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Citations for Promega :
7301 - 7350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and plates were either run in the XF Analyzer or used to measure intracellular ATP concentration with the Cell-Titer Glo Assay (Promega). ATP measurements were taken before and after activation with magnetic Dynabeads coated with anti-CD3/CD28 antibodies (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... 2X GoTaq G2 Hot Start Colorless Master Mix was purchased from Promega (9IM743). Uracil-DNA glycosylase (UDG ...
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bioRxiv - Biochemistry 2023Quote: ... DNA used for transfection was purified using the PureYield Plasmid Midiprep System (Promega). After incubation for 30 min ...
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bioRxiv - Bioengineering 2023Quote: ... and luciferase assays were performed using the Nano-Glo® Dual Reporter Assay Kit (Promega) and the GloMax®-Multi Detection luminometer System (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... and the GloMax®-Multi Detection luminometer System (Promega). The experiments were carried out in three biological replicates for the Z-Cre vs ZZ-Cre ZIKV replicase comparison experiment and once for the ZZ-Cre NIEV ...
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bioRxiv - Biochemistry 2023Quote: ... using Cell Titer-Blue Assay Reagent (Promega) at fluorescence intensity (579Ex/584Em) ...
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bioRxiv - Biochemistry 2023Quote: ... and reporter expression was quantified using a Tecan Spark and Dual-Glo® Luciferase Assay System (Promega, E2920) according to a manufacturers instruction.
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.01% ProteaseMax (Promega Cat #V2071). The beads were centrifuged at 2000 rpm for 5 minutes to separate the beads from the supernatant ...
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bioRxiv - Bioengineering 2023Quote: ... Using the luciferase assay system (Promega), the organ specific luminescence was quantified ...
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bioRxiv - Bioengineering 2023Quote: ... the Bio-Glo™ Luciferase Assay System (Promega, UK) was added to each well and incubated in the dark for 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... RBD proteins in culture media were measured in Nano-Glo buffer (Promega N1110) supplemented with Nanoluciferase substrate and 11S protein according to the manual ...
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bioRxiv - Biochemistry 2023Quote: ... stable HEK293T cells expressing wild type COMT were transiently transfected with pTagBFP-C expressing the KDEL marker using FugeneHD (Promega) following the manufacturer’s instructions with 100 ng plasmid ...
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bioRxiv - Bioengineering 2023Quote: ... Bio-Glo™ Luciferase Assay System (Promega, UK) was added to each well and incubated in the dark for 15 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... cell viability was determined via Cell-Titre-Glo assay (Promega) according to manufacturer’s protocol for viability determination ...
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bioRxiv - Bioengineering 2023Quote: ... we measured cell viability by image analysis and using Cell-Titre-Glo kit (Promega) to detect metabolically active cells ...
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bioRxiv - Bioengineering 2023Quote: ... We then performed first-strand cDNA synthesis from 400 ng total RNA in 20 uL reactions using Promega GoScript Reverse Transcription Kit (Promega, catalog # A5000).
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bioRxiv - Microbiology 2023Quote: Viral stocks were generated in tissue culture by transfecting MCMV genome-carrying BACs into NIH3T3 cells (ATCC CRL-1658) using FuGene HD (Promega). Reconstituted viral particles were passaged five times on M2-10B4 cells (ATCC CRL-1972 ...
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bioRxiv - Biochemistry 2023Quote: ... Trypsin (Promega) was added to each sample in an estimated 50:1 protein/enzyme ratio before they were incubated on a rocker at room temperature for 14 hours ...
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bioRxiv - Biochemistry 2023Quote: ... All samples were then diluted 8-fold with 100 mM NH4HCO3 and 1 mM CaCl2 and digested with sequencing-grade modified porcine trypsin (Promega, WI, USA) provided at a 1 ...
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bioRxiv - Biochemistry 2023Quote: ... mglB-Strep-Operon-mglA vector treated with TSAP (Promega) and the digested mglA-link-mglB-H6 insert was ligated at the MglA XhoI restriction site using T4 DNA Ligase ...
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bioRxiv - Biochemistry 2023Quote: ... Northern probes were generated from gel-purified PCR products corresponding to the RNAi inserts and radioactively labelled using the Prime-a-Gene labelling system (Promega).
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bioRxiv - Biochemistry 2023Quote: ... The number of viable cells was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega G3580) and absorbance at 490 nm was measured by a plate reader (CLARIOstar ...
