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Citations for Promega :
1151 - 1200 of 1768 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested using filter-aided sample preparation with sequence-grade trypsin (Promega, # V5111) at 37°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... Prior to tryptic digestion of the protein overnight using mass-spec grade Trypsin (Promega), protein was reduced and alkylated using dithiothreitol and iodoacetamide ...
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bioRxiv - Physiology 2024Quote: ... Chemiluminescent detection of in vitro translated protein was performed following the manufacturer’s protocol (Promega).
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37 °C with LysC (Wako Chemicals) and trypsin (Promega) in a 1/100 enzyme-to-substrate ratio ...
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bioRxiv - Cell Biology 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining volume in the tube (40 µL) was purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) following the manufacturers’ protocol ...
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bioRxiv - Genetics 2019Quote: ... the amplicons were purified from an agarose gel using the Wizard SV Genomic DNA Purification System (Promega Corp., Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... The amplified DNA from each potential off-target site was purified using the Wizard ® SV Gel and PCR Clean-Up System (Promega). The concentration of the purified DNA from the potential off-target sites and the template DNA from the positive control was assessed by using a Nanodrop 2000 Spectrophotometer (Thermo-Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... 3ul of gel extracted PCR product was used for TA cloning with the pGEM®-T Easy Vector System (Promega, A1360) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... were pooled and library cleanup was performed using a Wizard SV Gel and PCR Clean-Up System A9282 (Promega, Madison, WI). The pooled library was submitted to the UW Madison Biotechnology Center (UW-Madison ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega), ligated and subsequently cloned into E ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were directly sequenced using PCR primers after purification with Gel Clean-up System (Promega Corporation, Madison, WI, USA). All sequencing reactions were done on ABI 3700 DNA automated sequencer with the BigDye chemistry (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Amplified fragment isolated by agarose gel band extraction after electrophoresis and cloned into a pGemT easy vector described by the manufacturer (Promega, Germany). Isolated plasmid DNA was sequenced to confirm the identity and orientation of each clone.
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bioRxiv - Neuroscience 2020Quote: ... and bands of expected length were excised and purified using the Wizard® SV Gel and PCR clean-up system (Promega). Sequencing PCR was performed using the purified PCR products ...
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bioRxiv - Neuroscience 2021Quote: ... The 145-160 bp bands (which correspond to inserts of 24-32 nt cDNAs) were extracted and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). The quality of the library was assessed by the Experion DNA 1K chips (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al ...
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bioRxiv - Immunology 2022Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to manufacturer’s instructions and quantified by Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina MiSeq PE300 platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial counts were obtained by comparing the signals from the tested samples with a standard curve prepared by serial dilutions of a standard sample purified using the WizardSV Gel and PCR Clean-Up System Kit (Promega, USA) and subsequently cloned into the pGEM-T vector (Promega ...
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bioRxiv - Microbiology 2019Quote: ... were pooled and library cleanup was performed using a Wizard SV Gel and PCR Clean-Up System A9282 (Promega, Madison, WI). The pooled library was submitted to the UW Madison Biotechnology Center (UW-Madison ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR amplicon from the second-round PCR was gel-purified and ligated to the pGEMT- Easy vector (Promega, USA), as per the manufacturer-recommended protocol (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... after which fragments between 300-350 base pairs (bp) were excised and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). The size selected library was diluted to 12 pM and sequencing was performed using a 600 cycle (paired-end ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were purified using a HiPure Gel Pure DNA Mini Kit (Magen, D2111) and cloned into the pGEM-T Easy Vector (Promega, A1360). For each sample ...
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bioRxiv - Microbiology 2019Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to manufacturer’s instructions and quantified using Quantus™ Fluorometer (Promega, USA). The qualified PCR products were mixed ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified PCR products were purified on 1.5% agarose gels and cloned into the pGEM-T vector (Promega Corporation, Madison, WI) for sequence analysis ...
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bioRxiv - Cell Biology 2021Quote: ... YFP-NLS plasmid was digested by BamHI and HindIII and purified by Wizard® SV Gel and PCR Clean-Up System (Promega). Annealed oligonucleotides encoding UPF1’s NES with HindIII and BamHI overhangs at the end (UPF1-NES wt ...
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bioRxiv - Cell Biology 2021Quote: ... were generated by digesting UPF1-GFP iso2 plasmid with BsrGI and XbaI (both sites located at the very end of GFP) and purifying it by Wizard® SV Gel and PCR Clean-Up System (Promega). Annealed oligonucleotides encoding the NLS of SV40 or NES of snurportin-1 with a stop codon and BsrGI and XbaI overhangs at the end are ligated into the linearised UPF1-GFP iso2 vector (oligonucleotides listed in Table S1) ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were dried with 200 μl of ACN and digested with 10 ng/μl Lys-C (sequencing grade, Promega, USA) in 50 mM NH4HCO3 overnight at 37 °C (14).
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bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Bioengineering 2024Quote: The viability of human fibroblasts embedded in the bioprinted gel constructs was analyzed by monitoring the metabolic activity through the fluorimetric resazurin reduction method (CellTiter-Blue, Promega, G8080) at 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... whereafter the resulting DNA fragments were separated by gel electrophoresis and the required 5821 bp band was purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). In this linear form ...
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bioRxiv - Cancer Biology 2023Quote: ... Assessment of DNA fragment length was performed by electrophoretic separation in a 0.4% agarose gel with DNA ladder (Promega, WI, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel slices were then dried again and treated with an ice-cold solution of 12.5 ng/μL trypsin (Promega, Madison, WI) in 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified ORFs were then used as templates in a PCR reaction to amplify a full-length mutant amplicon followed by their purification using Wizard SV Gel and PCR Clean-Up kit (Promega, USA). These ORFs were digested with KpnI and BamHI enzymes and ligated into similarly digested pDsRed vector ...
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bioRxiv - Plant Biology 2024Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... the gel was transferred to a PVDF membrane and western blotting was performed using an anti-Halo primary antibody (Promega, #G9211) and a goat anti-mouse secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... were pooled and library cleanup was performed using a Wizard SV Gel and PCR Clean- Up System A9282 (Promega, Madison, WI). The pooled library was submitted to the UW Madison Biotechnology Center (UW-Madison ...
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bioRxiv - Genetics 2020Quote: ... The appropriate band (1595 bp) was excised and purified as recommended by the Wizard SV Gel and PCR Clean-Up System (Promega, Fitchburg, USA). The blunt end PCR product was A-tailed with a 20 min incubation step at 72 °C by adding 0.2 mM dATP ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was reduced by 10mM DTT in 100mM Ambic for 30 min and alkylated with 55mM iodoacetamide in 100mM Ambic for 20 min followed by in-gel digestion with 0.3 mg chymotrypsin (modified, Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate for 5 h at 40°C ...