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Citations for Promega :
1251 - 1300 of 1768 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... In-solution protein digestion was performed using Lys-C (Wako Chemicals, Neuss) and trypsin (Promega). For analysis of the input samples ...
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bioRxiv - Molecular Biology 2021Quote: ... chymotrypsin in a chymotrypsin/protein ratio of 1/20 (w/w) (Promega Cat. No. V1061) and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein pellets were resuspended in 50mM ammonium bicarbonate in the presence of 0.0015% ProteaseMAX (Promega) and total protein amount was estimated with Pierce BCA assays (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: Proteins on beads were digested overnight at 37°C with trypsin (Promega, Madison, WI, USA) in a 25-mM NH4HCO3 buffer (0.2µg trypsin in 20µL) ...
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bioRxiv - Microbiology 2020Quote: ... PacC222 and PacC80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were digested using 4 μg of trypsin (sequencing grade, low autolysis trypsin; Promega), at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... the proteins were digested with 0.2 µg of sequencing-grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Developmental Biology 2022Quote: ... The 50μg of proteins were then trypsin digested overnight at 37 °C (1:25; Promega) using the filter aided sample preparation (FASP ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested by 0.2 μg modified grade porcine trypsin (Promega, Co., Madison, WI) during overnight incubation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein synthesis was performed in the presence of [35S]methionine in rabbit reticulocyte lysate (Promega). Coupled translation/translocation reactions of 20 μl contained 16.5 μl of reticulocyte lysate ...
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bioRxiv - Molecular Biology 2022Quote: One-hundred μl protein expression reaction of TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIXHALO-bZIP plasmids and 1500 ng pIXSBP-bZIP plasmids were incubated for 3 h at 30°C ...
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bioRxiv - Plant Biology 2022Quote: In vitro translation was carried out using a wheat germ protein expression system (Promega, USA) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... Eluc activity and protein concentration in supernatant were measured using the Luciferase Assay System (Promega) and the Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... We further used the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega) to express the four TFs-HaloTag fusion protein ...
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bioRxiv - Microbiology 2023Quote: ... for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega) for determination of cell viability/ATP concentration ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples eluted with biotin were digested in-solution with 1 ug Trypsin/LysC (Promega) overnight at 37°C ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts of equal protein amounts were first subjected to proteolysis with trypsin (V5113, Promega) at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 to 24 hours on bead with sequencing grade trypsin (Promega) in 50 mM HEPES buffer at 1:50 trypsin to protein ratio ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were extracted by methanol-chloroform precipitation and digested with 1 μg of trypsin (Promega) in 100 mM EPPS (pH 8.0 ...
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bioRxiv - Genomics 2023Quote: Proteins were expressed in rabbit reticulocyte lysate (TNT T7 Quick Coupled Transcription/-Translation System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Bound protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega). To quantify immobilized DNA in wells without protein ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were resuspended in the digestion buffer as previously described and trypsin (Promega, UK) digested to peptides74 ...
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bioRxiv - Genetics 2022Quote: Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega). If two proteins were to be tested together in a single binding reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and finally radiolabelled precursor proteins were synthesized with the Flexi rabbit reticulocyte lysate system (Promega). 35S-radiolabeled precursor proteins were incubated with isolated mitochondria in import buffer (3% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Biochemistry 2023Quote: ... coli and HeLa cells as well as of a commercially available yeast protein digest (Promega), The ratios of the peptide amount of each species within the three mixtures are given in Table S1.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2023Quote: Proteins were expressed using the TnT Coupled Wheat Germ Extract System with SP6 promoter (Promega). Protein expression was confirmed by Western Blot ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin, Promega, Fitchburg, WI). After stopping the digestion with 1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
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bioRxiv - Molecular Biology 2023Quote: ... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... For protein synthesis in reticulocyte lysate the TNT® Quick Coupled Transcription/Translation System (Promega) was used and performed according to the manufacture’s protocol employing 1 mM unlabeled methionine ...
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bioRxiv - Cell Biology 2024Quote: ... cells transfected with HALO-fusion protein were supplemented with 100 nM TMR HALO ligand (Promega). Cells were fixed at RT for 20 min in 3.7% formaldehyde (FA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then digested using 250 ng of trypsin enzyme (Sequencing Grade Modified Trypsin, Promega) and incubation for 1h at 37°C ...
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bioRxiv - Physiology 2022Quote: Capped cRNAs of the two Atlantic salmon PepT2 were synthesized by in vitro transcription using T7 RNA polymerase from cDNAs in pSPORT1 linearized with NotI and purified with Wizard SV Gel and PCR clean-up system (Promega Italia, Milan, Italy). The purified cRNAs were quantified by NanoDropTM 2000 Spectrophotomer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel pieces were resuspended in 20 μL l of trypsin solution (25 ng/ μL trypsin in 100 mM NH4HCO3) (Promega, Cat. No V5111) and incubated at 4°C for 20 min ...
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bioRxiv - Genomics 2019Quote: ... amplicons from each replicate were pooled and purified with the Wizard SV Gel and PCR Cleanup System kit (Promega Corporation, Madison, Wisconsin, USA).
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bioRxiv - Microbiology 2019Quote: ... Supplementary Table 4) and DNA sequencing of the products following clean-up (Wizard SV Gel and PCR Clean-Up System, Promega, Southampton, Hampshire, UK) using dye terminator chemistry (Eurofins ...