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Citations for Promega :
951 - 1000 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50 w/w; Promega,V5113) for 12 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... In-gel digestion was performed using 5 ng/μL mass spectrometry-grade trypsin (Trypsin Gold, Promega) in 50 mM NH4HCO3 digestion buffer ...
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bioRxiv - Plant Biology 2020Quote: ... and digested overnight at 37°C with 0.5 µg of sequencing-grade modified porcine trypsin (Promega). The resulting peptides were lyophilized to a final volume of ∼250 µl ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade trypsin and LysC (Promega). The resulting peptides were labeled using a tandem mass tag 10-plex isobaric label reagent set (Thermo ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Faecal material was removed by aspiration with molecular grade H20 (∼50 μl) and extracted by Promega Wizard® purification (see SI) ...
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bioRxiv - Biochemistry 2021Quote: ... followed by adding sequencing grade Trypsin at the ratio of 1:50 (Promega, cat. no. V5111) for digestion at 37 °C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Trypsin digestion was done using sequencing-grade modified Trypsin (0.4 μg/sample; Promega Corp., Madison, WI) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... On-bead tryptic digestion was performed with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammonium bicarbonate pH 8.3 overnight at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... mixed 1 to 1 (v / v) with 10 μg / ml trypsin (Sequencing Grade Modified Trypsin, Promega) in 50 mM ammonium bicarbonate and incubated for 2 hours at 60 °C for enzymatic hydrolysis ...
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bioRxiv - Cell Biology 2022Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were then separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Microbiology 2022Quote: ... reduced using DTT and alkylated using iodoacetamide and digested using sequencing grade Trypsin (Promega, Madison, WI). Aliquots of ca ...
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bioRxiv - Microbiology 2022Quote: ... Protein was then digested with 0.5 µg Sequencing Grade Modified Trypsin (V5111 - Promega, Madison, Wisconsin, USA). Following the overnight digestion at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were labeled using tandem mass tag isobaric labeling reagents (Thermo ...
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bioRxiv - Plant Biology 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using either sequencing-grade trypsin (Promega) or EndoGluC (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were labeled using tandem mass tag isobaric labeling reagents (Thermo ...
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bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
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bioRxiv - Neuroscience 2024Quote: ... In-gel digestion was performed overnight at 37 °C with MS grade endo Trypsin/LysC (Promega). The digested peptides were dried using a speedvac and stored at − 20 °C until further processed ...
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bioRxiv - Microbiology 2024Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Peptides were separated on an Acquity BEH C18 column (100 x 1.0 mm ...
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bioRxiv - Genetics 2024Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were then separated by reverse phase XSelect CSH C18 2.5 um resin (Waters ...
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bioRxiv - Biochemistry 2023Quote: ... After digestion overnight with sequencing grade porcine trypsin (300 ng, w/w, Promega, Fitchburg, MA, USA), the generated peptides were analyzed either using Easy-nanoLC-1000 system coupled to a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... and digested on-bead using filter-aided sample preparation with sequencing-grade modified porcine trypsin (Promega) (106) ...
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bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
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bioRxiv - Biochemistry 2023Quote: ... A mixture of trypsin and Endo-Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega) was used for digestion of the sample ...
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bioRxiv - Biochemistry 2024Quote: ... 90 µL of 25 mM ammonium bicarbonate containing 0.5 µg of MS grade Trypsin/LysC (Promega) (enzyme/protein ratio of 1:20 (w/w) ...
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bioRxiv - Biochemistry 2024Quote: ... in-gel digestion using 10 ng/µL of sequencing grade modified trypsin (Promega, Madison, WI, USA) was performed overnight at 37 °C.
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bioRxiv - Biophysics 2024Quote: ... gel plugs were crushed into small pieces and 5–10 μg of sequencing-grade trypsin (Promega) per ∼100 μg protein were added ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were digested via addition of two aliquots of sequencing-grade trypsin (Promega, 1:50 (w:w)) at two different sample dilutions ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at 37°C with 100 ng trypsin (sequence grade, Promega). The peptides formed from the digestion were further purified by µC18 ZipTips (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... and sequencing grade trypsin (10 μL, 0.5 mg/mL in 50 mM ammonium bicarbonate, Promega, V5111) were added for overnight incubation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and 125 μL digestion buffer (50 mM triethylammonium bicarbonate; TEAB) supplemented with sequencing grade Trypsin (Promega) at a concentration of 1 μg trypsin ...
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bioRxiv - Plant Biology 2023Quote: ... the gels were soaked with 50 mM TEAB containing 10 ng/µl Sequencing Grade Trypsin (Promega) and incubated at 40 °C for 8 h ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were digested overnight with LysC (FUJIFILM Wako) and trypsin (sequence grade; Promega, Madison, WI) at the protein:enzyme ratio of 100:1 for each enzyme at 25 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Tryptic digestion was performed by adding 1.6 µg of trypsin (V5111, Promega, sequencing grade modified trypsin) in 40 µl 10% ACN in 50 mM TEAB and incubated at 37 °C under humid conditions ...
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bioRxiv - Cell Biology 2023Quote: ... For Non-SILAC samples, 1 µl trypsin (1 µg/µl Trypsin Gold, Mass Spectrometry Grade (Promega)) was added and samples were incubated at 37°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested atop the filters overnight at 37°C with mass spectrometry grade trypsin (Promega) at a ratio of 1:50 enzyme:protein ...
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bioRxiv - Cancer Biology 2024Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were labeled using a tandem mass tag 10-plex isobaric label reagent set (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were digested atop the filters overnight at 37°C with mass spectrometry grade trypsin (Promega) at a ratio of 1:50 enzyme:protein ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were alkylated in-gel prior to digestion with 100 ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Microbiology 2024Quote: ... For digestion, 100 mM ammonium bicarbonate (ABC, pH 8.0) containing sequencing-grade modified trypsin (Promega, #V5111) was added to the bead-bound proteins at a protein-to-enzyme ratio of 30:1 ...
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bioRxiv - Biochemistry 2024Quote: ... and purified by chloroform/methanol extraction prior to digestion with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were labeled using tandem mass tag isobaric labeling reagents (Thermo ...
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bioRxiv - Genetics 2024Quote: ... The samples were then digested using the sequencing grade trypsin/lys C rapid digestion kit (Promega) according to the manufacturer’s recommended protocol ...
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bioRxiv - Pathology 2021Quote: ... 5 μl MgCl2 solution (Promega), 5 μl of PCR DIG labelling mix (Roche) ...
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bioRxiv - Plant Biology 2022Quote: ... digested with trypsin solution (Promega), and peptide fingerprinting was performed with a MALDI-TOF MS mass spectrometer (AB SCIEX 5800 ...
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bioRxiv - Cancer Biology 2022Quote: ... Trypsin/Lys-C solution (Promega) was added and incubated at 37°C for 12 h ...
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bioRxiv - Biochemistry 2024Quote: ... A trypsin solution (Promega Inc.) was combined with 200 µL of digestion buffer and applied to the spin columns containing bound proteins followed by a 1 h incubation at 42°C ...
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bioRxiv - Developmental Biology 2021Quote: PCR (RT-PCR) was conducted using GoTaq DNA polymerase (Promega). PCR programs were the following ...
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table 3.