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Citations for Promega :
851 - 900 of 2354 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed on total RNA using the GoScript Reverse Transcription System with an oligo-dT primer (Promega). Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vector using the manufacturer’s protocol (Promega; CAT #A1360). Vectors were transformed into E ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl (200 nM) of each primer and 10 μl of GoTaq® qPCR Mastermix 2X (Promega Corporation, USA). The amplification program consisted of ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized by reverse transcription using RevertedAid H minus reverse transcriptase (ThermoFischer Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3’ end of the MERS-CoV genome ...
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bioRxiv - Neuroscience 2021Quote: ... the extracted RNA was reverse-transcribed into cDNA with the oligo (dT)15 primer with GoScriptTM Reverse Transcriptase (Promega). In Fig ...
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bioRxiv - Molecular Biology 2022Quote: Edited loci were amplified using locus-specific primer pairs described in Supplementary Table 1 using GoTaq master mix (Promega) and 200ng of genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... [32P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random hexanucleotide-primer kit following the supplier’s instructions (Promega). As a loading control ...
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bioRxiv - Cell Biology 2023Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the ProNex Size- Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... Inserts (primers and size are presented in Supplementary Table S5) were cloned into pGEM-T Easy (Promega, Madison, WI) vector and confirmed by restriction and sequencing analysis ...
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bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Developmental Biology 2023Quote: ... A minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Molecular Biology 2024Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out with oligo(dT) primers using Moloney murine leukemia virus reverse transcriptase according to the manufacturer’s instructions (Promega). The qPCR reaction was performed on an Applied Biosystems QuantStudio3 thermocycler using a SYBR™ Green PCR Master Mix (Thermo Fisher scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA (1000ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript reverse transcriptase kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Cell Biology 2024Quote: ... reverse transcription was carried out using 1 μg of total RNA and random primers (Reverse Transcription System, Promega, A3500). IQTM SYBR® Green qPCR Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... The positive PCR products were cleaned using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI) and submitted for Sanger sequencing at Roy J ...
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bioRxiv - Genetics 2022Quote: Chromatin accessibility by real-time PCR (ChART-PCR) was performed as previously described (75) using 20 U DNase I (Promega). To assess nucleosome occupancy ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting cDNA was subjected to PCR or qPCR analysis using Phire Green Hot Start II PCR Master Mix (Promega) or GoTaq qPCR Master Mix (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Wisconsin, USA, A9281) and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes ...
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bioRxiv - Biochemistry 2021Quote: ... Both PCR products were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation [37] ...
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bioRxiv - Microbiology 2019Quote: ... Products of PCR reactions were purified using the Wizard® SV Gel and PCR Clean-Up system (Promega, Madison, USA). Sequencing reactions were performed using the Big-DyeTM Terminator Version 3.1 Ready Reaction Cycle Sequencing Kit (Applied BioSystems ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was amplified with Phusion HotStart II DNA polymerase and the PCR fragment was ligated with T4 ligase (Promega) and transformed into Novablue competent cells ...
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bioRxiv - Plant Biology 2020Quote: ... The overlapping PCR products were separated and purified by the Spin Column PCR Product Purification Kit (Wizard SV Gel and PCR Clean-Up System) following manufacturer’s instruction (Promega, WI). Then ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplicons were gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega Cat. No. A9281). Next ...
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bioRxiv - Genomics 2019Quote: ... PCR products run on 1% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up System (Promega). Purified PCR products were sequenced and analyzed with the Sequence Scanner Software 2 Version 2 (Applied Biosystems).
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA), quantified ...
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments containing the T7 promoter sequence were further amplified by PCR and purified using a Wizard SV Gel and PCR clean-up system (#A9281, Promega) to prepare templates for in vitro transcription ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative reverse transcription PCR (qRT-PCR) was performed according to the method of Ehira and Miyazaki43 using Go Taq qPCR Master Mix (Promega). Primers used for PCR and qRT-PCR are listed in Supplementary Table 5.
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Synthetic Biology 2023Quote: ... while the purification of the PCR fragments was performed using the Wizard SV Gel and PCR Clean-Up System (Promega).
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bioRxiv - Plant Biology 2023Quote: ... PCR reactions were conducted in 10 μL volume comprising 5 μL Promega 2x PCR Master Mix (Promega, Madison, Wisconsin, USA), 10 pmol of forward and reverse primers ...
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bioRxiv - Immunology 2021Quote: ... 400 µL PCR products were purified by Promega Wizard Gel/PCR purification columns ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel extracted (Promega, A9281). Wildtype embryos were injected with a mix containing two sgRNAs (56.25 ng/μl each) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 mM PCR dNTP mix (Promega) and 0.5 μl RNase free water ...
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bioRxiv - Zoology 2021Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Cell Biology 2021Quote: ... a PCR was done using Taq polymerase (Promega) and visualize on agarose gel to assess xbp1 ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed using Go Green Taq (Promega) according to the vendor protocol ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated to pGEM-T (Promega) or pBluescript SKII (− ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Colony PCR was performed using GoTaq polymerase (Promega). The ΔhilE hilD::mCherry was constructed by incorporating hilD::mCherry construct into the ΔhilE strain.
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bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
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bioRxiv - Microbiology 2022Quote: ... Diagnostic PCR was performed using GoTaq polymerase (Promega) or OneTaq DNA polymerase.
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed in GoTaq Master Mix (Promega) according to standard procedures ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed with GoTaq DNA polymerase (Promega); primer sequences are shown in supplemental Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
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bioRxiv - Zoology 2020Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 ul MgCl ...
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bioRxiv - Zoology 2020Quote: ... 10 μL 5× PCR buffer (Gotaq flexi, Promega), 8 μL 25mM MgCl ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were cloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with GoTaq Master Mix (Promega) with 1ul cDNA template at 1:20 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... and cleaned again using PCR cleanup (Promega, A6754). Plasmid DNA inputs were used in a set of concentrations ranging from 0.5 nM to 10 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... with mutagenesis performed by PCR using PfU (Promega). All mutagenesis was confirmed by Sanger Sequencing (Source Bioscience).