrate ratios of 1:50) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 50 mM ammonium bicarbonate the gel-pieces were lyophilised and in-gel digestion performed by incubation with sequencing-grade trypsin (Promega) in 10% acetonitrile ...
-
bioRxiv - Cell Biology 2021Quote: ... On-bead digestion was performed by overnight incubation at 37°C with 2 μg of mass spectrometry grade trypsin (Promega). The following morning an additional 2 μg of trypsin was added to each sample and incubated at 37°C for 4-6 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... and digested overnight at 37°C with 1:50 enzyme:substrate ratio (wt/wt) of sequencing grade trypsin (Promega, Madison, WI) as described 59 ...
-
bioRxiv - Cell Biology 2020Quote: ... gel pieces were reswelled in 60 μL of 50 mM NH4HCO3 buffer containing 10 ng/μL of trypsin (modified porcine trypsin sequence grade, Promega) incubated for one hour at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
-
bioRxiv - Biochemistry 2022Quote: ... before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg /μL sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off ...
-
bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns with a 3 kDa molecular weight cutoff and digested separately overnight using trypsin chymotrypsin or Glu-C (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
-
bioRxiv - Microbiology 2022Quote: ... The samples were diluted with 225 μL of 57.6 mM NH4HCO3 containing 1 mM TCEP and the mixtures treated with 1 μg of sequencing grade modified trypsin (Promega), incubated at 37 °C overnight and then acidified by addition of formic acid to 0.1 % v/v and stored at −20 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... the concentrated sample was removed from the ultra-centrifugal filter and a 1:50 ratio of mass spectrometry grade Trypsin Gold (Promega) added to the sample which was incubated with shaking for > 12 hours at 37 °C in a thermal heat block ...
-
bioRxiv - Cell Biology 2022Quote: ... and dried in at 37 °C before overnight digestion with 30 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Gel pieces were dried in a speed-vac for 20 min and were re-hydrated in 50 µl of sequencing grade trypsin (Promega, 1.3 ng/µl solution in 10 mM ammonium bicarbonate / 10% acetonitrile) ...
-
bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
-
bioRxiv - Microbiology 2022Quote: ... Gel bands were manually excised and cut in pieces before being subjected to digestion using modified trypsin (Promega, sequencing grade) as previously described (51) ...
-
bioRxiv - Microbiology 2022Quote: ... were stacked in the top of a 12% SDS-PAGE resolving gel before in-gel digestion using modified trypsin (sequencing grade, Promega), as previously described (Casabona et al. ...
-
bioRxiv - Plant Biology 2021Quote: ... The gel plugs were then dried using a speedvac and digested with sequence-grade modified trypsin (40 ng for each band) (Promega) in 25 mM NH4HCO3 overnight at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... protein lanes were cut into 10 pieces, destained (600 rpm, 37 °C, 200 mM NH4HCO3 in 30% acetonitrile) and digested with trypsin (sequencing grade, Promega) overnight at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... The samples were diluted with four volumes of 50 mM ammonium bicarbonate (ABC) and digested overnight with 1µg of sequencing grade modified trypsin (Promega, USA). All digestion steps were performed at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... All samples were digested overnight at 37 °C using a Trypsin/Lys-C Mix (mass spec grade, Promega, Madison, WI) (enzyme/substrate of 1:100 w/w ...
-
bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade, Promega). Supernatants were dried in a vacuum centrifuge and resuspended in 0.1% TFA ...
-
bioRxiv - Biochemistry 2021Quote: ... Digestion was performed by adding 50 mM NH4HCO3 with 12 ng/μl sequencing-grade modified trypsin (Promega, Madison, WI, USA), incubation on ice for 4 h before overnight incubation at 37 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... and 20 mM iodoacetamide respectively and washed again twice with 200 μl of 100 mM aqueous TEAB before incubating with 2 μg of trypsin (proteomics grade, Promega) at 37°C for 4h ...
-
bioRxiv - Biochemistry 2021Quote: Urinary proteins (100 μg of each sample) were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
-
bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and pellets were resuspended in 100 μl of 100 mM triethylammonium bicarbonate (TEAB) and subsequently digested overnight at 37 °C by adding 1.1 μg of sequencing-grade modified porcine trypsin (Promega, V5113). TMT reagents were reconstituted in 40 μl of anhydrous acetonitrile ...
-
bioRxiv - Microbiology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately overnight using trypsin chymotrypsin or alpha lytic protease (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
-
Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...