Labshake search
Citations for Promega :
1451 - 1500 of 1768 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: The total proteins (250 μg for each sample) were digested by FASP procedure using trypsin (Promega Corporation) as described previously60 ...
-
bioRxiv - Cell Biology 2023Quote: ... CTPS activity of purified CTPS1 and CTPS2 proteins was then analyzed using ADP Glo Kinase assay (Promega). CTPS1 and CTPS2 were diluted in assay buffer (50 mM Trizma ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight (21 h) at 37 °C with 0.1 μg/μL trypsin (sequencing grade, Promega) dissolved in 1 mM HCl ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein marker was purchased from Fermentas (Beijing) and M-MLV reverse transcriptase was purchased from Promega (Beijing). Homologous recombinant kit was purchased from Clone Smarter (Beijing) ...
-
bioRxiv - Biochemistry 2023Quote: ... The performance of the LC-MS system was controlled by analyzing a yeast protein extract digest (Promega). A total of eight datasets were collected and subsequently processed using three de novo sequencing algorithms CasaNova ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight at room temperature with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
-
bioRxiv - Bioengineering 2024Quote: ... Protein (25 µg per sample) was digested overnight at 37 °C with trypsin endoproteinase (Trypsin Gold, Promega), and the digested peptides were desalted using 1 cc C18 cartridges (Waters) ...
-
bioRxiv - Cell Biology 2024Quote: ... IMR90 exosome proteins trapped in the S-Trap columns were digested with trypsin (Promega, Madison, WI, # V5111) in digestion buffer (50 mM TEAB ...
-
bioRxiv - Plant Biology 2024Quote: Phosphorylation of HUB2 by SnRK2s was examined with ADP-Glo Protein Kinase Activity Assay Kit (Promega, V9101) with slight modifications (Kathania et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng of a trypsin/Lys-C mix (Promega) at 37°C overnight ...
-
bioRxiv - Developmental Biology 2024Quote: Abd-B proteins for EMSA were produced using the TNT coupled in vitro transcription/translation system (Promega). Protein production was estimated by labeling the proteins with 35S-methionine and found to be produced at similar amounts ...
-
bioRxiv - Microbiology 2024Quote: ... Protein digestion occurred overnight at 37 °C and 300 rpm with 1.5 µg sequencing grade trypsin (Promega). 0.5 pmol of the PierceTM Peptide Retention Time Calibration mix (Thermo Scientific ...
-
bioRxiv - Bioengineering 2022Quote: ... 3 mM CaCl2 at pH 6.4) 0.2 µg/μL protein was mixed with proteases trypsin (Promega sequencing grade), chymotrypsin (BD) ...
-
bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
-
bioRxiv - Neuroscience 2019Quote: ... The level of BDNF protein from tissue extracts was determined with the BDNF Emax® ImmunoAssay System (Promega) using the manusfacturer’s instruction ...
-
bioRxiv - Genomics 2019Quote: ... 35S labeled proteins were synthesized in vitro using the TNT T7 Quick for PCR DNA kit (Promega, L5540). Labeled proteins were incubated with MBP-tagged proteins in TNEN50 (50mM Tris pH7.5 ...
-
bioRxiv - Cell Biology 2019Quote: ... Samples were diluted two-fold with 20 mM HEPES pH 8.0 and proteins digested with Lys-C (Promega) at a protease/protein ratio of 1:100 (w/w ...
-
Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein was fluorescently labeled with 10uM TMR (dimer constructs) or 30uM TMR (monomer constructs, HALO-TMR; Promega) on beads for 1h at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: ... Samples were further diluted to a final urea concentration of 2 M and proteins digested with trypsin (Promega) (1/100 ...
-
bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... and the amount of the HiBiT protein was measured using the Nano Glo HiBiT Lytic Detection System (Promega). As a quantification standard for HiBiT proteins ...
-
bioRxiv - Microbiology 2021Quote: ATPase activity of the purified proteins was determined using two kits – Enliten ATPase assay (FF2000, Promega, Moscow, Russia) and malachite green phosphate assay (MAK307 ...
