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Citations for Promega :
801 - 850 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Colony PCR using GoTaq DNA polymerase (Promega) was performed on isolates not identified by MALDI-TOF MS ...
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bioRxiv - Molecular Biology 2023Quote: ... a PCR using GoTaq G2 polymerase (Promega) was performed to specifically amplify the wild-type target locus ...
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bioRxiv - Neuroscience 2023Quote: ... then PCR amplified with GoTaq MasterMix (Promega) and subsequently digested with a restriction enzyme to distinguish wild type and mutant alleles (Table 1) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCRs were performed with GoTaq Flexi (Promega) for 30 cycles of ...
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bioRxiv - Microbiology 2023Quote: PCR products and a standard ladder (Promega) were electrophoresed on 1% agarose gels in 1X TBE for 1 hr at 85V ...
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bioRxiv - Microbiology 2024Quote: ... 6μL Promega PCR amplification-grade water (Promega) and 2 μL of the sample DNA (corresponding to about 20 ng) ...
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bioRxiv - Microbiology 2019Quote: ... purified amplicons were inserted into plasmids and ligation reactions (10 μ1) were set up as per manufacturer’s instructions (Promega pGEM-T Easy vector system). After ligation ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
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bioRxiv - Cancer Biology 2020Quote: HPV16 integration into the IRF4 gene was confirmed by PCR using GoTaq Long PCR Master Mix kit (Promega U.S.), with 5’-AAGCATGTCAGACACGCAGA- 3’as a forward primer ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... PCR products were gel purified with either Wizard® SV Gel and PCR Clean-Up System (Promega cat# A9281) or Isolate II PCR and Gel Kit (Bioline cat# BIO-52059 ...
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bioRxiv - Biochemistry 2020Quote: ... PCR reaction mixture was purified with the Wizard® SV Gel PCR Clean-Up System (Promega Corporation, WI, USA). One Shot® TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
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bioRxiv - Physiology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control The relative level for each gene was calculated using the 2-DDCt method (Hulf et al. ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were purified using the WizardTM SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) following the manufactures instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The obtained cDNA was used as the template for quantitative real-time PCR (qRT-PCR) using qPCR Mastermix (Promega). mRNAs expression levels were normalized to actin-5C mRNA used as the internal control ...
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bioRxiv - Biochemistry 2023Quote: ... All PCR products were pooled and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). DNA (10 μg ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were gel-extracted using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison WI, USA) and sequenced by GENEWIZ (Leipzig ...
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bioRxiv - Bioengineering 2023Quote: ... The obtained PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (A928, Promega) and blunt end ligation was performed followed by electroporation of freshly made competent cells [81] to yield 3.6×107 total transformants ...
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from PCR mixture was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of total RNA was reverse transcribed using an oligo(dT)15 and the MMLV-RT (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Physiology 2021Quote: ... The resultant cDNA was assessed by RT-qPCR using SYBR® Green Supermix 2x qPCR master mix (Promega) in a ABI PRISM 7900HT Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: A total of 10 µg RNA was taken from each sample for reverse transcription (RT) using GoScript (Promega) reverse transcriptase (5 µg for RT ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the housekeeper gene Hprt (ID: Mm03024075_m1) using GoTaq Probe qPCR and RT-qPCR System (Promega, Madison, WI) and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis and qPCR were performed using the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) with an ABI Prism 7900HT Sequence Detection System (Applied Biosystems ...
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Identification of echinacoside as a tobramycin potentiator against Pseudomonas aeruginosa aggregatesbioRxiv - Microbiology 2024Quote: ... Such cDNA samples were applied as templates for RT-qPCR using 2× GoTaq® qPCR Master Mix (Promega). The reactions were performed on the CFX96TM system (BIO-RAD ...
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bioRxiv - Physiology 2024Quote: ... The resulting cDNA was analyzed by RT-qPCR using SYBR green fluorescent dye 2x qPCR master mix (Promega) in a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Microbiology 2019Quote: ... 500 nM of each primer (5’-TCCTGCTCAACTTCCTGTCGAG-3’ and 5’-CACAGGTCAAACCTCCTAGGAATG-3’) and 0.1 µL hot-start Taq DNA polymerase (Promega) in 20 mM Tris−HCl pH 8.3 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and cDNA synthesis was carried out using an oligo d(T)15 primer and MMLV reverse transcriptase (Promega, USA) enzyme ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Cell Biology 2019Quote: ... 500 ng RNA was used for reverse transcription using random primers and the GoScript master mix according to the manufacturer’s instructions (Promega). Quantitative real-time PCR was used to examine the expression levels of TRAP1 and HIF1A with GAPDH as the reference gene.
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bioRxiv - Molecular Biology 2019Quote: ... RNA from splicing reactions (∼20 fmol pre-mRNA in 10.5 µL splicing reaction) was analyzed by primer extension (15 µL reaction volume total) using AMV reverse transcriptase (Promega) as previously described (Mayas et al ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of forward and reverse primer (5 μM, see Supplementary Table 1) and 5.5 μL of Nuclease-free Water (Promega). The PCR I was performed as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription was performed on total RNA using the GoScript Reverse Transcription System with an oligo-dT primer (Promega). Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega ...
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bioRxiv - Plant Biology 2021Quote: ... 10 ng of RNA was used with oligo (dT) primers and AMV reverse transcriptase and reverse transcription was performed according to the manufacturer’s instructions (Promega). Using a GeneAmp 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h at 37 °C and cDNA was then synthesized using a Moloney murine leukemia virus (MMLV) reverse transcription kit with random primers following the manufactureŕs protocol (Promega). To check for the presence of remaining DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... 1μg of mRNA was reverse-transcribed using oligo-dT-VN primers and ImProm-II as the reverse transcriptase (Promega) in triplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 400 ng of RNA using ImProm-II Reverse Transcription system kit and random primers (Promega). Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 500 ng of RNA was added to each 11.8 ul reaction along with 0.5 ul of random primer (Promega C1181) and 1 ul of dNTP (Promega U1511) ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...
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bioRxiv - Immunology 2020Quote: ... was reverse transcribed from RNA extracts using GoScript reverse transcriptase with random primers according to the manufacturer’s instructions (Promega). qPCR reactions were carried out on a Stratagene Mx3005p thermal cycler (Agilent Technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... LaMATE2ORF sequence was cloned in pGEM-T easy vector (for primers see Table S1, Promega Italia Srl, Milan, Italy) and deposited in the NCBI database (KY464927).