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Citations for Promega :
1151 - 1200 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Wizard PCR Preps DNA Purification System (Promega) and DNA sequencing was performed using primer 2569 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Both PCR products were purified using the Wizard PCR cleanup kit (Promega, USA).
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bioRxiv - Zoology 2021Quote: ... PCR products were purified with Wizard SV Gel and PCR Cleanup kit (Promega). Purified products were sequenced at Sequetech DNA Sequence Service (Mountain View ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the GoTaq polymerase (Promega) and a StepOnePlus real-time PCR device (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... and qRT-PCR was performed using the GoTaq qRT-PCR master mix (Promega) with the ViiA7 Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega, M0482S) with qTOWER3 (Analytik Jena) ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were carried out using a 2x GoTaq PCR master mix (Promega), biotin-labeled M13F and biotin-labeled M13R primers ...
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bioRxiv - Genomics 2024Quote: ... The PCR product was cleaned using a PCR clean-up kit (Promega, A9281). The PCR product was then cloned into a lentiviral vector using Golden Gate cloning (Benchling ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR products were purified using Wizard Gel and PCR Cleanup System (Promega). Purified amplicons were diluted and pooled in equimolar concentrations ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CellTiter 96 Aqueous One Solution Proliferation Solution for 1 hour (Promega, Fitchburg, WI). Absorbance of the solution was then read at 490 nm ...
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bioRxiv - Synthetic Biology 2024Quote: ... pre-ran reaction solutions were incubated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by tC18 SepPak (50 mg ...
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bioRxiv - Microbiology 2020Quote: ... ratio 50:1 (protein:rLysC) for 3h/37°C and then digested with Sequencing Grade Modified Trypsin (#V5111, Promega, France) ratio 50:1 (protein:trypsin ...
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bioRxiv - Cell Biology 2020Quote: ... the gel pieces were covered with 200 μl of 20 ng/μl of sequence grade trypsin/Lys-C (Promega) in 50 mM ammonium bicarbonate pH8 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin digestion was performed by addition of 30 µL of 10 ng/µL sequencing grade trypsin (Promega, Madison, WI) in AmBic followed by 30 µL of additional AmBic ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: Proteins were digested overnight at 37°C in 20 ml of 25 mM NH4HCO3 containing sequencing-grade trypsin (12.5 mg/ml; Promega). The resulting peptides were sequentially extracted with 70% acetonitrile and 0.1% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fujifilm) and then incubated for 8 h at 37 °C with sequence-grade trypsin (1□g; Promega, Madison, Wisconsin). Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Digested proteins were finally desalted using a C18 TopTip (PolyLC ...
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bioRxiv - Cell Biology 2020Quote: Fractions corresponding to selected chromatographic peaks were treated with RNase A and later with sequencing grade modified Trypsin (Promega). Peptides were then purified using a C18 ZipTip (Merck Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... The solution was diluted to below 1M Urea using 200mM bicarbonate buffer and an aliquot of approximately 25μg protein were digested using sequencing grade Trypsin (V511A, Promega) at 37°C over-night (Trypsin to protein ratio of approx ...
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bioRxiv - Cancer Biology 2020Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 0.2 mg of protein was subjected to overnight digestion with 8.0 µg of mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 200 µl of 25 mM ABC containing 500 ng of sequencing grade trypsin (Promega, catalog # V5111) and incubated for 16 h at 37°C ...
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bioRxiv - Systems Biology 2021Quote: ... alkylated in the dark with iodoacetamide and applied to S-Traps (micro; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were further diluted to 0.5 M GuHCl with 10 mM EPPS pH 8.0 and digested with an additional 10 ng/μL LysC and 20 ng/μL sequencing grade Trypsin (Promega) at 37 °C for 16 hours.
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bioRxiv - Microbiology 2021Quote: ... Protein samples were precipitated with 0.1 M ammonium acetate in 100% methanol and the protein pellets were further digested with sequencing-grade trypsin (Promega). For the analysis involving the rpsAΔ6 mutant missing the last C-terminal OB fold domain of protein S1 ...
