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Citations for Promega :
851 - 900 of 1665 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Treated proteins were then digested with sequencing grade modified trypsin (Promega, V5111) and incubated overnight (37 °C ...
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bioRxiv - Plant Biology 2023Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was further analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (1:50 enzyme: protein ratio; Promega, United States) at 37°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein-bead complexes were digested with a mixture of trypsin:LysC (Promega) at a 1:20 ratio overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... subjected to protein precipitation using Chloroform/Methanol and digested with trypsin (Promega). Desalted and acidified peptides (0.1% TFA ...
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bioRxiv - Cell Biology 2024Quote: ... before proteolytic digestion with LysC (enzyme to protein ratio 1:100, Promega) for 3 h and with trypsin (1:25 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were on-bead digested using 500 ng Sequencing Grade Trypsin (Promega). The digestion was carried out at 37°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... the gel pieces were covered with 200 μl of 20 ng/μl of sequence grade trypsin/Lys-C (Promega) in 50 mM ammonium bicarbonate pH8 buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and the final PCR product was gel-purified and subcloned into the pGEM-T Easy Vector (Promega, Guangzhou, China) for sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega) at 4°C before incubation overnight at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... The resulting amplicons were pooled by sample and purified using the Wizard SV-Gel and PCR Cleanup System (Promega). Samples were sequenced by the UW-Madison Biotechnology Center on an Illumina Hi-Seq 2500 in rapid mode ...
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bioRxiv - Bioengineering 2019Quote: ... Linearized plasmid DNA was purified using the Wizard® SV Gel and PCR Clean-up Kit (Promega, Madison, WI) in preparation for transfection into Jurkat cells.
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bioRxiv - Biophysics 2020Quote: ... Gel plugs were soaked in 50 mM ammonium bicarbonate (pH 7.8) containing 20 μg/mL of trypsin gold (Promega) for 24 hours at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... PCR reaction mixture was purified with the Wizard® SV Gel PCR Clean-Up System (Promega Corporation, WI, USA). One Shot® TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Tryptic in-gel digestion of gel slices was performed overnight at 37°C in 50 mM ammonium bicarbonate using 0.1 µg trypsin (Promega) per gel band ...
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bioRxiv - Microbiology 2019Quote: ... The 1400 bp long DNA fragments were purified (Wizard®SV Gel and PCR Clean-Up System, Promega, Germany) and sequenced using the same 27f and 1492r primer pair ...
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bioRxiv - Biochemistry 2019Quote: ... and the band corresponding to ~64 kDa was incised and in-gel digested by trypsin according to the manufacturer’s protocol (Promega). Peptides were then extracted from the gel and analyzed by LC-MS using an Eksigent nano-HPLC (model nanoLC-2D ...
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bioRxiv - Biochemistry 2020Quote: ... pKS was nicked with Nt.BbvCI and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Cat#A9281). The resulting short ssDNA fragment was then replaced with a 5-fluoro-2’-deoxycytidine (FdC)-modified oligonucleotide (400 nM ...
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bioRxiv - Microbiology 2020Quote: ... Protease was driven into the gel pieces by rehydrating them in 12 ng/ml trypsin in 0.01% ProteaseMAX Surfactant (Promega) for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... Enzymatic digestion of proteins was performed at 37°C overnight by incubating the gel pieces in 25 ng/mL pH 8.5 trypsin solution (Sequenced Grade Modified Trypsin, ref V511A, Porcine, Promega). Digested peptides were extracted by incubation in 70% and 100% acetonitrile for 20 min each ...
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bioRxiv - Biochemistry 2021Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
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bioRxiv - Neuroscience 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega) at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... due to the differentially spliced products) were run on agarose gels and cloned into pGemTeasy (Promega Corp, Wisconsin, USA). Different sized inserts were selected and sequenced.
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bioRxiv - Genetics 2022Quote: ... The corresponding bands were cut out and purified using wizard SV gel and PCR clean-up system (Promega, A9282). The concentrations of purified PCR products were measured by Nanodrop 2000c and then diluted into 70-100ng/uL with distilled water accordingly ...
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bioRxiv - Microbiology 2023Quote: ... The FAM-labeled probes were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and quantified with a NanoDrop 2000C (Thermo ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the WizardTM SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) following the manufactures instructions ...
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bioRxiv - Biochemistry 2023Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
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bioRxiv - Bioengineering 2023Quote: ... The obtained PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (A928, Promega) and blunt end ligation was performed followed by electroporation of freshly made competent cells [81] to yield 3.6×107 total transformants ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified after migration on a 1% agarose TAE 0.5X gel using the Wizard PCR Clean-Up system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was digested with ApaI and XhoI and then purified (Wizard® SV Gel, Promega, WI, USA). The eluted fragment was ligated downstream of an inducible acetoacetate decarboxylase promoter (Padc ...
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bioRxiv - Cell Biology 2024Quote: ... Purification and assembly of DNA fragments were performed using a Wizard SV Gel and PCR Clean-up System (Promega) and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... DNA were purified using the Wizard® SV Gel and PCR Clean-Up System protocol (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from PCR mixture was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was digested for overnight with 5 ng/µL trypsin (Promega Corporation) in 50 mM ammonium bicarbonate at 37 °C ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Developmental Biology 2020Quote: Proteins were digested on-bead with 0.5 ug Sequencing Grade Modified Trypsin (Promega) overnight at 37°C with agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA).
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bioRxiv - Neuroscience 2021Quote: ... The proteins were digested with 2.5 μg of sequencing grade modified trypsin (Promega). The resulting peptides purified with C18 MicroSpin columns (The Nest Group ...
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bioRxiv - Cell Biology 2019Quote: ... the bead-bound protein was incubated with either 6.7 µM HaloTag-AlexaFluor660 (Promega) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: ... proteins from HL-1 cells were extracted with Passive Lysis Buffer (Promega France). Quantification of bioluminescence was performed with a luminometer (Centro LB960 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested by adding sequencing grade trypsin (1:20 substrate:enzyme; Promega #5111) and incubating at 37°C for 16 h ...
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bioRxiv - Cell Biology 2020Quote: ... before analysis of luciferase protein levels using Dual-Glo luciferase assay system (Promega) and an EnSpire multimode plate reader (Perkin-Elmer).
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bioRxiv - Systems Biology 2020Quote: The total resultant protein content was digested with trypsin (Trypsin Gold, Promega, USA). The enzyme was added at the ratio of 1:40 (w/w ...