-
bioRxiv - Microbiology 2022Quote: ... and SV40 late poly(A) signal was amplified from pNL1.1.CMV[Nluc/CMV] (19.1 kDa NanoLuc protein, Promega). The PCR product was blunt end ligated to pAD1 digested with EcoRV ...
-
bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
-
bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
-
bioRxiv - Microbiology 2021Quote: ... sample-filters were incubated in a wet-cell adding 1:50 w/w trypsin: protein ratio (Promega, V5111) solubilized in 120 µl 50 mM TEAB ...
-
bioRxiv - Biochemistry 2021Quote: ... and S30 Premix Plus are components of the S30 T7 High-Yield Protein Expression System purchased from Promega. The pEXP5-PCFT plasmid is the PCFT construct shown in Fig ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted using 100 μL of Passive Lysis Buffer from Dual-Luciferase reporter assay systems (Promega, E1980) according to manufacturer’s instruction and assayed using FLUOstar Omega (BMG Labtech) ...
-
bioRxiv - Biophysics 2019Quote: ... Proteasomes were affinity purified with the bait protein GST-Ubl and magnetic beads (MagneGST™ Glutathione Particles, Promega) and eluted with purification buffer containing 25 mM reduced L-glutathione ...
-
bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
-
bioRxiv - Microbiology 2022Quote: ... Proteins on magnetic beads were digested overnight at 37°C with 1μl (0.2 μg/μL) of trypsin (Promega) in a 25-mM NH4HCO3 buffer per sample ...
-
bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
-
bioRxiv - Cancer Biology 2019Quote: ... Dilute proteins were digested overnight with 5 μl of 25 ng/μl trypsin gold (Promega, Madison, WI, USA) and desalted with a ZipTipC18 pipette tip (Millipore ...
-
bioRxiv - Molecular Biology 2019Quote: ... and PERP plus any interacting proteins were cleaved from the resin using 30 units of ProTEV enzyme (Promega) at room temperature for one hour (elution 1) ...
-
bioRxiv - Neuroscience 2019Quote: ... Resins were transferred to a new tube and proteins were digested with 0.1 µg Lys-C for 2h at 37°C and 0.5 µg trypsin (Promega) overnight at 37°C ...
-
bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...
-
bioRxiv - Cell Biology 2019Quote: ... In vitro translated (IVT-) proteins were synthesized using a TNT quick-coupled transcription-translation kit (Promega, Madison, WI). For synthesizing the Pav and PavDEAD proteins ...
-
bioRxiv - Plant Biology 2020Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega). Activation from the DHS was calculated as (LUC luminescence/rate of MUG accumulation ...
-
bioRxiv - Cell Biology 2020Quote: ... The proteins were then digested overnight at 37°C with 0.2 μg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on the column with 0.25 µg of Trypsin/Lys-C protease mix (Promega; cat. V5071), and peptides eluted for mass spectrometry.
-
bioRxiv - Microbiology 2019Quote: ... The membranes were washed with TBST and incubated with 200-fold diluted LgBiT protein in TBST (N2421, Promega), for 1 hour at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
-
bioRxiv - Cell Biology 2021Quote: The Rosa26 promoter and the 5’UTRs of secretory granule protein transcripts were subcloned into the pNL1.1 (Promega) using standard cloning techniques ...
-
ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... purified protein samples were prepared by S-alkylation with iodoacetamide and digestion with either with LysC/GluC (Promega) [RBD] or ...
-
bioRxiv - Biochemistry 2022Quote: ... laevis TRAIP that was produced in the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... The HaloTag-CpOGA proteins were mixed with equal molar of HaloTag Alexa Fluor 488 or 660 ligands (Promega) and incubated at room temperature for 30 min ...
-
bioRxiv - Physiology 2022Quote: ... Luciferase activities were measured and plotted relative to lysate protein concentrations using the Promega Dual Luciferase Reporter (Promega) assays according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
-
bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...