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bioRxiv - Microbiology 2020Quote: ... After washing with 150 μL of binding buffer four times the samples were subjected to proteolytic digestion using 1.2 μg trypsin (sequencing grade, Promega) for 2h at 47°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and the sample was digested at 37 °C with sequencing-grade trypsin (Promega; 1:50 protease:protein w/w ratio) or at 25°C with Glu-C (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... alkylated using 50 mM iodoacetamide for 2 hrs at ambient temperature and then digested with 1.2 μg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol (27) ...
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bioRxiv - Cancer Biology 2020Quote: ... alkylated in the dark with iodoacetamide and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of guanidium hydrochloride was reduced to 0.5 M by dilution with 100 mM ammonium bicarbonate and the samples were digested with 0.5 μg sequencing grade trypsin (Promega) over-night at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... Trypsin digested peptides were diluted to 200 μL with final concentration of 100 mM NH4HCO3 and 0.02% ProteaseMAX Surfactant prior to the addition of sequencing grade chymotrypsin (Promega) to a final 1:20 protease:protein ratio (w:w) ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10 mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by Oasis Prime HLB columns (30 mg ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then diluted with water (1:5) and incubated overnight at 37°C with 0.5 µg of sequencing grade modified trypsin (Promega). Samples were acidified using 1% TFA and peptides separated into 3 fractions on a 200 µL StageTips packed with three Empore™ SPE Disks SDB-RPS (3M ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were diluted to a final concentration of 2 M urea by addition of 100 mM NH4OAC (pH 6.8) and were digested overnight with sequencing-grade trypsin (Promega) at a 1:100 (enzyme:substrate ...
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bioRxiv - Plant Biology 2022Quote: ... Samples were diluted with 900 µl of 25 mM ammonium bicarbonate and digested overnight at 37°C in the presence of 0.35 µg of sequencing grade modified porcine trypsin (Promega). Peptides were vacuum-dried in a centrifugal evaporator to approximately 250 µl ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were further diluted to 0.5 M GuaCl with 10 M EPPS pH 8.5 and digested with an additional 10 ng/µL LysC and 20 ng/µL sequencing grade Trypsin (Promega) at 37°C for 16 h ...
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at 30° C in the dark and finally tryptically digested with 1.25 µg of sequencing grade porcine trypsin (Promega) in 100 mM ammonium bicarbonate at 37° C for 16 h ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant used for filter-aided sample preparation as described elsewhere [46] using 0.5 μg/sample of trypsin (sequencing grade, Promega). Resulting peptides were used for LC-MS/MS analyses ...
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bioRxiv - Microbiology 2020Quote: ... The proteins in the sample were digested with 10 μg/mL sequence grade modified trypsin (Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Proteolytic digestion was performed overnight at 37°C using 0.5 μg Trypsin/Lys-C Mix Mass Spectrometry Grade for each sample (Promega Corporation ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Immunology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately O/N using trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Neuroscience 2020Quote: A total of 100 μg protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation methods [19] ...
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bioRxiv - Molecular Biology 2022Quote: ... A second digestion was performed o/n at 37°C while shaking at 1,400 rpm using 500 ng of sequencing grade modified trypsin (Promega). Trypsin digested peptides were separated from Ni-NTA beads by filtering through a 0.45 µm filter Ultrafree-MC-HV spin column (Merck-Millipore).
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bioRxiv - Genetics 2022Quote: ... Protein-bound beads were washed in 80% ethanol and proteins were digested off the beads by addition of 0.8ug sequencing grade modified trypsin (Promega) in 100mM Ammonium bicarbonate ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were precipitated overnight with 5 volumes of cold 0.1 M ammonium acetate in 100% methanol and digested with sequencing-grade trypsin (Promega) and each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then diluted 50% with PBS before sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) was added for an overnight incubation at 37 °C ...