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Citations for Promega :
9851 - 9900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... the nitrite concentration in the supernatant was measured via the Griess assay (Promega, TB229), per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The performance of the LC-MS system was controlled by analyzing a yeast protein extract digest (Promega). A total of eight datasets were collected and subsequently processed using three de novo sequencing algorithms CasaNova ...
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bioRxiv - Biochemistry 2023Quote: Glycosyltransferase activity was assayed using a UDP-GloTM Glycosyltransferase Assay kit (Promega, V6961). A 25 μL reaction was initiated by adding 50 nM and 100 nM of purified wild type and mutant T.gondii-GalNAc-T3 ...
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bioRxiv - Biochemistry 2023Quote: Cell viability was evaluated by measuring ATP levels using CellTiter Glo (Promega Cat.n. G7570) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... We employed a two-step digestion process (involving lysyl endopeptidase, from FUJIFILM Wako Chemicals U.S.A. Corporation, followed by trypsin, Promega) in-solution to produce the crosslinked peptide pairs.
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bioRxiv - Biochemistry 2023Quote: ... Trypsin (Promega, Madison, WI, USA) was added at a 1:25 (v/v ...
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bioRxiv - Biochemistry 2023Quote: To assess any toxicity of inhibitors LDH release was assessed using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with Diamond stain (Promega) and imaged on a Bio-Rad ChemiDoc MP.
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bioRxiv - Biochemistry 2023Quote: ... alkylated with 55 mM iodoacetamide for 20min at room temperature and digested using 13 ng/μl trypsin (Promega) overnight at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... in a GloMax® Discover Microplate Reader (Promega, UK). Results expressed as nanogram (ng ...
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bioRxiv - Biochemistry 2023Quote: ... using the FuGENE®6 transfection reagent (Promega), and virus particles were harvested after 7 d of infection ...
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bioRxiv - Biochemistry 2023Quote: The FASP method[7] was used to digest urine protein with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA). One hundred micrograms of urine protein was added in the membrane of a 10KD ultrafiltration tube (Pall ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Biochemistry 2023Quote: ... Treated proteins were then digested with sequencing grade modified trypsin (Promega, V5111) and incubated overnight (37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 4 M urea containing 0.1 % Protease Max (Promega Corp. V2071) and diluted in 40 mM ammonium bicarbonate buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated at 37°C for 24 h before proliferation was assayed by Hoechst staining or WST-1 and luciferase signal was measured by Steady-Glo® Luciferase Assay System (Promega, E2520).
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bioRxiv - Biochemistry 2023Quote: ... beads were resuspended in 100 μL of 50 mM TEAB and enzymatically digested overnight using sequencing-grade trypsin (Promega, V5111). Digested peptides were eluted through centrifugation and labeled using commercially available TMTsixplex tags (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were transfected into HEK293T cells using FuGENE HD (Promega, E2312), and proteins were allowed to express for 20 h ...
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bioRxiv - Biochemistry 2023Quote: BRET substrate (Nano-Glo®) was purchased from Promega. DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was measured daily using the MTS assay (CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay; Promega, G5430) across 3 d (days 1 – 3) ...
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bioRxiv - Biochemistry 2023Quote: ... and Trypsin (#V5111) were obtained from Promega. Cysteine protease IgdE (FabALACTICA ...
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bioRxiv - Biochemistry 2023Quote: ... The captured proteins were washed twice in 200μl 95% MeCN and transferred to 200μl 25mM ammonium bicarbonate containing 1 μg sequencing grade modified trypsin (Promega, Madison, USA) and mixed for 4 h at 37°C ...
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Balanced Interplay Between Hsp110, Hsp70 and Class B J-Domain Protein Improves Aggregate DisassemblybioRxiv - Biochemistry 2023Quote: ... Untagged luciferase was purchased from Promega (E1702).
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase activity was quantified with a Luciferase detection kit (Promega, Madison, USA) according to the manufacturer’s instructions using a Sirius single tube luminometer (Berthold detection systems ...
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bioRxiv - Cancer Biology 2023Quote: ... and performing the Dual-Luciferase Reporter Assay System (E1910; Promega) after 72 h as previously described18.
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured 24 h after plating (T0) and after 4 d using the CellTiter-Glo Luminescent Cell Viability Assay protocol (Promega; cat# G7573). When indicated ...
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bioRxiv - Biophysics 2023Quote: ... using Dual-Luciferase Reporter Assay System (Promega, #E1910) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day 100 μl of each sample was transferred to a white 96-well plate and 100 μl of the the mix of Bright-GloTM Luciferase Assay (Promega) was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... the G9281 rabbit anti-HaloTag antibody (Promega) was used at a dilution in the range of 1:30 to 1:500 (1:500 is the working dilution for immunofluorescence).
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bioRxiv - Cancer Biology 2023Quote: ... a sequencing grade trypsin (Promega), diluted in 50 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcriptional activation activity of Cul9 was detected by applying a luciferase kit (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Each organ was homogenized with a lysis solution containing a 1:5 dilution of Luciferase Cell Culture Lysis 5X Reagent (Promega, Madison, WI, USA, cat. no. E1531) in Millipore water supplemented with 1 % (v/v ...
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bioRxiv - Biophysics 2023Quote: ... on the Glomax Luminometer (Promega). The relative luciferase activity was calculated by normalizing firefly luciferase activity to the Renilla luciferase activity to control for transfection efficiency.
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bioRxiv - Biophysics 2023Quote: ... A pRL-SV40 vector (Promega #E2261) expressing Renilla luciferase with SV40 promoter was used as a control to normalize luciferase activity ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from single cell suspensions of thymic leukemias using the Wizard Genomic DNA Purification Kit (Promega, Madison WI). PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio ...
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bioRxiv - Biophysics 2023Quote: Transfected cells were stained with JF-549 tagged HaloTag ligand (Promega) and Vybrant DiO staining solution (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... cells were transiently transfected using 1g of plasmid and 3ul of FuGENE HD transfection reagent (Promega) based on manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Eighty microliters of rRNA-depleted RNA were treated with RQ1 RNAse-free DNAse (Promega, USA) (10uL DNAse + 10uL buffer ...
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bioRxiv - Biophysics 2023Quote: To investigate CBD influence on the cells viability and metabolic activity the cells were tested using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) with tetrazolium compound ...
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bioRxiv - Cancer Biology 2023Quote: ... The total RNA was treated with RQ1 RNase-Free DNase (Promega, #M6101) at 37°C for 40 minutes and purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-rabbit IgG (Promega, #W4011). The membrane was washed three times with PBS for 10 minutes and subjected to signal detection using ECL™ Prime Western Blotting System (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used in this study were 1:2,500-diluted HRP-conjugated anti-mouse IgG (Promega, #W4021) and anti-rabbit IgG (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... mice received intraperitoneal injection of 50 μl 30 mg/ml d-luciferin (in PBS) (E1603, Promega). Continuous administration of isofluorane gas was provided to maintain anaesthesia of animals during imaging ...
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bioRxiv - Cancer Biology 2023Quote: ... and viability was quantified using cell titer glow assay (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... or the Caspase Glo® 3/7 Assay (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol and plates were read on an EnVision Multilabel Plate Reader (Perkin Elmer ...
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bioRxiv - Cancer Biology 2023Quote: ... ATP content was measured using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega Corporation, Madison, WI, USA) or the Caspase Glo® 3/7 Assay (Promega Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 μg of total RNA was reverse transcribed into cDNA with random hexamer and M-MLV Reverse Transcriptase H Minus (both from Promega, Madison, WI, USA) for real-time quantitative reverse transcriptase-PCR (qRT-PCR ...