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Citations for Promega :
1001 - 1050 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR to detect ZIKV was performed in a 20 μL reaction final volume containing GoTaq one-step RT-qPCR master mix (Promega), 25 ng of sample RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
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The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in musclebioRxiv - Cell Biology 2022Quote: ... modified with 10mM Iodoacetamide in 100 mM ABC (at the dark, RT, 30 mins) and digested in 10% Acetonitrile and 10 mM ABC with modified trypsin (Promega) at a 1:10 enzyme-tosubstrate ratio overnight at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl of Reverse Transcription Mix (10 μl of 10 mM dNTPs and 40 μl of 5X MMLV RT Buffer Promega), 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Plant Biology 2020Quote: ... The concentrations of the resulting cDNAs were adjusted to 10 ng/µL and 2 µL were used for RT-qPCR reactions performed with the GoTaq qPCR Master Mix (Promega) in a final volume of 10 µL ...
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bioRxiv - Molecular Biology 2020Quote: ... Resulting ribosomal complexes were analyzed by toe-printing as described (Pisarev et al., 2007) using avian myeloblastosis virus reverse transcriptase (AMV RT) (Promega) and [32P]-labeled oligonucleotide primers complementary to HalV nt ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated at 37°C for 72 h after which they were lysed for 20 min on a shaker platform at RT by addition of Reporter Lysis Buffer (Promega). Luciferase signal was determined from cell lysates by adding the Bright-Glo Luciferase buffer (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
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bioRxiv - Developmental Biology 2019Quote: ... cells into micro-tubes containing 5 µl of cold 1X SuperScript IV VILO Master Mix for two-step RT-qPCR containing 6 units RNasin (Promega) and 0.5% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a DNase I treatment and a subsequent reverse transcription with oligo d[T]18 using the GoScript RT kit (Promega). One-step RT-qPCR was performed using the SYBR FAST Mix optimized for LightCycler 480 (KAPA ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA was synthetized from 500 ng total RNA by using random hexamers and moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) Point Mutant Synthesis System (Promega). Quantification was performed by using the LightCycler 480 Universal Probe Library System (Roche) ...
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bioRxiv - Immunology 2022Quote: ... followed by retrotranscription to cDNA with M-MLV RT, RNase H(–) point mutant (Promegain presence of buffer, nucleotides and RNasin Plus RNase Inhibitor (Promega). Reaction was then incubated at 40 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and alkylated with 5 mM of iodoacetamide 30 min in the dark at RT°C prior to digestion with 5 μg of trypsin Gold (Promega) for 15 h at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Biochemistry 2022Quote: ... RNA was isolated 24 hours after transfection and the pattern of alternative splicing was assessed by RT (with oligo-d(T) / random hexamer) and AMV Reverse Transcriptase (Promega) and PCR with GoTaq (Promega ...
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bioRxiv - Microbiology 2023Quote: ... and then reverse transcribed into complementary DNA (cDNA) using 30 enzyme units(U) of the MMLV-RT (Promega, WI, USA) and 1μM of the labeled primer ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with 2 μg of lysyl endopeptidase (Wako) at RT for overnight and were further digested overnight with 2 μg trypsin (Promega) at RT ...
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bioRxiv - Microbiology 2023Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were then incubated with blotting buffer containing 1:200 LgBiT for 1-2 h with rocking at RT (N2410, Promega). Next ...
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bioRxiv - Immunology 2023Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega, cat# A6110). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of each RNA sample was reverse-transcribed using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT, Promega, #M1701) and random hexamer primers (Thermo Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 µg of RNA was then used to synthesize cDNA in a 20 µl reverse transcription reaction using 5X First Strand Buffer and MMLV-RT (Promega) with random hexamers ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry and CLIP were PCR amplified from pFN21A (Promega), pSNAPf (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Genotypes were determined by PCR with GoTaq Green (Promega) or allelic discrimination for MommeD1 or MommeD43 ...
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bioRxiv - Cell Biology 2022Quote: ... Wizard SV Gel and PCR Clean-Up System (ProMega) was used on cDNA samples ...
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bioRxiv - Cancer Biology 2019Quote: ... and PCR reactions were performed using GoTaq Green (Promega) using 30 cycles of 94°C for 30 sec ...
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bioRxiv - Microbiology 2019Quote: ... and analysed by PCR with GoTaq Master Mix (Promega) using the following conditions ...
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bioRxiv - Microbiology 2019Quote: ... PCR was performed by using GoTaq Master Mixes (Promega) with the following conditions ...
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bioRxiv - Microbiology 2019Quote: ... 0.2 mM of each dNTP (PCR Nucleotide Mix, Promega), 0.1 μM of each primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative PCR was performed using SYBR-green mastermix (Promega) and run in a Prism 7900 HT instrument (Applied Biosystems) ...
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bioRxiv - Genetics 2020Quote: ... and 200 ng PCR product using FuGENE HD (Promega). At 2 days post transfection ...
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bioRxiv - Cancer Biology 2021Quote: Transcription templates were synthesized by PCR (GOTAQ G2, Promega) using a hybrid T7promoter/sequence-specific forward primer and a sequence-specific reverse primer ...
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bioRxiv - Cell Biology 2021Quote: ... PCR products were digested with SacI and KpnI (Promega) and ligated in the pZsGreen1-1 plasmid ...
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bioRxiv - Microbiology 2020Quote: PCRs were performed in 25 μl reaction buffer (Promega) with MgCl2 at 1.5mM final ...
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bioRxiv - Neuroscience 2020Quote: ... NanoLuc was PCR amplified from plasmid pNL.1.1 (Promega) and cloned into pUS2-MT to generate pUS2-MT-Nanoluc ...
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bioRxiv - Cell Biology 2020Quote: A GenePrint 10 System PCR Amplification kit (Promega Corporation) was used to determine the genetic signature of both samples based on the multiplex analysis of 9 loci and the Amelogenin sex-determining marker ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using GoTaq G2 DNA Polymerase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... were amplified by PCR from human genomic DNA (Promega), using the primers listed in Table S1 and then inserted into the psiCHECK-2 vector ...
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bioRxiv - Neuroscience 2019Quote: ... in 1 × PCR thermo DNA polymerase reaction buffer (Promega). Following the addition of 100 pMol each 5’ and 3’ primer and MgCl2 to 4 mM ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR products were cloned into pGEMT (Promega #1360) and sequenced to verify the mutations ...
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bioRxiv - Microbiology 2020Quote: ... sucrose-resistant colonies by PCR using GoTaq® (Promega) and primers listed in Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was subcloned into plasmid psiCHECK2 (Promega) previously digested with NotI using the In-Fusion cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using GoTaq Green master mix (Promega), per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR amplification was carried out using GoTaq polymerase (Promega) at 1.5 mM MgCl2 using the following cycling parameters ...
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bioRxiv - Genetics 2019Quote: ... A PCR reaction using GoTaq G2 DNA Polymerase (Promega) for Cep164tm1a(EUCOMM)Wtsi/+ ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were performed using Master Mix (2X) (Promega) and carried out at an initial denaturation step of 95 °C for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... PCR screens were carried out using GoTaq Green (Promega) with the primer combinations described.