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Citations for Promega :
1201 - 1250 of 1768 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch, Irvine, CA) and cloned using pGEM-T Easy Vector System (Promega, Madison, WI). The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... reduced with 5 mM of DTT and alkylated with 11 mM iodoacetamide which was followed by in-gel digestion with trypsin (Promega, Madison, WI), chymotrypsin (Promega ...
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bioRxiv - Synthetic Biology 2022Quote: ... Amplified DNA products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega GmbH, Germany, Art. No. A9282). In all agarose gels ...
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bioRxiv - Synthetic Biology 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, 182 Madison, WI) at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... All PCR products were purified with an AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified using a QuantiFluor™-ST (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... all RT mixture of samples were pooled and purified with Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) according to the manufacture’s manual except for using 80% EtOH instead of Membrane Wash Solution ...
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bioRxiv - Genetics 2019Quote: The target genomic region was amplified using PCR and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The DNA sequence of the PCR fragment was determined using cycle sequencing with BigDye v3.1 reagents (Applied Biosystems ...
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bioRxiv - Physiology 2022Quote: ... agarose gel and the appropriate bands were cut out and purified according to the Wizard SV Gel and PCR Clean-Up System (Promega, Fitchburg, USA). A 20 min A-tailing step (72 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified using the Wizard SV Gel and PCR Clean-R19 purification systems following the instructions by the manufacturer (Promega, Madison, USA). The positive PCR amplicons were bidirectionally sequenced and gathered using BioEdit software (Hall ...
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bioRxiv - Microbiology 2023Quote: ... The 650 bp positive fragment was purified by Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... The 1800 bp positive amplicons was purified using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified with the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37 °C with Trypsin/Lys-C Mix (Promega, Charbonnières, France) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... proteins on the beads were digested by adding 400 ng trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were cleaved by overnight digestion in porcine trypsin (Sequencing Grade Modified, Promega, Wisconsin). The tryptic peptides were extracted by using 5% acetic acid in 50% acetonitrile and vacuum-dried by speedvac ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was carried out by adding 2 μg of trypsin solution (Promega, Charbonnières, France) to the alkylated proteins ...
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bioRxiv - Immunology 2021Quote: ... Total protein (100 μg) extracted from each sample was digested with Trypsin Gold (Promega, USA) with the ratio of protein:trypsin = 30:1 and incubated at 37 °C for 16 h ...
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bioRxiv - Cell Biology 2019Quote: ... and RXRA proteins were produced using the TNT® Quick Coupled Transcription/Translation System (Promega). Fluorescent probes were synthesized by Integrated DNA Technologies and corresponded to the LXRE1 sequence with IRDye 700 attached to the 5’ end ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested overnight at 37 °C using trypsin digestion (Sequencing Grade Modified Trypsin, Promega) at pH 8.0 using a protein to protease ratio of 100:1 ...
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bioRxiv - Biochemistry 2020Quote: A total of 100 μg protein was digested with Trypsin Gold (Promega, Madison, WI, USA) with the ratio of protein:trypsin = 30:1 at 37 °C for 16 h ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were subjected to on-filter proteolysis with Lys-C (Wako Chemicals) and trypsin (Promega). Tryptic peptides were eluted by centrifugation and acidified with trifluoroacetic acid prior to liquid chromatography-mass spectrometry (LC-MSMS ...
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bioRxiv - Physiology 2021Quote: ... the eluted proteins were digested with 10 μg/mL modified trypsin (Sequencing grade, Promega, USA) at 37 °C for 16 h ...
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bioRxiv - Plant Biology 2021Quote: ... Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000) (Promega). Cy3-labeled and unlabeled dsDNA probes were generated with oligonucleotides listed in Supplemental Table 5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared supernatant was added to a column containing HisLink Protein Purification Resin (V8823, Promega) and his-tag fusion proteins were purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Proteins in each well were digested with 1 μg of sequencing-grade trypsin (Promega Inc.) in 75 μl of 50 mM ammonium bicarbonate and incubated overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... On-bead protein digestion was performed by adding 1 µg of sequencing grade trypsin (Promega) to the samples ...
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bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
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bioRxiv - Systems Biology 2020Quote: ... Treated proteins were digested overnight at 37 °C using sequencing-grade trypsin (Promega, cat: V511C) at an enzyme-to-protein ratio of 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins extracted were then processed with a precipitation-based digestion protocol using trypsin (Promega) at a ratio of 1:20 as described previously(Collins et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and proteins were digested on-bead with trypsin (1.5 µg, in provided buffer, Promega Corp.) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: Halo fusion proteins were visualized in cells by staining with fluorescent Halo ligand (Promega GA1110). Cells were seeded on polyD lysine coated coverslips (neuVitro ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in the plugs where digested overnight with Trypsin (10μg/ml) (Promega, Madison, WI, USA) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Microbiology 2021Quote: ... Protein digestion was performed on beads using Trypsin Gold (MS grade; Promega, cat. no. V5280), as previously described in (Hughes et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were digested overnight at 37 °C using sequencing grade trypsin (100 ng; Promega) in a buffer containing 25 mM 4-ethylmorpholine acetate and 5% MeCN ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins on the beads were digested with 200 ng trypsin/Lys-C mix (Promega, V5072) for 16 h at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... the MBP-tag was cleaved off the fusion proteins by ProTEV Plus (Promega, Cat# V6102). Cleavage efficiency was typically ~70-90% at 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on-bead either with sequencing-grade trypsin (1:50) (Promega, Walldorf, Germany) for 4 h at 800 rpm and 42°C or thermolysine (1:50 ...
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were visualized by staining the membrane with TMB substrate (Promega; Cat.NO W4121).
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bioRxiv - Microbiology 2022Quote: ... Neutravidin-bound proteins were digested on bead with 400 ng Trypsin/Lys-C mix (Promega) at 37 °C overnight with shaking ...
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bioRxiv - Systems Biology 2022Quote: ... 500 µg of proteins were digested with trypsin/Lys-C mix (V5071 or V5072, Promega) overnight at 37 °C with a 1:50 enzyme to protein ratio ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were digested overnight at 37°C using 500 ng of trypsin (Trypsin Gold, Promega). Supernatants were kept and peptides remaining in gel pieces were further extracted with 1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Systems Biology 2019Quote: Cells were lysed and recombinant proteins were isolated using Magne® HaloTag® Beads (Promega) as previously described (15) ...
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bioRxiv - Molecular Biology 2019Quote: ... The protein samples were digested with 10 ng/μL Sequencing Grade Modified Trypsin (V5111, Promega) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins bound to beads were digested with 1 μg of Trypsin/Lys-C mix (Promega) for 3 h at 37 °C and then supplemented with and additional amount of 150 ng Trypsin/Lys-C for overnight digestion on an end-over-end rotation apparatus at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 μg of each proteins sample was digested by trypsin (cat No. V511, Promega, USA) using filter aided sample preparation (FASP) ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were digested overnight using trypsin protease (Sequencing Grade Modified Trypsin, Promega, Madison, WI, USA) in 1:50 w/w ratio ...
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bioRxiv - Immunology 2021Quote: ... Air-dried protein pellets were solubilized using 8M urea and Protease Max surfactant (Promega, V2071), then digested with trypsin (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 1 µg of trypsin (Promega). Thereafter ...