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bioRxiv - Bioengineering 2023Quote: ... and the Hot start GoTaq polymerase (Promega Corporation, USA) were used for PCR according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... Cell viability was measured using Cell Titer Glo kit from Promega (G9681). Reduction in luciferase counts due to the small molecule was normalised to the cell viability.
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bioRxiv - Biochemistry 2023Quote: ... After that pancreatic enzyme was added at a ratio of 1:50 (Trypsin Gold, Promega, Fitchburg, WI, USA), and incubated overnight at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cell viabilities were determined using the CellTiter-Glo® assay kit (Promega G7558). Cells were cultured and seeded onto 384-well assay plate (Corning 3765 ...
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bioRxiv - Biochemistry 2023Quote: Eluted proteins were digested with Lys-C (FUJIFILM Wako) and trypsin (Promega, Madison, WI) according to the PTS protocol21 ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM DTT containing 10 ng/μL trypsin/Lys-C mixture (Promega), and incubated overnight at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... ADP-GloTM Max assay (Promega #V7001) was used ...
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bioRxiv - Biochemistry 2023Quote: ... Lactate dehydrogenase release was detected using the CytoTox-ONE Homogenous Membrane Integrity Assay Kit (Promega), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Modified trypsin (Promega, Madison, WI, USA) stock was diluted in 100 mM AMBIC (0.1 µg/µL ...
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bioRxiv - Biochemistry 2023Quote: The pull-down samples were treated on-beads by trypsin/LysC (Promega). The resulting peptides were loaded and separated on a C18 columns for online liquid chromatography performed with an RSLCnano system (Ultimate 3000 ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing-grade modified trypsin was purchased from Promega (Madison, WI). Water was obtained from a Millipore Milli-Q system (Bedford ...
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bioRxiv - Biochemistry 2023Quote: ... The dried pieces were wetted in 10 ng/µL trypsin gold (Promega) in 25 mM NH4HCO3 and incubated at 37°C overnight (16 h) ...
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bioRxiv - Biochemistry 2023Quote: Cholesterol extraction was measured using the cholesterol/ cholesterol Ester-GloTM assay (Promega, Madison, USA). 25 mg of PFA fixed mouse liver was incubated in 3 ml of 1% w/v different cyclodextrin-containing antibody buffers ...
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bioRxiv - Biochemistry 2023Quote: ... and mixed with the nanoluciferase substrate Nano-Glo (Promega, N1120, final dilution 1:200) before measuring luminescence ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.4) with nanoluciferase substrate Nano-Glo (Promega, final dilution 1:200). Luminescence signals at 460 ± 80 and 535 ± 35 nm were measured at 28 °C every 0.96 s in a BMG Labtech POLARStar Omega plate reader ...
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bioRxiv - Biochemistry 2023Quote: ... CellTiter-Glo luminescent cell viability assay (Promega) was mixed 1:1 with culture medium and 200 µl/well was used to quantify viability of monocultures ...
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bioRxiv - Biochemistry 2023Quote: ... Viability was quantified with CellTiter-Glo luminescent cell viability assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... purified PsVs were treated with DNase I (PROMEGA) to remove unencapsidated DNA associated with capsids ...
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bioRxiv - Biochemistry 2023Quote: ... 8 μg of trypsin (Promega) was added ...
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bioRxiv - Biochemistry 2023Quote: ... CellTox Green Dye (CellTox™ Green Cytotoxicity Assay, Promega), either 2.5 mM or 100 µM of P[5]a ...
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bioRxiv - Biochemistry 2023Quote: ... samples were washed twice with S-Trap buffer before overnight digestion at 37° C with Trypsin (Promega Cat# V511X) in digestion buffer (50 mM ammonium carbonate ...
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bioRxiv - Biochemistry 2023Quote: ... and trypsin (Promega) at a ratio of 1:100 (w/w ...
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bioRxiv - Biophysics 2023Quote: ... followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl of pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: Tryptic digestion was performed at 37° C with 125 μL Trypsin (Promega Cat# V511X) in digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2023Quote: ... with purified LgBiT Protein (Promega). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then digested with trypsin (11 μL of 0.5 μg/μL per sample, resuspended in trypsin resuspension buffer containing 20 mM CaCl2; Promega, #V542A) overnight at 37 °C in a shaker incubator ...
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bioRxiv - Biochemistry 2023Quote: ... before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg/μL sequencing-grade trypsin (Promega). Tryptic peptides were eluted ...