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Citations for Promega :
1051 - 1100 of 1768 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Bands purification were made with Wizard® SV Gel and PCR Clean-Up System (Promega, 2800 Woods Hollow Road·Madison, USA). Next ...
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bioRxiv - Genetics 2021Quote: ... 3ul of gel extracted PCR product was used for TA cloning with the pGEM-T Easy Vector System (Promega, A1360) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA), quantified ...
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments containing the T7 promoter sequence were further amplified by PCR and purified using a Wizard SV Gel and PCR clean-up system (#A9281, Promega) to prepare templates for in vitro transcription ...
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bioRxiv - Biochemistry 2022Quote: ... Gel pieces were rehydrated with 50 mM ammonium bicarbonate containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The gel pieces of the different samples were washed and digested over night at 37 °C with MS-grade trypsin (Promega). MS/MS analysis was done using a previously described setup of a Waters nanoACQUITY gradient UPLC (www.waters.com ...
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bioRxiv - Neuroscience 2022Quote: ... Digestion of proteins in the gel pieces was done by covering them with a trypsin solution containing (8 ng/µL sequencing-grade trypsin (Promega), dissolved in 50 mM NH4HCO3with 10% ACN) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified on a 1% agarose gel and ligated into a pGEM-T Easy Vector Systems (Promega) plasmid followed by transformation in E ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were visualized using agarose gel electrophoresis and subsequently cloned into a pGEM-T easy cloning vector (Promega, Australia). Blue/white colonies were screened using standard techniques and a test digestion with endonuclease restriction enzyme EcoR1 was carried out to confirm insertion ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplicons were separated by electrophoresis and the corresponding bands were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) kit ...
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bioRxiv - Biochemistry 2023Quote: The gel pieces were then incubated with 60 µL of a 50 mM ammonium bicarbonate solution of trypsin (MS grade, Promega) at 4°C for 90 minutes and incubated at 37°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... ∼1/3 of the Coomassie-stained band was cleaved from the gel and samples were prepared with Trypsin Gold (Promega) in accordance with manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The gel lanes corresponding to pulsed-SILAC biological triplicates were cut alike into five bands and subjected to a manual in-gel digestion with modified porcine trypsin (Trypsin Gold, Promega). After destaining ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were dehydrated using 100% ACN before 25 µl of 12 ng/µl sequence grade modified trypsin porcine (Promega) in 25 mM ABC was added ...
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Molecular Biology 2023Quote: ... Reduction, alkylation and in-gel digestion were performed as described (Shevchenko et al., 2006) except that Trypsin/LysC mix (Promega) was used instead of trypsin ...
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bioRxiv - Biochemistry 2023Quote: ... Northern probes were generated from gel-purified PCR products corresponding to the RNAi inserts and radioactively labelled using the Prime-a-Gene labelling system (Promega).
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bioRxiv - Biochemistry 2022Quote: ... PCR products with desired length were gel purified and cloned into the MluI and NotI sites of mammalian expression vector pCI (Promega) that contains rho-tag ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified DNA fragments were purified using Wizard SV Gel and the PCR Clean-Up System (Cat. N° A9281 Promega), then the DNA was sequenced using the Macrogen facility (https://dna.macrogen.com/).
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bioRxiv - Cell Biology 2023Quote: ... The gel slices were then reduced and alkylated followed by destaining and in-gel digestion using 125 ng Trypsin/LysC (V5072, Promega) as previously described (Shevchenko ...
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bioRxiv - Biochemistry 2023Quote: ... Selected gel bands that did not bind to the 4’-methylgenistein beads were subjected to in-gel digestion with trypsin (MS grade; Promega). Peptide fragments were analyzed using a Q-TOF/MS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... gel pieces were dried and subsequently rehydrated in solution containing 12 ng/μL Trypsin Gold (mass spectrometry grade from Promega), 0.01% ProteaseMAX surfactant (Promega ...
