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Citations for Promega :
1051 - 1100 of 3646 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The beads were resuspended in 50 mM NH4HCO3 supplemented with trypsin (Mass Spectrometry Grade, Promega; Madison, US) at an enzyme-to-protein ratio of 1:25 (w/w ...
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bioRxiv - Immunology 2023Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg sequencing-grade trypsin (Promega) at 37°C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then digested first with 6.6μg of LysC at room temperature overnight followed with 1.65ug sequencing grade trypsin (Promega) for 6 hours at 47°C ...
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bioRxiv - Cell Biology 2023Quote: ... digested overnight at 37°C in the presence of 1.5 µg of sequencing grade modified trypsin (Promega). The resulting peptides were vacuum-dried in a vacuum concentrator to approximately 200 µL ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteolytic digestion was carried out with Trypsin/LysC Gold (Mass Spectrometry grade, Promega Corporation Cat No: V5072) at an enzyme to protein ratio of 1:40 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight (21 h) at 37 °C with 0.1 μg/μL trypsin (sequencing grade, Promega) dissolved in 1 mM HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... on-column trypsin digestion was performed by adding by mass spectrometry grade trypsin/Lys-C mix (Promega) in a 50 mM NH4HCO3 buffer overnight at 37 °C.
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 37°C with sequencing grade modified trypsin (0.01 mg/mL; Promega Corporation) to allow protein digestion ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM TCEP) was added to the sample followed by 40 µg of sequencing grade trypsin (Promega). Proteins were digested overnight rotating at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and in-gel digestion with sequencing grade modified trypsin (Promega, 12.5 ng/μL in 50 mM NH4HCO3). Samples were analyzed with a LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
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Nucleolar Pol II interactome reveals TBPL1, PAF1, and Pol I at intergenic rDNA drive rRNA biogenesisbioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed by incubating beads with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammbic (pH 8.3 ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies.
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight at room temperature with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Systems Biology 2024Quote: ... samples were diluted 1:4 with 50 mM Tris–HCl pH 8 and sequencing grade trypsin (Promega) was added at 1:50 ratio ...
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bioRxiv - Microbiology 2024Quote: ... Protein digestion occurred overnight at 37 °C and 300 rpm with 1.5 µg sequencing grade trypsin (Promega). 0.5 pmol of the PierceTM Peptide Retention Time Calibration mix (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37°C with 2 µg trypsin (Trypsin Gold, Mass spectrometry Grade, Promega). Digestion was stopped by adding 70 µl 1% trifluoroacetic acid (TFA ...
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bioRxiv - Systems Biology 2024Quote: ... Protein digestion was carried out overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI, USA) at 1:40 (w/w ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestion buffer (50 mM TEAB pH 8.0) containing sequencing-grade modified trypsin (1/25, w/w; Promega) was added to the S-trap column and incubate for 1h at 47 °C ...
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bioRxiv - Cell Biology 2024Quote: ... One μg of mass spectrometry grade trypsin (Trypsin Gold, Promega; 1 mg/mL in 0.03% acetic acid) was added ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2022Quote: ... PCR was conducted in Promega PCR master mix (Promega, Madison, WI). SYBR green-based qPCR and probe-based qPCR was conducted in SsoAdvanced universal SYBR green supermix (Bio-Rad Canada ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified using PCR purification kit (Promega, WI, US), and ligated into PCR II Topo-vector (Invitrogen ...
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bioRxiv - Zoology 2024Quote: RT-PCR was performed using AccessQuick RT-PCR System (Promega, France) in total reaction volume of 25 µl with 5 µl of RNA extracts and 400 nM of forward primer (5’-ATTCGGGAGGAGACGTGGTA-3’ ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using GoTaq® Long PCR Master mix (Promega) and each reaction consisted of ...
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bioRxiv - Neuroscience 2023Quote: ... 100 microliters of CellTiter 96® AQueous One Solution Cell Proliferation Assay solution (MTS, Promega G3580) prepared in DMEM + 5% FBS was added to each well ...
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bioRxiv - Genomics 2024Quote: Cellular proliferation was assessed with the CellTiter 96 AQueous One Solution Cell Proliferation solution (Promega, G3582) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... 75 μL Nluc substrate solution (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... ECL solution was from Promega (W1001).
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bioRxiv - Plant Biology 2021Quote: ... 0.5% SDS (Promega 10% SDS solution), 0.2 M NaCl ...
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bioRxiv - Genomics 2024Quote: ... Celltiter-Glo solution (Promega, ref. G7573) was added to cells grown in 96-well plates ...
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bioRxiv - Physiology 2023Quote: ... Trypsin solution (25µg/mL, Promega France), 20 mM ammonium bicarbonate and 0.01 % glycerol (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: CellTiter-Glo 2.0 solution (Promega G9248) was used to measure the extracellular ATP released from spheroids ...
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bioRxiv - Cell Biology 2023Quote: ... 30 µL BrightGlo reagent solution (Promega) diluted 1:3 in water was added to each well and then ARE-LUC luminescence values were recorded on an Envision instrument (PerkinElmer).
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bioRxiv - Microbiology 2024Quote: ... 250µl of Protein Precipitation Solution (Promega) was added to the lysate ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was subjected to DpnI digestion and PCR cleanup (Promega Wizard SV Gel and PCR Clean-Up System). 30 ng of PCR amplified pUC19 ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM CaCl2 at pH 6.4) 0.2 µg/μL protein was mixed with proteases trypsin (Promega sequencing grade), chymotrypsin (BD) ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted with three volumes digestion buffer (100 mM Tris/HCl pH 8.5, 1 mM CaCl2) and 0.25 μg Trypsin (Sequencing Grade, Promega) were added and incubated o/n at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were digested by addition of 0.25 μg of sequencing grade trypsin dissolved in 50 mM acetic acid at 0.5 μg/mL (Promega) overnight at 37 °C with vigorous shaking ...
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bioRxiv - Biochemistry 2020Quote: ... Enzymatic digestion of was performed overnight at 37°C by the addition of sequencing grade trypsin (Promega, Wisconsin) (100 ng per sample ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... reduced (DTT) and alkylated (iodoacetamide) and subjected to enzymatic digestion with sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... 300 μL of 100 mM NH4HCO3 was added in to each sample with sequencing grade porcine trypsin (Promega) at a ratio of 1:20 overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Biochemistry 2022Quote: ... The denatured samples were diluted to 0.5% SDC and proteolysis was performed by the addition of 6,5 µg of sequencing grade trypsin (Promega) for 16 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were digested with shaking overnight at room temperature in 300 ng of sequencing grade modified trypsin (Promega). Samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was four-fold diluted and the protein digested in presence of 0.2 M Tris-HCl buffer pH 8.0 and 1 μg of sequencing grade trypsin (Promega) previously dissolved in 20 mM acetic acid ...
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bioRxiv - Neuroscience 2022Quote: ... then digested at a 1:25 enzyme:protein ratio with proteomics grade trypsin/rLysC (V5071; Promega, Madison, WI, USA) overnight at 37°C according to the SP3 method (Hughes et al ...
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bioRxiv - Molecular Biology 2021Quote: ... The gel sections were lyophilized again before in-gel trypsinization (20 mg/mL sequencing grade modified trypsin, Promega) overnight at 37°C with gentle shaking ...
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bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...