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bioRxiv - Synthetic Biology 2023Quote: ... while the purification of the PCR fragments was performed using the Wizard SV Gel and PCR Clean-Up System (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... recombinant methylated BCAR3 (see methylation assay method) was sliced from the gel and digested with modified trypsin (sequencing grade, Promega). The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Systems Biology 2023Quote: ... and the bands at the predicted product size were excised and purified with a gel and PCR clean-up system (Promega). Sanger sequencing was completed by the Micromon facility at Monash University ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products with desired length were gel purified and cloned into the MluI and NotI sites of the mammalian expression vector pCI (Promega) that contains rho-tag ...
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bioRxiv - Microbiology 2024Quote: ... Lanes containing proteins were excised in two bands and subjected to a manual in-gel digestion with modified porcine trypsin (Trypsin Gold, Promega). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... then resuspended in 1 gel volume of 25 mM ABC containing 5 ng/µL of digestion grade trypsin (Promega, V5111) and incubated at 37°C for 16 hours for a full digestion or for 15 minutes for a partial digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... The bands of interest were cut from the Coomassie stained gel using the western blot filter as a guide and an in-gel digestion using a protease (Sequencing grade Trypsin, Promega) was performed prior to proteomic analysis by nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified through an 0.8% agarose gel, recovered (ZymoResearch, USA) and ligated into pGEM-T (Promega, USA). The ligation reaction was transformed into DH5α Escherichia coli (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
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bioRxiv - Synthetic Biology 2024Quote: ... Strings and plasmid backbones were amplified by PCR followed by purification with the Wizard SV Gel and PCR Clean-up System (Promega). Plasmids were assembled using isothermal GibsonAssembly® reaction (NEBuilder® HiFi DNA Assembly Master Mix).
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bioRxiv - Plant Biology 2019Quote: ... In the next step proteins were digested overnight using 100 ng/µl trypsin (Promega) at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins bound to streptavidin-sepharose matrix were digested with trypsin (Promega, Madison, WI, USA) during 16 h or eluted for western blot analysis ...
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bioRxiv - Genetics 2021Quote: The in vitro protein translations were performed using the TNT-assay (L4610) from Promega according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Tryptic digestion of proteins was carried out using porcine trypsin (Promega, GmbH, Mannheim, Germany). Trypsin solution prepared in 100 mM Ambic and 1 mM CaCl2 at a 0.01 μg/μl concentration and 100 μl added onto each sample for a final concentration of ~1.2 μg trypsin per sample containing 30 μg of total protein (trypsin to sample ration 1:25) ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested in 1 M urea with modified trypsin (Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) overnight and trypsin (Trypsin Gold, Promega) for 6 hours.
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bioRxiv - Molecular Biology 2020Quote: ... Protein pellets were resuspended in 8 M urea buffer combined with protease inhibitor (Promega) and purified using the chloroform-methanol precipitation method (Wessel & Flügge ...
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bioRxiv - Biophysics 2022Quote: ... The purified Kif5B protein was incubated with 10 µM HaloTag PEG-Biotin ligand (Promega) for 30 min on ice to produce a final construct of Kif5B homodimer with two C-terminal biotin tags ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Cancer Biology 2019Quote: Proteins were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation methods[13] ...
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bioRxiv - Immunology 2020Quote: ... IgG was purified from culture supernatant using Magne Protein A beads (Promega, Madison, WI) and the elution buffer exchanged with PBS using Amicon Ultra centrifugal filters (Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... samples containing 100 μg total protein were mixed with 100 μl Luciferase Substrate (Promega). The kinetics of the luminescence was recorded for 10 min using a TriStar2 S LB 942 Plate Reader (Berthold Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were digested overnight at 37°C with Sequencing Grade Modified Trypsin (Promega) and the reaction was stopped by acidification ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 3 μg trypsin (Promega) and 2 μg LysC (Promega) ...
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bioRxiv - Cell Biology 2021Quote: Proteins were synthesized using TNT® SP6 Quick Coupled Transcription/Translation system (Promega L2080) using the standard reaction mix (rabbit reticulocyte lysate plus amino acids ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...